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Fig. 1. Hemorrhage results in increased high mobility group box 1 (HMGB1) expression in the lung and plasma. A: amounts of HMGB1 and -actin in the lungs were determined by Western blot analysis at the designated time points after hemorrhage. A representative experiment is shown. B: hemorrhage induced increases in pulmonary HMGB1 concentrations. Results show the HMGB1/-actin ratio (means ± SE) from Western blots performed on groups of 3 mice at each time point. C: serum HMGB1 levels as measured by ELISA. Each experimental group consisted of 5 mice. Values are shown means ± SE. *P < 0.05 vs. control. Cont, control.