AJP - Lung Cellular and Molecular Physiology, Vol 260, Issue 2 61-L67, Copyright © 1991 by American Physiological Society

ARTICLES

Effects of P. aeruginosa-derived bacterial products on tracheal ciliary function: role of O2 radicals

J. T. Jackowski, Z. Szepfalusi, D. A. Wanner, Z. Seybold, M. W. Sielczak, I. T. Lauredo, T. Adams, W. M. Abraham and A. Wanner
Pulmonary Division, University of Miami, Mount Sinai Medical Center, Miami Beach, Florida 33140.

The purpose of this investigation was to evaluate the effects of bacterial products derived from Pseudomonas aeruginosa on the function of airway cilia and to assess the role of phagocytes and oxygen radicals in the observed responses. Ciliary beat frequency (CBF) was measured in a perfusion chamber with a microscopic technique using tracheal epithelial cells obtained from normal sheep by brush biopsy (70% epithelial cells, 18% macrophages, 11% neutrophils). Baseline CBF ranged between 678 and 1,126 min-1. After 20 min of perfusion with the cell free supernatant of P. aeruginosa culture (mucoid strain), a concentration-dependent depression of CBF was observed with a 58% inhibition at a 1:1 dilution (P less than 0.05). The P. aeruginosa-derived products pyocyanin and 1-hydroxyphenazine also decreased CBF in a dose-related fashion. The cilion-inhibitory effects of the supernatant and bacterial products were markedly attenuated after centrifugation of the brush preparation (80% epithelial cells, 16.5% macrophages, 3.5% neutrophils). Glucose/glucose oxidase also caused a rapid, concentration-dependent cilioinhibition or ciliostasis. Catalase blocked or attenuated the ciliary effects of the supernatant, bacterial products and glucose/glucose oxidase. Thus bacterial products released from P. aeruginosa impaired ciliary activity by a pathway which involved neutrophils and was mediated by toxic oxygen radicals.

This article has been cited by other articles:

				Y. Q. O'Malley, K. J. Reszka, G. T. Rasmussen, M. Y. Abdalla, G. M. Denning, and B. E. Britigan The Pseudomonas secretory product pyocyanin inhibits catalase activity in human lung epithelial cells Am J Physiol Lung Cell Mol Physiol, November 1, 2003; 285(5): L1077 - L1086. [Abstract] [Full Text] [PDF]

				G. M. Denning, S. S. Iyer, K. J. Reszka, Y. O'Malley, G. T. Rasmussen, and B. E. Britigan Phenazine-1-carboxylic acid, a secondary metabolite of Pseudomonas aeruginosa, alters expression of immunomodulatory proteins by human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L584 - L592. [Abstract] [Full Text] [PDF]

				Y. Q. O'Malley, M. Y. Abdalla, M. L. McCormick, K. J. Reszka, G. M. Denning, and B. E. Britigan Subcellular localization of Pseudomonas pyocyanin cytotoxicity in human lung epithelial cells Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L420 - L430. [Abstract] [Full Text] [PDF]

				A. Van der Vliet, M. N. Nguyen, M. K. Shigenaga, J. P. Eiserich, G. P. Marelich, and C. E. Cross Myeloperoxidase and protein oxidation in cystic fibrosis Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L537 - L546. [Abstract] [Full Text] [PDF]

				R. A. Hirst, K. S. Sikand, A. Rutman, T. J. Mitchell, P. W. Andrew, and C. O'Callaghan Relative Roles of Pneumolysin and Hydrogen Peroxide from Streptococcus pneumoniae in Inhibition of Ependymal Ciliary Beat Frequency Infect. Immun., March 1, 2000; 68(3): 1557 - 1562. [Abstract] [Full Text] [PDF]

				I. T. Lauredo, J. R. Sabater, A. Ahmed, Y. Botvinnikova, and W. M. Abraham Mechanism of pyocyanin- and 1-hydroxyphenazine-induced lung neutrophilia in sheep airways J Appl Physiol, December 1, 1998; 85(6): 2298 - 2304. [Abstract] [Full Text] [PDF]

				D. Li, M. Gallup, N. Fan, D. E. Szymkowski, and C. B. Basbaum Cloning of the Amino-terminal and 5'-Flanking Region of the Human MUC5AC Mucin Gene and Transcriptional Up-regulation by Bacterial Exoproducts J. Biol. Chem., March 20, 1998; 273(12): 6812 - 6820. [Abstract] [Full Text] [PDF]