| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Lung Cellular and Molecular Physiology, Vol 267, Issue 6 660-L666, Copyright © 1994 by American Physiological Society
ARTICLES |
S. Weissbach, A. Neuendank, M. Pettersson, T. Schaberg and U. Pison
Department of Anesthesiology and Intensive Care Medicine, Universitatsklinikum Rudolf Virchow, Freie Universitat, Berlin, Germany.
The production and release of reactive oxygen species (the respiratory burst) is a common metabolic pathway linked to several macrophage-related reactions. The most abundant surfactant protein A (SP-A) binds to alveolar macrophages (AM) through a specific surface receptor with high affinity. Because such binding might initiate or modulate the respiratory burst, we wanted to know whether and how SP-A affects the oxygen radical release from AM. To answer these questions, we measured the release of reactive oxygen species from rat AM under various in vitro conditions using enhanced chemiluminescence systems. We prepared SP-A from pulmonary surfactant isolated either from silica-treated rats or adult dogs. Resident AM were harvested from pathogen-free Wistar rats by lung lavage. Adhered and nonadhered AM were assessed on protein-free or protein-coated surfaces of 96-well microtiter plates. On protein-free surfaces, the sole addition of SP-A failed to induce measurable oxygen radical release from 2 x 10(5) adhered or nonadhered AM, while zymosan opsonized with SP-A induced a marked increase over control. On protein-coated surfaces, AM respond differently depending on the coated protein: on SP-A-coated surfaces, a dose-dependent enhancement of oxygen radical release with a mean effective concentration of approximately 1.15 micrograms/ml was found. No such enhancement was seen on plates coated with similar amounts of either human fibronectin or collagen, and the enhancement with serum albumin was not dose related. Our data demonstrate that SP-A only enhances oxygen radical release from AM if SP-A is fixed to zymosan or the surface of the reaction vial in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  J. E. Crowther, V. K. Kutala, P. Kuppusamy, J. S. Ferguson, A. A. Beharka, J. L. Zweier, F. X. McCormack, and L. S. Schlesinger Pulmonary Surfactant Protein A Inhibits Macrophage Reactive Oxygen Intermediate Production in Response to Stimuli by Reducing NADPH Oxidase Activity J. Immunol., June 1, 2004; 172(11): 6866 - 6874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Yu and F. Possmayer Lipid compositional analysis of pulmonary surfactant monolayers and monolayer-associated reservoirs J. Lipid Res., March 1, 2003; 44(3): 621 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Korfhagen Surfactant Protein A (SP-A)-Mediated Bacterial Clearance . SP-A and Cystic Fibrosis Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 668 - 672. [Full Text] [PDF]
|
|
|
|
|
|
L. Salez, V. Balloy, N. van Rooijen, M. Lebastard, L. Touqui, F. X. McCormack, and M. Chignard Surfactant Protein A Suppresses Lipopolysaccharide-Induced IL-10 Production by Murine Macrophages J. Immunol., May 15, 2001; 166(10): 6376 - 6382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. F. Weikert, J. P. Lopez, R. Abdolrasulnia, Z. C. Chroneos, and V. L. Shepherd Surfactant protein A enhances mycobacterial killing by rat macrophages through a nitric oxide-dependent pathway Am J Physiol Lung Cell Mol Physiol, August 1, 2000; 279(2): L216 - L223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Pinot, H. Walti, H. P. Haagsman, B. S. Polla, and M. Bachelet Curosurf modulates cAMP accumulation in human monocytes through a membrane-controlled mechanism Am J Physiol Lung Cell Mol Physiol, January 1, 2000; 278(1): L99 - L104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Bruno, J. A. Whitsett, G. F. Ross, and T. R. Korfhagen Transcriptional Regulation of the Murine Surfactant Protein-A Gene by B-Myb J. Biol. Chem., September 24, 1999; 274(39): 27523 - 27528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Schagat, M. J. Tino, and J. R. Wright Regulation of Protein Phosphorylation and Pathogen Phagocytosis by Surfactant Protein A Infect. Immun., September 1, 1999; 67(9): 4693 - 4699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Elhalwagi, M. Zhang, M. Ikegami, H. S. Iwamoto, R. E. Morris, M. L. Miller, K. Dienger, and F. X. McCormack Normal Surfactant Pool Sizes and Inhibition-Resistant Surfactant from Mice That Overexpress Surfactant Protein A Am. J. Respir. Cell Mol. Biol., September 1, 1999; 21(3): 380 - 387. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Hickman-Davis, J. Gibbs-Erwin, J. R. Lindsey, and S. Matalon Surfactant protein A mediates mycoplasmacidal activity of alveolar macrophages by production of peroxynitrite PNAS, April 27, 1999; 96(9): 4953 - 4958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. LeVine, K. E. Kurak, J. R. Wright, W. T. Watford, M. D. Bruno, G. F. Ross, J. A. Whitsett, and T. R. Korfhagen Surfactant Protein-A Binds Group B Streptococcus Enhancing Phagocytosis and Clearance from Lungs of Surfactant Protein-A-Deficient Mice Am. J. Respir. Cell Mol. Biol., February 1, 1999; 20(2): 279 - 286. [Abstract] [Full Text]
|
|
|
|
|
|
S. Zhu, D. L. Kachel, W. J. Martin II, and S. Matalon Nitrated SP-A does not enhance adherence of Pneumocystis carinii to alveolar macrophages Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1031 - L1039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Borron, F. X. McCormack, B. M. Elhalwagi, Z. C. Chroneos, J. F. Lewis, S. Zhu, J. R. Wright, V. L. Shepherd, F. Possmayer, K. Inchley, et al. Surfactant protein A inhibits T cell proliferation via its collagen-like tail and a 210-kDa receptor Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L679 - L686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Kresch, C. Christian, and H. Lu Isolation and Partial Characterization of a Receptor to Surfactant Protein A Expressed by Rat Type II Pneumocytes Am. J. Respir. Cell Mol. Biol., August 1, 1998; 19(2): 216 - 225. [Abstract] [Full Text]
|
|
|
|
|
|
R. J. Mason, K. Greene, and D. R. Voelker Surfactant protein A and surfactant protein D in health and disease Am J Physiol Lung Cell Mol Physiol, July 1, 1998; 275(1): L1 - L13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Hickman-Davis, J. R. Lindsey, S. Zhu, and S. Matalon Surfactant protein A mediates mycoplasmacidal activity of alveolar macrophages Am J Physiol Lung Cell Mol Physiol, February 1, 1998; 274(2): L270 - L277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Israel-Assayag and Y. Cormier Surfactant modifies the lymphoproliferative activity of macrophages in hypersensitivity pneumonitis Am J Physiol Lung Cell Mol Physiol, December 1, 1997; 273(6): L1258 - L1264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. T. Yuan, S. Gowan, F. J. Kelly, and C. D. Bingle Cloning of guinea pig surfactant protein A defines a distinct cellular distribution pattern within the lung Am J Physiol Lung Cell Mol Physiol, October 1, 1997; 273(4): L900 - L906. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
