AJP - Lung Cellular and Molecular Physiology, Vol 269, Issue 6 754-L765, Copyright © 1995 by American Physiological Society

ARTICLES

Composition of extracellular matrix of type II pulmonary epithelial cells in primary culture

S. E. Dunsmore, C. Martinez-Williams, R. A. Goodman and D. E. Rannels
Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033, USA.

Type II pulmonary epithelial cells in primary culture synthesize and deposit an extracellular matrix which has reciprocal biological effects on cellular differentiation. The present work establishes conditions for metabolic labeling of matrix constituents and for separation of cells from the associated matrix; it also defines matrix composition, which does not appear to change qualitatively between days 2 and 6 of primary culture. Type II cells synthesize and deposit a spectrum of radiolabeled components on the culture surface. These include fibronectin, laminin, type IV collagen, and plasminogen activator inhibitor-1, along with additional unidentified proteins. Few radiolabeled proteins in medium conditioned by type II cells bind nonspecifically to the culture surface in the absence of cells. Fibroblasts and macrophages, which may contaminate the primary cultures, do not appear to contribute substantially to the type II cell matrix. These results demonstrate that type II cells synthesize and deposit a complex multicomponent extracellular matrix. The work provides a basis for further investigations of bidirectional interactions between type II cells and the extracellular matrix.

This article has been cited by other articles:

				J. L. Fisher and S. S. Margulies Na+-K+-ATPase activity in alveolar epithelial cells increases with cyclic stretch Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L737 - L746. [Abstract] [Full Text] [PDF]

				J. Oswari, M. A. Matthay, and S. S. Margulies Keratinocyte growth factor reduces alveolar epithelial susceptibility to in vitro mechanical deformation Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1068 - L1077. [Abstract] [Full Text] [PDF]

				A. I. Alford and D. E. Rannels Extracellular matrix fibronectin alters connexin43 expression by alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L680 - L688. [Abstract] [Full Text] [PDF]

				Y. Guo, C. Martinez-Williams, C. E. Yellowley, H. J. Donahue, and D. E. Rannels Connexin expression by alveolar epithelial cells is regulated by extracellular matrix Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L191 - L202. [Abstract] [Full Text] [PDF]

				S. Rosseau, J. Selhorst, K. Wiechmann, K. Leissner, U. Maus, K. Mayer, F. Grimminger, W. Seeger, and J. Lohmeyer Monocyte Migration Through the Alveolar Epithelial Barrier: Adhesion Molecule Mechanisms and Impact of Chemokines J. Immunol., January 1, 2000; 164(1): 427 - 435. [Abstract] [Full Text] [PDF]

				T. J. Mariani, M. C. Arikan, and R. A. Pierce Fibroblast Tropoelastin and alpha -Smooth-Muscle Actin Expression Are Repressed by Particulate-Activated Macrophage-Derived Tumor Necrosis Factor-alpha in Experimental Silicosis Am. J. Respir. Cell Mol. Biol., August 1, 1999; 21(2): 185 - 192. [Abstract] [Full Text]

				Y.-C. Lee and D. E. Rannels Regulation of extracellular matrix synthesis by TNF-alpha and TGF-beta 1 in type II cells exposed to coal dust Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L637 - L644. [Abstract] [Full Text] [PDF]

				T. J. Mariani, S. Sandefur, J. D. Roby, and R. A. Pierce Collagenase-3 Induction in Rat Lung Fibroblasts Requires the Combined Effects of Tumor Necrosis Factor-alpha and 12-Lipoxygenase Metabolites: A Model of Macrophage-induced, Fibroblast-driven Extracellular Matrix Remodeling during Inflammatory Lung Injury Mol. Biol. Cell, June 1, 1998; 9(6): 1411 - 1424. [Abstract] [Full Text]

				W. M. Maniscalco, R. H. Watkins, P. R. Chess, R. A. Sinkin, S. Horowitz, and L. Toia Cell-specific expression of fibronectin and EIIIA and EIIIB splice variants after oxygen injury Am J Physiol Lung Cell Mol Physiol, April 1, 1998; 274(4): L599 - L609. [Abstract] [Full Text] [PDF]

				T. J. Mariani, S. E. Dunsmore, Q. Li, X. Ye, and R. A. Pierce Regulation of lung fibroblast tropoelastin expression by alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 1998; 274(1): L47 - L57. [Abstract] [Full Text] [PDF]

				P. L. Sannes, J. Khosla, and B. P. Peters Biosynthesis of sulfated extracellular matrices by alveolar type II cells increases with time in culture Am J Physiol Lung Cell Mol Physiol, October 1, 1997; 273(4): L840 - L847. [Abstract] [Full Text] [PDF]

				N. M. Nguyen, Y. Bai, K. Mochitate, and R. M. Senior Laminin alpha -chain expression and basement membrane formation by MLE-15 respiratory epithelial cells Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L1004 - L1011. [Abstract] [Full Text] [PDF]