AJP - Lung Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 269: L791-L799, 1995;
1040-0605/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stripp, B. R.
Right arrow Articles by Singh, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stripp, B. R.
Right arrow Articles by Singh, G.

AJP - Lung Cellular and Molecular Physiology, Vol 269, Issue 6 791-L799, Copyright © 1995 by American Physiological Society

ARTICLES

Plasticity of airway cell proliferation and gene expression after acute naphthalene injury

B. R. Stripp, K. Maxson, R. Mera and G. Singh
Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.

The goal of this study was to determine the temporal and spatial sequence of events that accompany lung injury and repair after parenteral administration of the Clara cell-specific cytotoxicant, naphthalene. Changes in airway epithelial cells were evaluated by measuring alterations in the expression of markers for differentiated Clara cells (CYPIIF and Clara cell 10-kDa secretory protein, CC10), distal airway/alveolar type II cells (surfactant protein B; SP-B) and for cycling/proliferating cells (cyclin dependent kinase 1;CDK1). Naphthalene-induced Clara cell cytotoxicity resulted in the exfoliation of epithelial cells containing CC10 protein. This was accompanied by a dramatic reduction in the abundance of mRNA for CC10 and CYPIIF. Large numbers of CDK1 mRNA-positive cells were identified in and around bronchioles and terminal bronchioles 48 h after treatment. This cellular proliferation resulted in the population of airways by immature epithelial cells lacking normal levels of CC10 mRNA but overexpressing SP-B mRNA. Seventy-two hours after naphthalene treatment a reduction in CDK1 mRNA-positive cells was noted within bronchioles and terminal bronchioles at all locations, with the exception of airway bifurcations. At airway bifurcations CDK1 mRNA appeared to be more abundant at the 72-h time point than at 48 h. Comparison of these sections with serial sections probed for CC10 mRNA demonstrated a correlation between the expression of CDK1 and CC10 mRNA at bifurcations. Temporal increases in the abundance of CC10 mRNA observed at later time points were largely accounted for by the processive maturation of newly repopulated cells neighboring bifurcations in bronchioles. These studies identify spatially distinct populations of cells that act in concert to repopulate naphthalene-injured airways and support the notion that branch point cells play an important role in the maturation of newly regenerated airway epithelial cells after acute injury.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. E. Adair, V. Stober, M. Sobhany, L. Zhuo, J. D. Roberts, M. Negishi, K. Kimata, and S. Garantziotis
Inter-{alpha}-trypsin Inhibitor Promotes Bronchial Epithelial Repair after Injury through Vitronectin Binding
J. Biol. Chem., June 19, 2009; 284(25): 16922 - 16930.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. J. Atkinson, H. M. Toennies, K. Holmbeck, and R. M. Senior
Membrane type 1 matrix metalloproteinase is necessary for distal airway epithelial repair and keratinocyte growth factor receptor expression after acute injury
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L600 - L610.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. P. Wong, A. E. Dutly, A. Sacher, H. Lee, D. M. Hwang, M. Liu, S. Keshavjee, J. Hu, and T. K. Waddell
Targeted cell replacement with bone marrow cells for airway epithelial regeneration
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L740 - L752.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. D. Reynolds, A. Giangreco, K. U. Hong, K. E. McGrath, L. A. Ortiz, and B. R. Stripp
Airway injury in lung disease pathophysiology: selective depletion of airway stem and progenitor cell pools potentiates lung inflammation and alveolar dysfunction
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1256 - L1265.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. U. Hong, S. D. Reynolds, S. Watkins, E. Fuchs, and B. R. Stripp
In vivo differentiation potential of tracheal basal cells: evidence for multipotent and unipotent subpopulations
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L643 - L649.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Yoshikawa, J. A. King, S. D. Reynolds, B. R. Stripp, and J. C. Parker
Time and pressure dependence of transvascular Clara cell protein, albumin, and IgG transport during ventilator-induced lung injury in mice
Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L604 - L612.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. D. Reynolds, P. R. Reynolds, G. S. Pryhuber, J. D. Finder, and B. R. Stripp
Secretoglobins SCGB3A1 and SCGB3A2 Define Secretory Cell Subsets in Mouse and Human Airways
Am. J. Respir. Crit. Care Med., December 1, 2002; 166(11): 1498 - 1509.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Giangreco, S. D. Reynolds, and B. R. Stripp
Terminal Bronchioles Harbor a Unique Airway Stem Cell Population That Localizes to the Bronchoalveolar Duct Junction
Am. J. Pathol., July 1, 2002; 161(1): 173 - 182.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. S. Van Winkle, A. D. Gunderson, J. A. Shimizu, G. L. Baker, and C. D. Brown
Gender differences in naphthalene metabolism and naphthalene-induced acute lung injury
Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L1122 - L1134.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Vuong, T. Patterson, P. Adiseshaiah, P. Shapiro, D. V. Kalvakolanu, and S. P. M. Reddy
JNK1 and AP-1 regulate PMA-inducible squamous differentiation marker expression in Clara-like H441 cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L215 - L225.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. W. Lawson, L. S. Van Winkle, E. Toskala, R. M. Senior, W. C. Parks, and C. G. Plopper
Mouse Strain Modulates the Role of the Ciliated Cell in Acute Tracheobronchial Airway Injury-Distal Airways
Am. J. Pathol., January 1, 2002; 160(1): 315 - 327.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. M. Watson, S. D. Reynolds, G. W. Mango, I.-M. Boe, J. Lund, and B. R. Stripp
Altered lung gene expression in CCSP-null mice suggests immunoregulatory roles for Clara cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1523 - L1530.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. U. Hong, S. D. Reynolds, A. Giangreco, C. M. Hurley, and B. R. Stripp
Clara Cell Secretory Protein-Expressing Cells of the Airway Neuroepithelial Body Microenvironment Include a Label-Retaining Subset and Are Critical for Epithelial Renewal after Progenitor Cell Depletion
Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 671 - 681.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
L. S. Van Winkle, M. J. Evans, C. D. Brown, N. H. Willits, K. E. Pinkerton, and C. G. Plopper
Prior Exposure to Aged and Diluted Sidestream Cigarette Smoke Impairs Bronchiolar Injury and Repair
Toxicol. Sci., March 1, 2001; 60(1): 152 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. W. Wright, R. H. Notter, Z. Wang, A. G. Harmsen, and F. Gigliotti
Pulmonary Inflammation Disrupts Surfactant Function during Pneumocystis carinii Pneumonia
Infect. Immun., February 1, 2001; 69(2): 758 - 764.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. D. Reynolds, K. U. Hong, A. Giangreco, G. W. Mango, C. Guron, Y. Morimoto, and B. R. Stripp
Conditional Clara cell ablation reveals a self-renewing progenitor function of pulmonary neuroendocrine cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1256 - L1263.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. ARSALANE, F. BROECKAERT, B. KNOOPS, M. WIEDIG, G. TOUBEAU, and A. BERNARD
Clara Cell Specific Protein (CC16) Expression after Acute Lung Inflammation Induced by Intratracheal Lipopolysaccharide Administration
Am. J. Respir. Crit. Care Med., May 1, 2000; 161(5): 1624 - 1630.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
S. D. Reynolds, A. Giangreco, J. H. T. Power, and B. R. Stripp
Neuroepithelial Bodies of Pulmonary Airways Serve as a Reservoir of Progenitor Cells Capable of Epithelial Regeneration
Am. J. Pathol., January 1, 2000; 156(1): 269 - 278.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. W. Mango, C. J. Johnston, S. D. Reynolds, J. N. Finkelstein, C. G. Plopper, and B. R. Stripp
Clara cell secretory protein deficiency increases oxidant stress response in conducting airways
Am J Physiol Lung Cell Mol Physiol, August 1, 1998; 275(2): L348 - L356.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. A. O'Reilly, R. J. Staversky, B. R. Stripp, and J. N. Finkelstein
Exposure to Hyperoxia Induces p53 Expression in Mouse Lung Epithelium
Am. J. Respir. Cell Mol. Biol., January 1, 1998; 18(1): 43 - 50.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online