| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Lung Cellular and Molecular Physiology, Vol 272, Issue 4 644-L650, Copyright © 1997 by American Physiological Society
ARTICLES |
C. W. Emala, J. Kuhl, C. L. Hungerford and C. A. Hirshman
Department of Anesthesiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA.
Inflammation, increased cytokine production, and decreased responsiveness of airway smooth muscle (ASM) to beta-adrenergic agonists are characteristics of asthma. We questioned whether the cytokine tumor necrosis factor-alpha (TNF-alpha) directly impaired beta-adrenergic signal transduction in cultured canine ASM cells. Confluent ASM cells exposed to TNF-alpha (0.1-10 ng/ml) for 72 h showed lower maximal levels of adenylyl cyclase activity in response to isoproterenol (10 ng/ml; 14 +/- 4.3 vs. 7.5 +/- 1.3 pmol adenosine 3',5'-cyclic monophosphate x well(-1) x 20 min(-1), control vs. treated, respectively), despite no changes in beta-adrenergic receptor numbers (maximum number of binding sites = 4.8 +/- 0.72 vs. 4.5 +/- 0.81 fmol/mg protein, control vs. treated, respectively). Adenylyl cyclase activities in response to prostaglandin E1, NaF, or forskolin were not different in treated and untreated cells. These results demonstrate that a cytokine known to be increased during exacerbation of asthmatic symptoms directly impairs beta-adrenergic function in ASM cells and suggests a mechanism by which inflammation impairs beta-adrenergic receptor signal transduction in asthma.
This article has been cited by other articles:
|
|
 |
|
  E. Baloglu, A. Ke, I. H. Abu-Taha, P. Bartsch, and H. Mairbaurl In vitro hypoxia impairs {beta}2-adrenergic receptor signaling in primary rat alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L500 - L509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Deshpande, T. A. White, S. Dogan, T. F. Walseth, R. A. Panettieri, and M. S. Kannan CD38/cyclic ADP-ribose signaling: role in the regulation of calcium homeostasis in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L773 - L788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen, O. Tliba, C. R. Van Besien, R. A. Panettieri Jr., and Y. Amrani Selected Contribution: TNF-{alpha} modulates murine tracheal rings responsiveness to G-protein-coupled receptor agonists and KCl J Appl Physiol, August 1, 2003; 95(2): 864 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Hunter, H. J. Cobban, P. Vandenabeele, D. J. MacEwan, and G. F. Nixon Tumor Necrosis Factor-alpha -Induced Activation of RhoA in Airway Smooth Muscle Cells: Role in the Ca2+ Sensitization of Myosin Light Chain20 Phosphorylation Mol. Pharmacol., March 1, 2003; 63(3): 714 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sakao, K. Tatsumi, H. Igari, R. Watanabe, Y. Shino, H. Shirasawa, and T. Kuriyama Association of Tumor Necrosis Factor-{alpha} Gene Promoter Polymorphism With Low Attenuation Areas on High-Resolution CT in Patients With COPD* Chest, August 1, 2002; 122(2): 416 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Pascual, C. K. Billington, I. P. Hall, R. A. Panettieri Jr., J. E. Fish, S. P. Peters, and R. B. Penn Mechanisms of cytokine effects on G protein-coupled receptor-mediated signaling in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1425 - L1435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Hirshman, D. Zhu, R. A. Panettieri, and C. W. Emala Actin depolymerization via the beta -adrenoceptor in airway smooth muscle cells: a novel PKA-independent pathway Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1468 - C1476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Lesh, C. W. Emala, H. T. Lee, D. Zhu, R. A. Panettieri, and C. A. Hirshman Inhibition of geranylgeranylation blocks agonist-induced actin reorganization in human airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L824 - L831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. SILVERMAN, G. T. DE SANCTIS, J. BOYCE, J. A. MACLEAN, A. JIAO, F. H. Y. GREEN, H. GRASEMANN, D. FAUNCE, G. FITZMAURICE, G.-P. SHI, et al. The Transcription Factor Early Growth-response Factor 1 Modulates Tumor Necrosis Factor-{alpha}, Immunoglobulin E, and Airway Responsiveness in Mice Am. J. Respir. Crit. Care Med., March 1, 2001; 163(3): 778 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. SAKAO, K. TATSUMI, H. IGARI, Y. SHINO, H. SHIRASAWA, and T. KURIYAMA Association of Tumor Necrosis Factor {alpha} Gene Promoter Polymorphism with the Presence of Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., February 1, 2001; 163(2): 420 - 422. [Abstract] [Full Text]
|
|
|
|
|
|
K. Hotta, C. W. Emala, and C. A. Hirshman TNF-alpha upregulates Gialpha and Gqalpha protein expression and function in human airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, March 1, 1999; 276(3): L405 - L411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. KUME and K. TAKAGI Inhibition of beta -Adrenergic Desensitization by KCa Channels in Human Trachealis Am. J. Respir. Crit. Care Med., February 1, 1999; 159(2): 452 - 460. [Abstract] [Full Text]
|
|
|
|
|
|
L. Pang, E. Holland, and A. J. Knox Impaired cAMP production in human airway smooth muscle cells by bradykinin: role of cyclooxygenase products Am J Physiol Lung Cell Mol Physiol, August 1, 1998; 275(2): L322 - L329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Amrani and R. A Panettieri Jr Cytokines induce airway smooth muscle cell hyperresponsiveness to contractile agonists Thorax, August 1, 1998; 53(8): 713 - 716. [Full Text]
|
|
|
|
 |
|
 D. E. Berkowitz, D. Brown, K. M. Lee, C. Emala, D. Palmer, Y. An, and M. Breslow Endotoxin-induced alteration in the expression of leptin and beta 3-adrenergic receptor in adipose tissue Am J Physiol Endocrinol Metab, June 1, 1998; 274(6): E992 - E997. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
