| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Lung Cellular and Molecular Physiology, Vol 272, Issue 4 745-L751, Copyright © 1997 by American Physiological Society
ARTICLES |
C. W. Emala, J. Clancy and C. A. Hirshman
Department of Anesthesiology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA.
Corticosteroids upregulate the beta-adrenergic pathway, but little is known about corticosteroid regulation of muscarinic pathways. Basenji-greyhound (BG) dogs treated for 3 days with methylprednisolone (MPS) but not deoxycorticosterone (DOC) had decreased numbers of muscarinic receptors in airway smooth muscle homogenates as determined by radioligand binding with 1-[3H]quinuclidinyl benzilate (vehicle control, 578 +/- 53 fmol/mg protein; MPS, 290 +/- 22 fmol/mg protein; DOC, 565 +/- 141 fmol/mg protein). Competition radioligand binding with the M2-selective antagonist tripitramine showed a decrease in both the M2 and M3 muscarinic receptors with no changes in receptor affinities (M2: vehicle control, 478 +/- 41 fmol/mg protein; MPS, 265 +/- 20 fmol/mg protein, M3: vehicle control, 89 +/- 13 fmol/mg protein; MPS, 25 +/- 16 fmol/mg protein). In vitro treatment of airway smooth muscle from control BG dogs with MPS had no effect on muscarinic receptor number, despite increased expression of beta-adrenergic receptors. Thus glucocorticoids indirectly decrease the expression of M2 and M3 muscarinic receptors in airway smooth muscle, which, in part, may account for their beneficial effects in the treatment of asthma.
This article has been cited by other articles:
|
|
 |
|
  H. Akiho, W. I. Khan, A. Al-Kaabi, P. Blennerhassett, Y. Deng, and S. M Collins Cytokine modulation of muscarinic receptors in the murine intestine Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G250 - G255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Deshpande, T. A. White, S. Dogan, T. F. Walseth, R. A. Panettieri, and M. S. Kannan CD38/cyclic ADP-ribose signaling: role in the regulation of calcium homeostasis in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L773 - L788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Green, W. Ho, K. A. Sharkey, and D. M. McKay Dextran sodium sulfate-induced colitis reveals nicotinic modulation of ion transport via iNOS-derived NO Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G706 - G714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Billington and R. B. Penn m3 Muscarinic Acetylcholine Receptor Regulation in the Airway Am. J. Respir. Cell Mol. Biol., March 1, 2002; 26(3): 269 - 272. [Full Text] [PDF]
|
|
|
|
|
|
D. B. Jacoby, B. L. Yost, B. Kumaravel, Y. Chan-Li, H.-Q. Xiao, K. Kawashima, and A. D. Fryer Glucocorticoid Treatment Increases Inhibitory M2 Muscarinic Receptor Expression and Function in the Airways Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 485 - 491. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. J. Morris and C. C. Malbon Physiological Regulation of G Protein-Linked Signaling Physiol Rev, October 1, 1999; 79(4): 1373 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. FRYER and D. B. JACOBY Muscarinic Receptors and Control of Airway Smooth Muscle Am. J. Respir. Crit. Care Med., November 1, 1998; 158(2007): S154 - S160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. HIRST and T. H. LEE Airway Smooth Muscle as a Target of Glucocorticoid Action in the Treatment of Asthma Am. J. Respir. Crit. Care Med., November 1, 1998; 158(2007): S201 - S206. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
