AJP - Lung Cellular and Molecular Physiology, Vol 272, Issue 5 951-L958, Copyright © 1997 by American Physiological Society

ARTICLES

Vectorial transcytosis of dimeric IgA by the Calu-3 human lung epithelial cell line: upregulation by IFN-gamma

S. Loman, J. Radl, H. M. Jansen, T. A. Out and R. Lutter
Department of Pulmonology, Academic Medical Center, University of Amsterdam, The Netherlands.

We have developed an in vitro airway epithelial cell model for dimeric immunoglobulin (Ig) A (dIgA) transcytosis that allows the assessment of polymeric Ig receptor (pIgR) gene expression and actual dIgA transport. Tight monolayers of human lung-derived Calu-3 adenocarcinoma cells grown on permeable membranes expressed pIgR mRNA and released more secretory component (SC; P < 0.01) and secretory IgA (sIgA; P < 0.02) into the apical medium than into the basolateral medium. Transcytosis of dIgA was not due to paracellular leakage and was inhibited to approximately 20 and 30% of control values by anti-pIgR antibodies and the competitive ligand pentameric IgM, respectively. Interferon-gamma (IFN-gamma; 200 U/ml) induced pIgR mRNA expression and increased apical release of free SC and sIgA in a dose-dependent fashion (P < 0.0001). Basolateral addition of increasing amounts of dIgA dose dependently increased apical sIgA release (P < 0.0001). These data indicate that Calu-3 monolayers are capable of translocating dIgA through the pIgR. In addition, we show the integrated stimulatory effect of IFN-gamma on pIgR mRNA and protein expression and dIgA transcytosis.

This article has been cited by other articles:

				P. L. TUMA and A. L. HUBBARD Transcytosis: Crossing Cellular Barriers Physiol Rev, July 1, 2003; 83(3): 871 - 932. [Abstract] [Full Text] [PDF]

				M. van Wissen, M. Snoek, B. Smids, H. M. Jansen, and R. Lutter IFN-{gamma} Amplifies IL-6 and IL-8 Responses by Airway Epithelial-Like Cells Via Indoleamine 2,3-Dioxygenase J. Immunol., December 15, 2002; 169(12): 7039 - 7044. [Abstract] [Full Text] [PDF]

				T. FERKOL, E. ECKMAN, S. SWAIDANI, C. SILSKI, and P. DAVIS Transport of Bifunctional Proteins Across Respiratory Epithelial Cells via the Polymeric Immunoglobulin Receptor Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 944 - 951. [Abstract] [Full Text] [PDF]

				E. A. Eckman, W. D. Mallender, T. Szegletes, C. L. Silski, J. R. Schreiber, P. B. Davis, and T. W. Ferkol In Vitro Transport of Active alpha 1-Antitrypsin to the Apical Surface of Epithelia by Targeting the Polymeric Immunoglobulin Receptor Am. J. Respir. Cell Mol. Biol., August 1, 1999; 21(2): 246 - 252. [Abstract] [Full Text]

				L. W. Ackermann, L. A. Wollenweber, and G. M. Denning IL-4 and IFN-{gamma} Increase Steady State Levels of Polymeric Ig Receptor mRNA in Human Airway and Intestinal Epithelial Cells J. Immunol., May 1, 1999; 162(9): 5112 - 5118. [Abstract] [Full Text] [PDF]

				M. E. Lamm IV. How epithelial transport of IgA antibodies relates to host defense Am J Physiol Gastrointest Liver Physiol, April 1, 1998; 274(4): G614 - G617. [Abstract] [Full Text] [PDF]