AJP - Lung Cellular and Molecular Physiology, Vol 273, Issue 1 134-L141, Copyright © 1997 by American Physiological Society

ARTICLES

Integrin mediation of alveolar epithelial cell migration on fibronectin and type I collagen

H. J. Kim, C. A. Henke, S. K. Savik and D. H. Ingbar
Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA.

Acute lung injury leads to type I alveolar epithelial cell (AEC) death, denudation of the alveolar basement membrane, and formation of an alveolar provisional matrix from fibronectin, fibrinogen, and type I collagen. The provisional matrix provides a scaffold for alveolar repair. To restore normal lung architecture, surviving type II AECs must reepithelialize denuded alveoli. We examined whether AECs migrate on provisional matrix proteins and whether integrins mediate this migration using a Boyden chemotaxis chamber. Cultured AECs migrated on fibronectin-coated filters by haptotaxis (defined as movement on a solid-phase substrate) more than one type I collagen-coated filters, and they did not migrate on fibrinogen-coated filters. Soluble fibronectin augmented migration on type I collagen-coated filters, but not on fibronectin-coated filters. Anti-alpha v beta 3-integrin monoclonal antibody (MAb) inhibited migration on substrate-bound fibronectin by 62-77%, whereas anti-beta 1-integrin MAb inhibited migration by 48%. Anti-alpha 2-integrin MAb almost completely inhibited migration on substrate-bound type I collagen, but not on fibronectin. The novel findings in this study are as follows: 1) AECs migrate by haptotaxis more effectively on substrate-bound fibronectin than on type I collagen; 2) alpha v beta 3- and beta 1-integrins partially mediate AEC haptotaxis on fibronectin; and 3) the alpha 2 beta 1-integrin mediates AEC migration on type I collagen. These results support the importance of type II cell migration on provisional matrix proteins during repair of lung injury.

This article has been cited by other articles:

				R. K. Rowe and A. Pekosz Bidirectional Virus Secretion and Nonciliated Cell Tropism following Andes Virus Infection of Primary Airway Epithelial Cell Cultures J. Virol., February 1, 2006; 80(3): 1087 - 1097. [Abstract] [Full Text] [PDF]

				S. Buckley, B. Driscoll, W. Shi, K. Anderson, and D. Warburton Migration and gelatinases in cultured fetal, adult, and hyperoxic alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L427 - L434. [Abstract] [Full Text] [PDF]

				T. M. Odrljin, C. G. Haidaris, N. B. Lerner, and P. J. Simpson-Haidaris Integrin {alpha}v{beta}3-Mediated Endocytosis of Immobilized Fibrinogen by A549 Lung Alveolar Epithelial Cells Am. J. Respir. Cell Mol. Biol., January 1, 2001; 24(1): 12 - 21. [Abstract] [Full Text]

				T. Geiser, P.-H. Jarreau, K. Atabai, and M. A. Matthay Interleukin-1beta augments in vitro alveolar epithelial repair Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1184 - L1190. [Abstract] [Full Text] [PDF]

				R. L. Lubman, X.-L. Zhang, J. Zheng, L. Ocampo, M. Z. Lopez, S. Veeraraghavan, S. M. Zabski, S. I. Danto, and Z. Borok Integrin alpha 3-subunit expression modulates alveolar epithelial cell monolayer formation Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L183 - L193. [Abstract] [Full Text] [PDF]

				Y. Berthiaume, O. Lesur, and A. Dagenais Treatment of adult respiratory distress syndrome: plea for rescue therapy of the alveolar epithelium Thorax, February 1, 1999; 54(2): 150 - 160. [Full Text]

				N. Maeda and M. Noda Involvement of Receptor-like Protein Tyrosine Phosphatase zeta /RPTPbeta and Its Ligand Pleiotrophin/Heparin-binding Growth-associated Molecule (HB-GAM) in Neuronal Migration J. Cell Biol., July 13, 1998; 142(1): 203 - 216. [Abstract] [Full Text] [PDF]