AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 273: L478-L484, 1997;
1040-0605/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nuttle, L. C.
Right arrow Articles by Farley, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nuttle, L. C.
Right arrow Articles by Farley, J. M.

AJP - Lung Cellular and Molecular Physiology, Vol 273, Issue 2 478-L484, Copyright © 1997 by American Physiological Society

ARTICLES

Muscarinic receptors inhibit ATP-sensitive K+ channels in swine tracheal smooth muscle

L. C. Nuttle and J. M. Farley
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson 39216, USA.

The patch-clamp technique was used to characterize a cromakalim-induced current and its regulation by muscarinic receptors in tracheal smooth muscle cells. Cromakalim (10 microM) activated a steady-state increase of 33-292 pA (91.2 +/- 8.8 pA, n = 43) in whole cell current, consistent with the activation of ATP-sensitive K+ (KATP) channels. Acetylcholine (10 nM-1 microM), added cumulatively, inhibited the cromakalim-induced current by 23.6 +/- 14.9 to 73.9 +/- 4.6%. This inhibition was blocked by pretreatment with atropine (1 microM). The cromakalim-induced current was also inhibited 83.0 +/- 8.3% by phorbol 12-myristate 13-acetate (100 nM, n = 6). The inhibition of the cromakalim-induced current by acetylcholine (1 microM) and phorbol 12-myristate 13-acetate (100 nM) was reduced to 27.3 +/- 3.1% (n = 10) and 32.8 +/- 7.8% (n = 3), respectively, in the presence of staurosporine (100 microM). We conclude that muscarinic receptor stimulation inhibits KATP channel activity through the activation of protein kinase C. These findings suggest that KATP channels may play a role in the regulation of membrane potential and contractility in airway smooth muscle.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. Liu, A. M. Freyer, and I. P. Hall
Bradykinin activates calcium-dependent potassium channels in cultured human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L898 - L907.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Y. Jun, I. D. Kong, S. D. Koh, X. Y. Wang, B. A. Perrino, S. M. Ward, and K. M. Sanders
Regulation of ATP-sensitive K+ channels by protein kinase C in murine colonic myocytes
Am J Physiol Cell Physiol, September 1, 2001; 281(3): C857 - C864.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C.-C. Shieh, J. Feng, S. A. Buckner, J. D. Brioni, M. J. Coghlan, J. P. Sullivan, and M. Gopalakrishnan
Functional Implication of Spare ATP-Sensitive K+ Channels in Bladder Smooth Muscle Cells
J. Pharmacol. Exp. Ther., March 1, 2001; 296(3): 669 - 675.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online