AJP - Lung Cellular and Molecular Physiology, Vol 273, Issue 6 1203-L1207, Copyright © 1997 by American Physiological Society
Mucoglycoprotein hypersecretion in allergic rhinitis and cystic fibrosis
A. Yuta, M. Ali, M. Sabol, E. Gaumond and J. N. Baraniuk
Division of Rheumatology, Immunology, and Allergy, Georgetown University Medical Center, Washington, District of Columbia 20007-2197, USA.
There is little information about specific changes in submucosal gland
exocytosis in diseases such as allergic rhinitis (AR), nonallergic rhinitis
(NAR), and cystic fibrosis (CF). Nasal lavage fluids were collected from
normal, AR, NAR, and CF subjects. Concentrations of lysozyme, Alcian
blue-staining mucoglycoconjugate material (AB + m), and human
high-molecular-weight mucoglycoconjugates recognized by the 7F10 murine
monoclonal antibody [7F10-immunoreactive mucoglycoconjugates (7F10-irm)]
were measured. AB + m and 7F10-irm were characterized by Sepharose-2B
column chromatography and glycosidase digestion. 7F10-irm was increased in
CF (2.4-fold; P = 0.001) and AR (12.7-fold; P = 0.00007) subjects. AB + m
was increased in CF (1.8-fold; P = 0.049) and AR (1.2-fold; P = 0.07)
subjects. There were no changes in NAR subjects. On Sepharose-2B columns,
AB + m peaks were at 1.3-3.0 x 10(6) and 0.36-0.65 x 10(6) Da. 7F10-irm
showed four distinct peaks at 1.5, 1.2, 0.85, and 0.53 x 10(6) Da that were
nearly identical in both normal and CF samples. Sialic acid was present in
both 7F10-irm and AB + m. 7F10-irm and AB + m are mutually exclusive
sialylated mucoglycoproteins that are significantly induced in AR and CF
but not in NAR.
