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and surfactant
protein C mRNA in the MLE lung cell line
Departments of 1 Pediatrics and 2 Nutritional Sciences, Meriter Hospital Perinatal Center, University of Wisconsin, Madison, Wisconsin 53715
Lung development and surfactant biosynthesis are
affected by retinoic acid (RA) and dexamethasone (Dex). Using a mouse
lung epithelial cell line, we are exploring RA-Dex interactions through the study of RA and Dex effects on RA receptor (RAR) and surfactant protein (SP) C mRNA expression. RA increased expression of RAR-
(5.5 times) and SP-C (2 times) mRNA, with maximal effects at 24 h and at
10
6 M. The RA induction was not
inhibited by cycloheximide, suggesting RA affects transcription. With
added actinomycin D, RA did not affect the disappearance rate of
RAR- mRNA, but SP-C mRNA degradation was slowed, indicating an
effect on SP-C mRNA stability. Dex decreased RAR- and SP-C
expression to 75 and 70% of control values, respectively, with
greatest effects at 48 h and at
107 M. There was no effect
of Dex on either RAR- or SP-C mRNA disappearance with actinomycin D. However, cycloheximide prevented the effect of Dex. Despite Dex, RA
increased both RAR- and SP-C mRNA. This work suggests that RA and
Dex affect RAR- and SP-C genes by different mechanisms.
gene expression; type II cells; steroid hormone superfamily; lung development; vitamin A; messenger ribonucleic acid
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