AJP - Lung Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L26-L31, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jagielo, P. J.
Right arrow Articles by Schwartz, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jagielo, P. J.
Right arrow Articles by Schwartz, D. A.
Vol. 274, Issue 1, L26-L31, January 1998

Grain dust-induced lung inflammation is reduced by Rhodobacter sphaeroides diphosphoryl lipid A

Paul J. Jagielo1, Timothy J. Quinn1, Nilofer Qureshi2, and David A. Schwartz1

1 Department of Internal Medicine, The University of Iowa College of Medicine, Iowa City, Iowa 52242-1081; and 2 Department of Bacteriology, The University of Wisconsin, William S. Middleton Memorial Veteran's Hospital, Madison, Wisconsin 53705-2254

To further determine the importance of endotoxin in grain dust-induced inflammation of the lower respiratory tract, we evaluated the efficacy of pentaacylated diphosphoryl lipid A derived from the lipopolysaccharide of Rhodobacter sphaeroides (RsDPLA) as a partial agonist of grain dust-induced airway inflammation. RsDPLA is a relatively inactive compound compared with lipid A derived from Escherichia coli (LPS) and has been demonstrated to act as a partial agonist of LPS-induced inflammation. To assess the potential stimulatory effect of RsDPLA in relation to LPS, we incubated THP-1 cells with RsDPLA (0.001-100 µg/ml), LPS (0.02 µg endotoxin activity/ml), or corn dust extract (CDE; 0.02 µg endotoxin activity/ml). Incubation with RsDPLA revealed a tumor necrosis factor (TNF)-alpha stimulatory effect at 100 µg/ml. In contrast, incubation with LPS or CDE resulted in TNF-alpha release at 0.02 µg/ml. Pretreatment of THP-1 cells with varying concentrations of RsDPLA before incubation with LPS or CDE (0.02 µg endotoxin activity/ml) resulted in a dose-dependent reduction in the LPS- or CDE-induced release of TNF-alpha with concentrations of RsDPLA of up to 10 µg/ml but not at 100 µg/ml. To further understand the role of endotoxin in grain dust-induced airway inflammation, we utilized the unique LPS inhibitory property of RsDPLA to determine the inflammatory response to inhaled CDE in mice in the presence of RsDPLA. Ten micrograms of RsDPLA intratracheally did not cause a significant inflammatory response compared with intratracheal saline. However, pretreatment of mice with 10 µg of RsDPLA intratracheally before exposure to CDE (5.4 and 0.2 µg/m3) or LPS (7.2 and 0.28 µg/m3) resulted in significant reductions in the lung lavage concentrations of total cells, neutrophils, and specific proinflammatory cytokines compared with mice pretreated with sterile saline. These results confirm the LPS-inhibitory effect of RsDPLA and support the role of endotoxin as the principal agent in grain dust causing airway inflammation.

endotoxin; lipopolysaccharide inhibition; asthma


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. G. Coffman, J. C. Brown, D. A. Johnson, N. Parthasarathy, R. B. D'Agostino Jr., M. O. Lively, X. Hua, S. L. Tilley, W. Muller-Esterl, M. C. Willingham, et al.
Cleavage of high-molecular-weight kininogen by elastase and tryptase is inhibited by ferritin
Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L505 - L515.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Brass, J. W. Hollingsworth, E. McElvania-Tekippe, S. Garantziotis, I. Hossain, and D. A. Schwartz
CD14 is an essential mediator of LPS-induced airway disease
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L77 - L83.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Brass, J. D. Savov, S. H. Gavett, N. Haykal-Coates, and D. A. Schwartz
Subchronic endotoxin inhalation causes persistent airway disease
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L755 - L761.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. A. Schwartz, W. J. Christ, S. R. Kleeberger, and C. L. Wohlford-Lenane
Inhibition of LPS-induced airway hyperresponsiveness and airway inflammation by LPS antagonists
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L771 - L778.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. L. S. George, H. Jin, C. L. Wohlford-Lenane, M. E. O'Neill, J. C. Phipps, P. O'Shaughnessy, J. N. Kline, P. S. Thorne, and D. A. Schwartz
Endotoxin responsiveness and subchronic grain dust-induced airway disease
Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L203 - L213.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. R. Strohmeier, J. H. Walsh, E. S. Klings, H. W. Farber, W. W. Cruikshank, D. M. Center, and M. J. Fenton
Lipopolysaccharide Binding Protein Potentiates Airway Reactivity in a Murine Model of Allergic Asthma
J. Immunol., February 1, 2001; 166(3): 2063 - 2070.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. J. Quinn, S. Taylor, C. L. Wohlford-Lenane, and D. A. Schwartz
IL-10 reduces grain dust-induced airway inflammation and airway hyperreactivity
J Appl Physiol, January 1, 2000; 88(1): 173 - 179.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. A. Schwartz, C. L. Wohlford-Lenane, T. J. Quinn, and A. M. Krieg
Bacterial DNA or Oligonucleotides Containing Unmethylated CpG Motifs Can Minimize Lipopolysaccharide-Induced Inflammation in the Lower Respiratory Tract Through an IL-12-Dependent Pathway
J. Immunol., July 1, 1999; 163(1): 224 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. L. Wohlford-Lenane, D. C. Deetz, and D. A. Schwartz.
Cytokine gene expression after inhalation of corn dust
Am J Physiol Lung Cell Mol Physiol, May 1, 1999; 276(5): L736 - L743.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online