AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L39-L46, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhao, Q.
Right arrow Articles by Leikauf, G. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, Q.
Right arrow Articles by Leikauf, G. D.
Vol. 274, Issue 1, L39-L46, January 1998

Chemokine regulation of ozone-induced neutrophil and monocyte inflammation

Qiyu Zhao1, Leigh G. Simpson1, Kevin E. Driscoll1,4, and George D. Leikauf1,2,3

Departments of 1 Environmental Health, 2 Molecular and Cellular Physiology, and 3 Medicine, University of Cincinnati, Cincinnati 45267; and 4 Human and Environmental Safety Division and Corporate Research Division, Procter and Gamble Company, Cincinnati, Ohio 45239

Pulmonary inflammation has been observed in humans and in many animal species after ozone exposure. Inflammatory cell accumulation involves local synthesis of chemokines, including neutrophil chemoattractants such as macrophage inflammatory protein-2 (MIP-2), and monocyte chemoattractants, such as monocyte chemoattractant protein-1 (MCP-1). To better understand the mechanism of ozone-induced inflammation, we exposed mice and rats to ozone for 3 h and measured MIP-2 and MCP-1 gene expression. In C57BL/6 mice, steady-state mRNA levels for MCP-1 in the lung increased at 0.6 parts/million (ppm) ozone and were maximal at 2.0 ppm ozone. After exposure to 2 ppm ozone, MIP-2 mRNA levels peaked at 4 h postexposure, whereas MCP-1 mRNA levels peaked at 24 h postexposure. Neutrophils and monocytes recovered in bronchoalveolar lavage fluid peaked at 24 and 72 h, respectively. The accumulation of monocytes was thus delayed relative to that of neutrophils, consistent with the sequential expression of the corresponding chemokines. The role of MCP-1 in monocyte accumulation was evaluated in greater detail in rats. Ozone caused an increase in monocyte chemotactic activity in bronchoalveolar fluid that was inhibited by an antibody directed against MCP-1. Ozone-induced MCP-1 mRNA levels were higher in lavage cells than in whole lung tissue, indicating that lavage cells are an important source of MCP-1. In these cells, nuclear factor-kappa B, a nuclear transcription factor implicated in MCP-1 gene regulation, was also activated 20-24 h after ozone exposure. These findings indicate that monocyte accumulation subsequent to acute lung injury can be mediated through MCP-1 and that nuclear factor-kappa B may play a role in ozone-induced MCP-1 gene expression.

air pollution; asthma; inflammatory mediators; ozone


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
R. A. Johnston, T. A. Theman, F. L. Lu, R. D. Terry, E. S. Williams, and S. A. Shore
Diet-induced obesity causes innate airway hyperresponsiveness to methacholine and enhances ozone-induced pulmonary inflammation
J Appl Physiol, June 1, 2008; 104(6): 1727 - 1735.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. E. Lang, E. S. Williams, J. P. Mizgerd, and S. A. Shore
Effect of obesity on pulmonary inflammation induced by acute ozone exposure: role of interleukin-6
Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L1013 - L1020.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Mita, M. Satoh, A. Shimada, M. Okajima, S. Azuma, J. S. Suzuki, K. Sakabe, S. Hara, and S. Himeno
Metallothionein is a crucial protective factor against Helicobacter pylori-induced gastric erosive lesions in a mouse model
Am J Physiol Gastrointest Liver Physiol, April 1, 2008; 294(4): G877 - G884.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. A. Johnston, I. N. Schwartzman, L. Flynt, and S. A. Shore
Role of interleukin-6 in murine airway responses to ozone
Am J Physiol Lung Cell Mol Physiol, February 1, 2005; 288(2): L390 - L397.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. A. Johnston, J. P. Mizgerd, and S. A. Shore
CXCR2 is essential for maximal neutrophil recruitment and methacholine responsiveness after ozone exposure
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L61 - L67.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Fakhrzadeh, J. D. Laskin, and D. L. Laskin
Ozone-induced production of nitric oxide and TNF-{alpha} and tissue injury are dependent on NF-{kappa}B p50
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L279 - L285.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. L. Wright, S. G. Farmer, and A. Churg
Synthetic Serine Elastase Inhibitor Reduces Cigarette Smoke-induced Emphysema in Guinea Pigs
Am. J. Respir. Crit. Care Med., October 1, 2002; 166(7): 954 - 960.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Churg, J. Dai, H. Tai, C. Xie, and J. L. Wright
Tumor Necrosis Factor-{alpha} Is Central to Acute Cigarette Smoke-induced Inflammation and Connective Tissue Breakdown
Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 849 - 854.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
S. A. Shore, R. A. Johnston, I. N. Schwartzman, D. Chism, and G. G. Krishna Murthy
Ozone-induced airway hyperresponsiveness is reduced in immature mice
J Appl Physiol, March 1, 2002; 92(3): 1019 - 1028.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Michalec, B. K. Choudhury, E. Postlethwait, J. S. Wild, R. Alam, M. Lett-Brown, and S. Sur
CCL7 and CXCL10 Orchestrate Oxidative Stress-Induced Neutrophilic Lung Inflammation
J. Immunol., January 15, 2002; 168(2): 846 - 852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. A. SHORE, I. N. SCHWARTZMAN, B. LE BLANC, G. G. KRISHNA MURTHY, and C. M. DOERSCHUK
Tumor Necrosis Factor Receptor 2 Contributes to Ozone-induced Airway Hyperresponsiveness in Mice
Am. J. Respir. Crit. Care Med., August 15, 2001; 164(4): 602 - 607.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. R. Kleeberger, S. P. M. Reddy, L.-Y. Zhang, H.-Y. Cho, and A. E. Jedlicka
Toll-like receptor 4 mediates ozone-induced murine lung hyperpermeability via inducible nitric oxide synthase
Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L326 - L333.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. G. Knott, P. R. Gater, P. J. Dunford, M. E. Fuentes, and C. P. Bertrand
Rapid Up-Regulation of CXC Chemokines in the Airways after Ag-Specific CD4+ T Cell Activation
J. Immunol., January 15, 2001; 166(2): 1233 - 1240.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. W. McGraw, S. L. Forbes, J. C. W. Mak, D. P. Witte, P. E. Carrigan, G. D. Leikauf, and S. B. Liggett
Transgenic overexpression of beta 2-adrenergic receptors in airway epithelial cells decreases bronchoconstriction
Am J Physiol Lung Cell Mol Physiol, August 1, 2000; 279(2): L379 - L389.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. R. Kleeberger, S. Reddy, L.-Y. Zhang, and A. E. Jedlicka
Genetic Susceptibility to Ozone-Induced Lung Hyperpermeability . Role of Toll-Like Receptor 4
Am. J. Respir. Cell Mol. Biol., May 1, 2000; 22(5): 620 - 627.
[Abstract] [Full Text]

Home page
 ChestHome page
J. W. Christman, R. T. Sadikot, and T. S. Blackwell
The Role of Nuclear Factor-{kappa} B in Pulmonary Diseases
Chest, May 1, 2000; 117(5): 1482 - 1487.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. T. Borchers, S. Wesselkamper, S. E. Wert, S. D. Shapiro, and G. D. Leikauf
Monocyte inflammation augments acrolein-induced Muc5ac expression in mouse lung
Am J Physiol Lung Cell Mol Physiol, September 1, 1999; 277(3): L489 - L497.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. J. Kenyon, A. van der Vliet, B. C. Schock, T. Okamoto, G. M. McGrew, and J. A. Last
Susceptibility to ozone-induced acute lung injury in iNOS-deficient mice
Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L540 - L545.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online