| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Pediatrics and 2 Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235-9063
Prostacyclin is a key mediator of pulmonary vascular and parenchymal function during late fetal and early postnatal life, and its synthesis in whole lung increases during that period. The rate-limiting enzyme in prostacyclin synthesis in the developing lung is cyclooxygenase (COX). We investigated the ontogeny and cellular localization of COX-1 (constitutive) and COX-2 (inducible) gene expression in lungs from late-gestation fetal lambs, 1-wk-old newborn lambs (NB1), and 1- to 4-mo-old newborn lambs (NB2). COX-1 mRNA abundance rose progressively from fetal to NB1 to NB2, increasing 12-fold overall. In parallel, immunoblot analysis revealed a progressive increase in COX-1 protein, rising fourfold from fetal lambs to NB2. COX-2 mRNA levels increased fivefold from fetal to NB1 but were similar in NB1 and NB2. However, COX-2 protein was not detectable by immunoblot analysis in any age group. Immunohistochemistry for COX-1 showed intense immunostaining in endothelial cells at all ages. COX-1 was also expressed in airway epithelium at all ages, with a greater number of epithelial cells staining positively in NB2 compared with fetal and NB1 groups. In addition, COX-1 was expressed in airway smooth muscle from NB1. COX-2 immunostaining was absent in all age groups. These findings indicate that there is differential expression of COX-1 and COX-2 in the developing lung and that the enzymes are expressed in a cell-specific manner. The developmental upregulation in COX-1 may optimize the capacity for prostaglandin-mediated vasodilation, bronchodilation, and surfactant synthesis in the newborn lung.
airway epithelium; airway smooth muscle; immunohistochemistry; prostacyclin; vascular endothelium
This article has been cited by other articles:
|
|
 |
|
  R. Goyal, K. D. Creel, E. Chavis, G. D. Smith, L. D. Longo, and S. M. Wilson Maturation of intracellular calcium homeostasis in sheep pulmonary arterial smooth muscle cells Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L905 - L914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, V. Pozzato, G. Turato, A. Madamanchi, T. M. Murphy, and P. Chitano Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L964 - L973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, P. Chitano, and T. M. Murphy Length oscillation induces force potentiation in infant guinea pig airway smooth muscle Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L909 - L915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fayon, E. Dumas De La Roque, P. Berger, H. Begueret, O. Ousova, M. Molimard, and R. Marthan Increased relaxation of immature airways to {beta}2-adrenoceptor agonists is related to attenuated expression of postjunctional smooth muscle muscarinic M2 receptors J Appl Physiol, April 1, 2005; 98(4): 1526 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Fike, M. R. Kaplowitz, Y. Zhang, and S. L. Pfister Cyclooxygenase-2 and an early stage of chronic hypoxia-induced pulmonary hypertension in newborn pigs J Appl Physiol, March 1, 2005; 98(3): 1111 - 1118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. G. Geary, J. N. Buchholz, and W. J. Pearce Maturation depresses mouse cerebrovascular tone through endothelium-dependent mechanisms Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R734 - R741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Davidge Prostaglandin H Synthase and Vascular Function Circ. Res., October 12, 2001; 89(8): 650 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Parker, S. Afshar, J. P. Kinsella, T. R. Grover, S. Gebb, M. Geraci, P. W. Shaul, C. M. T. Cryer, and S. H. Abman Effects of chronic estrogen-receptor blockade on ovine perinatal pulmonary circulation Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1005 - H1014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nishio, Y. Suzuki, K. Takeshita, T. Aoki, H. Kudo, N. Sato, K. Naoki, N. Miyao, M. Ishii, and K. Yamaguchi Effects of hypercapnia and hypocapnia on [Ca2+]i mobilization in human pulmonary artery endothelial cells J Appl Physiol, June 1, 2001; 90(6): 2094 - 2100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Kirsch, I. S. Yuhanna, Z. Chen, Z. German, T. S. Sherman, and P. W. Shaul Estrogen Acutely Stimulates Endothelial Nitric Oxide Synthase in H441 Human Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., April 1, 1999; 20(4): 658 - 666. [Abstract] [Full Text]
|
|
|
|
|
|
T. S. Sherman, Z. Chen, I. S. Yuhanna, K. S. Lau, L. R. Margraf, and P. W. Shaul Nitric oxide synthase isoform expression in the developing lung epithelium Am J Physiol Lung Cell Mol Physiol, February 1, 1999; 276(2): L383 - L390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Chitano, C. M. Cox, and T. M. Murphy Relaxation of guinea pig trachealis during electrical field stimulation increases with age J Appl Physiol, May 1, 2002; 92(5): 1835 - 1842. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
