AJP - Lung AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L320-L329, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allen, C. B.
Right arrow Articles by White, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allen, C. B.
Right arrow Articles by White, C. W.
Vol. 274, Issue 3, L320-L329, March 1998

Changes in pulmonary expression of hexokinase and glucose transporter mRNAs in rats adapted to hyperoxia

Corrie B. Allen, Xiao-Ling Guo, and Carl W. White

Department of Pediatrics, National Jewish Medical and Research Center, and University of Colorado Health Sciences Center, Denver, Colorado 80206

Impairment of lung aconitase activity, citric acid cycle, and mitochondrial respiration by hyperoxia necessitates the elevation of glycolysis for energy production and of pentose shunt activity for reducing equivalents. The molecular mechanisms that allow increased glucose utilization are unknown. Adult male and female rats were adapted to sublethal hyperoxia, equivalent to 83% oxygen at sea level, or air for 7 days. Lung RNA and protein increased in hyperoxia (197 and 57%, respectively), whereas total DNA was unchanged. In hyperoxia, lung total hexokinase (HK) activity increased threefold, and mRNAs for HK-II and -III were specifically upregulated. HK-I mRNA was unchanged. mRNAs for HK-II and -III gradually increased during the first 72 h in hyperoxia. HK-II mRNA was significantly elevated at 72 h, preceding changes in lung cell populations. Although virtually absent in air, HK-II activity was highly expressed in hyperoxia. Among lung glucose transporters, specific expression of mRNAs for GLUT-4 (insulin dependent) and sodium-glucose cotransporter-1 was decreased, whereas that for GLUT-1 was minimally changed. Adaptation to hyperoxia involves coordinated changes in gene expression for the proteins regulating pulmonary glucose transport.

messenger ribonucleic acid; lung; glycolysis; monosaccharide transport proteins; energy metabolism


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Taneja, P. E. Coy, I. Lee, J. M. Bryson, and R. B. Robey
Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activity and hexokinase II isoform abundance
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C548 - C557.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Ahmad, A. Ahmad, E. Gerasimovskaya, K. R. Stenmark, C. B. Allen, and C. W. White
Hypoxia Protects Human Lung Microvascular Endothelial and Epithelial-like Cells against Oxygen Toxicity: Role of Phosphatidylinositol 3-Kinase
Am. J. Respir. Cell Mol. Biol., February 1, 2003; 28(2): 179 - 187.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Ahmad, S. Ahmad, B. K. Schneider, C. B. Allen, L.-Y. Chang, and C. W. White
Elevated expression of hexokinase II protects human lung epithelial-like A549 cells against oxidative injury
Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L573 - L584.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. B. Robey, J. Ma, A. V. P. Santos, O. A. Noboa, P. E. Coy, and J. M. Bryson
Regulation of Mesangial Cell Hexokinase Activity and Expression by Heparin-binding Epidermal Growth Factor-like Growth Factor. EPIDERMAL GROWTH FACTORS AND PHORBOL ESTERS INCREASE GLUCOSE METABOLISM VIA A COMMON MECHANISM INVOLVING CLASSIC MITOGEN-ACTIVATED PROTEIN KINASE PATHWAY ACTIVATION AND INDUCTION OF HEXOKINASE II EXPRESSION
J. Biol. Chem., April 19, 2002; 277(17): 14370 - 14378.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Bryson, P. E. Coy, K. Gottlob, N. Hay, and R. B. Robey
Increased Hexokinase Activity, of Either Ectopic or Endogenous Origin, Protects Renal Epithelial Cells against Acute Oxidant-induced Cell Death
J. Biol. Chem., March 22, 2002; 277(13): 11392 - 11400.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Ahmad, C. W. White, L.-Y. Chang, B. K. Schneider, and C. B. Allen
Glutamine protects mitochondrial structure and function in oxygen toxicity
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L779 - L791.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. R. Riddle, A. Ahmad, S. Ahmad, S. S. Deeb, M. Malkki, B. K. Schneider, C. B. Allen, and C. W. White
Hypoxia induces hexokinase II gene expression in human lung cell line A549
Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L407 - L416.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Sebastian, J. A. White, and J. E. Wilson
Characterization of the Rat Type III Hexokinase Gene Promoter. A FUNCTIONAL OCTAMER 1 MOTIF IS CRITICAL FOR BASAL PROMOTER ACTIVITY
J. Biol. Chem., October 29, 1999; 274(44): 31700 - 31706.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. Borok, S. Mihyu, V. F. J. Fernandes, X.-L. Zhang, K.-J. Kim, and R. L. Lubman
KGF prevents hyperoxia-induced reduction of active ion transport in alveolar epithelial cells
Am J Physiol Cell Physiol, June 1, 1999; 276(6): C1352 - C1360.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. C. Das, X.-L. Guo, and C. W. White
Induction of thioredoxin and thioredoxin reductase gene expression in lungs of newborn primates by oxygen
Am J Physiol Lung Cell Mol Physiol, March 1, 1999; 276(3): L530 - L539.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online