AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L343-L350, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Acarregui, M. J.
Right arrow Articles by Snyder, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Acarregui, M. J.
Right arrow Articles by Snyder, J. M.
Vol. 274, Issue 3, L343-L350, March 1998

O2 regulates surfactant protein A mRNA transcription and stability in human fetal lung in vitro

Michael J. Acarregui1, Ashish R. Kumar2, Scott T. Penisten1, and Jeanne M. Snyder2

Departments of 1 Pediatrics and 2 Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa 52242

The effect of O2 on surfactant protein (SP) A mRNA transcription and half-life was determined in midtrimester human fetal lung tissue cultured in either 20 (control) or 70% O2. Incubation of tissues in 70% O2 resulted in a 133% increase in SP-A mRNA transcription rate compared with control tissues. The SP-A mRNA half-life was increased by 54% in lung tissues cultured in 70% O2 vs. control tissues. Western blot analysis indicated a threefold increase in SP-A in the 70% O2 condition, demonstrating that O2 regulation of SP-A mRNA levels results in corresponding changes in SP-A levels. Primer extension assays were performed to determine whether the observed increase in SP-A mRNA levels is secondary to the preferential expression of one of the human SP-A genes, SP-A1 or SP-A2. Transcripts of both the SP-A1 and SP-A2 genes were increased ~100% in tissues maintained in 70% O2 compared with control tissues. These data demonstrate that O2 regulates human SP-A mRNA levels by both transcriptional and posttranscriptional mechanisms. Furthermore, because there is no differential effect of O2 on the expression of SP-A1 vs. SP-A2 mRNA, the properties of these genes that mediate regulation by O2 must be conserved between the two genes.

oxygen; messenger ribonucleic acid


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. S. Phokela, S. Peleg, F. R. Moya, and J. L. Alcorn
Regulation of human pulmonary surfactant protein gene expression by 1{alpha},25-dihydroxyvitamin D3
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L617 - L626.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y.-S. Yang, M.-C. W. Yang, B. Wang, and J. C. Weissler
BR22, a Novel Protein, Interacts with Thyroid Transcription Factor-1 and Activates the Human Surfactant Protein B Promoter
Am. J. Respir. Cell Mol. Biol., January 1, 2001; 24(1): 30 - 37.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. D. Bruno, J. A. Whitsett, G. F. Ross, and T. R. Korfhagen
Transcriptional Regulation of the Murine Surfactant Protein-A Gene by B-Myb
J. Biol. Chem., September 24, 1999; 274(39): 27523 - 27528.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online