AJP - Lung AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L369-L377, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guo, Y.
Right arrow Articles by Matalon, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, Y.
Right arrow Articles by Matalon, S.
Vol. 274, Issue 3, L369-L377, March 1998

Nitric oxide inhibits Na+ absorption across cultured alveolar type II monolayers

Yi Guo1, Michael D. Duvall1, John P. Crow1, and Sadis Matalon1,2,3

Departments of 1 Anesthesiology, 2 Physiology and Biophysics, and 3 Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama 35294

We examined the mechanisms by which nitric oxide (· NO) decreased vectorial Na+ transport across confluent monolayers of rat alveolar type II (ATII) cells grown on permeable supports. Amiloride (10 µM) applied to the apical side of monolayers inhibited ~90% of the equivalent (Ieq) and the short-circuit (Isc) current, with an half-maximal inhibitory concentration (IC50) of 0.85 µM, indicating that Na+ entry into ATII cells occurred through amiloride-sensitive Na+ channels. · NO generated by spermine NONOate and papa NONOate added to both sides of the monolayers decreased Ieq and increased transepithelial resistance in a concentration-dependent fashion (IC50 = 0.4 µM · NO). These changes were prevented or reversed by addition of oxyhemoglobin (50 µM). Incubation of ATII monolayers with 8-bromoguanosine 3',5'-cyclic monophosphate (400 µM) had no effect on transepithelial Na+ transport. When the basolateral membranes of ATII cells were permeabilized with amphotericin B (10 µM) in the presence of a mucosal-to-serosal Na+ gradient (145:25 mM), · NO (generated by 100 µM papa NONOate) inhibited ~60% of the amiloride-sensitive Isc. In addition, after permeabilization of the apical membranes, · NO inhibited the Isc [a measure of Na+-K+-adenosinetriphosphatase (ATPase) activity] by ~60%. We concluded that · NO at noncytotoxic concentrations decreased Na+ absorption across cultured ATII monolayers by inhibiting both the amiloride-sensitive Na+ channels and Na+-K+-ATPase through guanosine 3',5'-cyclic monophosphate-independent mechanisms.

ion channels; amiloride; N-ethyl-N-isopropyl amiloride; short-circuit current; sodium ion-potassium ion-adenosinetriphosphatase; oxyhemoglobin; guanosine 3',5'-cyclic monophosphate; amphotericin B; active transport


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. O'Brodovich, P. Yang, S. Gandhi, and G. Otulakowski
Amiloride-insensitive Na+ and fluid absorption in the mammalian distal lung
Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L401 - L408.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. M. Kaestle, C. A. Reich, N. Yin, H. Habazettl, J. Weimann, and W. M. Kuebler
Nitric oxide-dependent inhibition of alveolar fluid clearance in hydrostatic lung edema
Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L859 - L869.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Song and S. Matalon
Modulation of alveolar fluid clearance by reactive oxygen-nitrogen intermediates
Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L855 - L858.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Eisenhut
Effects of HSP70.1/3 gene knockout on NF-{kappa}B-mediated and cytokine-induced reduction in alveolar ion and fluid transport
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L365 - L365.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. N. Helms, L. Yu, B. Malik, D. J. Kleinhenz, C. M. Hart, and D. C. Eaton
Role of SGK1 in nitric oxide inhibition of ENaC in Na+-transporting epithelia
Am J Physiol Cell Physiol, September 1, 2005; 289(3): C717 - C726.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Swystun, L. Chen, P. Factor, B. Siroky, P. D. Bell, and S. Matalon
Apical trypsin increases ion transport and resistance by a phospholipase C-dependent rise of Ca2+
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L820 - L830.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K.-J. Kim, Z. Borok, C. Ehrhardt, B. C. Willis, C.-M. Lehr, and E. D. Crandall
Estimation of paracellular conductance of primary rat alveolar epithelial cell monolayers
J Appl Physiol, January 1, 2005; 98(1): 138 - 143.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
K. Kunzelmann and B. McMorran
First Encounter: How Pathogens Compromise Epithelial Transport
Physiology, October 1, 2004; 19(5): 240 - 244.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Sakuma, Y. Zhao, M. Sugita, M. Sagawa, H. Toga, T. Ishibashi, M. Nishio, and M. A. Matthay
Malnutrition impairs alveolar fluid clearance in rat lungs
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1268 - L1274.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, R. Frechette, Y. Yamagata, T. Yamagata, J.-F. Carmel, M.-E. Clermont, E. Brochiero, C. Masse, and Y. Berthiaume
Downregulation of ENaC activity and expression by TNF-{alpha} in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L301 - L311.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Matalon, K. M. Hardiman, L. Jain, D. C. Eaton, M. Kotlikoff, J. P. Eu, J. Sun, G. Meissner, and J. S. Stamler
Regulation of ion channel structure and function by reactive oxygen-nitrogen species
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1184 - L1189.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Lazrak and S. Matalon
cAMP-induced changes of apical membrane potentials of confluent H441 monolayers
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L443 - L450.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Frank, J.-F. Pittet, H. Lee, M. Godzich, and M. A. Matthay
High tidal volume ventilation induces NOS2 and impairs cAMP- dependent air space fluid clearance
Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L791 - L798.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
U. H. Thome, I. C. Davis, S. V. Nguyen, B. J. Shelton, and S. Matalon
Modulation of sodium transport in fetal alveolar epithelial cells by oxygen and corticosterone
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L376 - L385.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. Tsubochi, S. Suzuki, H. Kubo, T. Ueno, T. Yoshimura, T. Suzuki, H. Sasano, and T. Kondo
Early Changes in Alveolar Fluid Clearance by Nitric Oxide after Endotoxin Instillation in Rats
Am. J. Respir. Crit. Care Med., January 15, 2003; 167(2): 205 - 210.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. A. Matthay, H. G. Folkesson, and C. Clerici
Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema
Physiol Rev, July 1, 2002; 82(3): 569 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. M. Hardiman, J. R. Lindsey, and S. Matalon
Lack of amiloride-sensitive transport across alveolar and respiratory epithelium of iNOS({-}/{-}) mice in vivo
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L722 - L731.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Duszyk
Regulation of anion secretion by nitric oxide in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L450 - L457.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Fukuda, C. Jayr, A. Lazrak, Y. Wang, R. Lucas, S. Matalon, and M. A. Matthay
Mechanisms of TNF-{alpha} stimulation of amiloride-sensitive sodium transport across alveolar epithelium
Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1258 - L1265.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. B. WARE and M. A. MATTHAY
Alveolar Fluid Clearance Is Impaired in the Majority of Patients with Acute Lung Injury and the Acute Respiratory Distress Syndrome
Am. J. Respir. Crit. Care Med., May 1, 2001; 163(6): 1376 - 1383.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. J. Cummings and H. Wang
Nitric oxide decreases lung liquid production via guanosine 3',5'-cyclic monophosphate
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L923 - L929.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. H. Hatzoglou, K. I. Gourgoulianis, and P. A. Molyvdas
Effects of SNP, ouabain, and amiloride on electrical potential profile of isolated sheep pleura
J Appl Physiol, April 1, 2001; 90(4): 1565 - 1569.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
U. Thome, L. Chen, P. Factor, V. Dumasius, B. Freeman, J. Iasha Sznajder, and S. Matalon
Na,K-ATPase Gene Transfer Mitigates an Oxidant-Induced Decrease of Active Sodium Transport in Rat Fetal ATII Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 245 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. ZHU, L. B. WARE, T. GEISER, M. A. MATTHAY, and S. MATALON
Increased Levels of Nitrate and Surfactant Protein A Nitration in the Pulmonary Edema Fluid of Patients with Acute Lung Injury
Am. J. Respir. Crit. Care Med., January 1, 2001; 163(1): 166 - 172.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. G. Therien and R. Blostein
Mechanisms of sodium pump regulation
Am J Physiol Cell Physiol, September 1, 2000; 279(3): C541 - C566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. J. V. Galietta, C. Folli, C. Marchetti, L. Romano, D. Carpani, M. Conese, and O. Zegarra-Moran
Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-gamma
Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1186 - L1194.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
V. G. NIELSEN, M. S. BAIRD, L. CHEN, and S. MATALON
DETANONOate, a Nitric Oxide Donor, Decreases Amiloride-sensitive Alveolar Fluid Clearance in Rabbits
Am. J. Respir. Crit. Care Med., April 1, 2000; 161(4): 1154 - 1160.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Lazrak, A. Samanta, and S. Matalon
Biophysical properties and molecular characterization of amiloride-sensitive sodium channels in A549 cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2000; 278(4): L848 - L857.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Modelska, M. A. Matthay, L. A. S. Brown, E. Deutch, L. N. Lu, and J. F. Pittet
Inhibition of beta -adrenergic-dependent alveolar epithelial clearance by oxidant mechanisms after hemorrhagic shock
Am J Physiol Lung Cell Mol Physiol, May 1, 1999; 276(5): L844 - L857.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. C. Kone and S. Higham
Nitric oxide inhibits transcription of the Na+-K+-ATPase alpha 1-subunit gene in an MTAL cell line
Am J Physiol Renal Physiol, April 1, 1999; 276(4): F614 - F621.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. L. Elmer, K. G. Brady, M. L. Drumm, and T. J. Kelley
Nitric oxide-mediated regulation of transepithelial sodium and chloride transport in murine nasal epithelium
Am J Physiol Lung Cell Mol Physiol, March 1, 1999; 276(3): L466 - L473.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. G. Nielsen, M. D. Duvall, M. S. Baird, and S. Matalon
cAMP activation of chloride and fluid secretion across the rabbit alveolar epithelium
Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1127 - L1133.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Mairbaurl, K. Mayer, K.-J. Kim, Z. Borok, P. Bartsch, and E. D. Crandall
Alveolar Epithelial Ion and Fluid Transport: Hypoxia decreases active Na transport across primary rat alveolar epithelial cell monolayers
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L659 - L665.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Lazrak, U. Thome, C. Myles, J. Ware, L. Chen, C. J. Venglarik, and S. Matalon
Alveolar Epithelial Ion and Fluid Transport: cAMP regulation of Cl- and HCO3- secretion across rat fetal distal lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L650 - L658.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online