| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institut National de la
Santé et de la Recherche Médicale (INSERM) Unité 460 and 4 Services de Pneumologie des
Hôpitaux Bichat et Beaujon et INSERM Unité 408,
In striated muscle, chronic increases in
workload result in changes in myosin phenotype. The aim of this study
was to determine whether such changes occur in the diaphragm of
patients with severe chronic obstructive pulmonary disease, a situation
characterized by a chronic increase in respiratory load and lung
volume. Diaphragm biopsies were obtained from 22 patients who underwent
thoracic surgery. Myosin was characterized with electrophoresis in
nondenaturing conditions, SDS-glycerol PAGE, and Western blotting with
monoclonal antibodies specific for slow and fast myosin heavy chain
(MHC) isoforms. Flow volume curves, total lung capacity, and functional residual capacity were measured before surgery in 20 patients. We found
that the human diaphragm is composed of at least four myosin isoforms,
one slow and three fast, resulting from the combination of three MHC
species. Chronic overload was associated with an increase in the slow
-MHC species at the expense of the fast species (-MHC, 78.2 ± 4.6 and 50.0 ± 6.5% in emphysematous and control patients,
respectively; P < 0.005). Linear
correlations were found between -MHC percentage and forced
expiratory volume in 1 s (r = 
0.52;
P < 0.02), total lung capacity
(r = 0.44;
P < 0.05), and functional residual
capacity (r = 0.65;
P < 0.003). The human adult
diaphragm is composed of a balanced proportion of slow and fast myosin
isoforms. In patients with chronic obstructive pulmonary disease, the
proportion of fast myosins decreases, whereas that of slow myosin
increases. This increase appears to be closely related to lung
hyperinflation and may reflect an adaptation of the diaphragm to the
new functional requirements.
human diaphragm; myosin electrophoresis; chronic obstructive pulmonary disease; lung distension
This article has been cited by other articles:
|
|
 |
|
  T. L. Clanton and S. Levine Respiratory muscle fiber remodeling in chronic hyperinflation: dysfunction or adaptation? J Appl Physiol, July 1, 2009; 107(1): 324 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Stubbings, A. J. Moore, M. Dusmet, P. Goldstraw, T. G. West, M. I. Polkey, and M. A. Ferenczi Physiological properties of human diaphragm muscle fibres and the effect of chronic obstructive pulmonary disease J. Physiol., May 15, 2008; 586(10): 2637 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levine, T. Nguyen, M. Friscia, J. Zhu, W. Szeto, J. C. Kucharczuk, B. A. Tikunov, N. A. Rubinstein, L. R. Kaiser, and J. B. Shrager Parasternal intercostal muscle remodeling in severe chronic obstructive pulmonary disease J Appl Physiol, November 1, 2006; 101(5): 1297 - 1302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Doucet, R. Debigare, D.R. Joanisse, C. Cote, P. LeBlanc, J. Gregoire, J. Deslauriers, R. Vaillancourt, and F. Maltais Adaptation of the diaphragm and the vastus lateralis in mild-to-moderate COPD Eur. Respir. J., December 1, 2004; 24(6): 971 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.I. Polkey and J. Moxham Improvement in volitional tests of muscle function alone may not be adequate evidence that inspiratory muscle training is effective Eur. Respir. J., January 1, 2004; 23(1): 5 - 6. [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ribera, B. N'Guessan, J. Zoll, D. Fortin, B. Serrurier, B. Mettauer, X. Bigard, R. Ventura-Clapier, and E. Lampert Mitochondrial Electron Transport Chain Function Is Enhanced in Inspiratory Muscles of Patients with Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., March 15, 2003; 167(6): 873 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levine, T. Nguyen, L. R. Kaiser, and J. B. Shrager Evaluating Respiratory Muscle Adaptations: A New Approach Am. J. Respir. Crit. Care Med., December 1, 2002; 166(11): 1418 - 1419. [Full Text] [PDF]
|
|
|
|
|
|
A. Ramirez-Sarmiento, M. Orozco-Levi, R. Guell, E. Barreiro, N. Hernandez, S. Mota, M. Sangenis, J. M. Broquetas, P. Casan, and J. Gea Inspiratory Muscle Training in Patients with Chronic Obstructive Pulmonary Disease: Structural Adaptation and Physiologic Outcomes Am. J. Respir. Crit. Care Med., December 1, 2002; 166(11): 1491 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levine, C. Gregory, T. Nguyen, J. Shrager, L. Kaiser, N. Rubinstein, and G. Dudley Bioenergetic adaptation of individual human diaphragmatic myofibers to severe COPD J Appl Physiol, March 1, 2002; 92(3): 1205 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Baldwin and F. Haddad Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Effects of different activity and inactivity paradigms on myosin heavy chain gene expression in striated muscle J Appl Physiol, January 1, 2001; 90(1): 345 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. JEFFERY MADOR, T. J. KUFEL, and L. A. PINEDA Quadriceps and Diaphragmatic Function after Exhaustive Cycle Exercise in the Healthy Elderly Am. J. Respir. Crit. Care Med., November 1, 2000; 162(5): 1760 - 1766. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. M A Heunks and P N R. Dekhuijzen Respiratory muscle function and free radicals: from cell to COPD Thorax, August 1, 2000; 55(8): 704 - 716. [Full Text]
|
|
|
|
|
|
T. Nguyen, J. Shrager, L. Kaiser, L. Mei, M. Daood, J. Watchko, N. Rubinstein, and S. Levine Developmental myosin heavy chains in the adult human diaphragm: coexpression patterns and effect of COPD J Appl Physiol, April 1, 2000; 88(4): 1446 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. JEFFERY MADOR, T. J. KUFEL, L. A. PINEDA, and G. K. SHARMA Diaphragmatic Fatigue and High-intensity Exercise in Patients with Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., January 1, 2000; 161(1): 118 - 123. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
