| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Division of Pulmonary and Critical Care Medicine, Department of Medicine, and Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina 27710
Heme oxygenase (HO)-1, which catalyzes heme breakdown, is induced by oxidative stress and may protect against oxidative injury. We hypothesized that induction of HO-1 by hemoglobin (Hb) in the lung would protect the rat from pulmonary O2 toxicity. Rats given intratracheal (IT) Hb showed lung-specific induction of HO-1 by 8 h by Western analysis. Rats were then pretreated for 8 h before 60 h of exposure to 100% O2 with either IT normal saline, Hb, or Hb plus the HO-1 inhibitor tin-protoporphyrin (SnPP). Both the Hb+O2 and Hb+O2+ SnPP animals had less lung injury than normal saline controls as indicated by lower pleural fluid volumes and wet-to-dry weight ratios (P < 0.01). The improvement in injury in the two Hb-treated groups was the same despite a 61% decrease in HO enzyme activity in the Hb+SnPP group after 60 h of O2. In addition, inhibition of HO activity with SnPP alone before O2 exposure did not augment the extent of hyperoxic lung injury. These results demonstrate that IT Hb induces lung HO-1 in the rat and protects against hyperoxia; however, the protection is not mediated by increased HO enzyme activity.
oxidative stress; reactive oxygen species; antioxidant enzymes
This article has been cited by other articles:
|
|
 |
|
  I. T. Demchenko, K. E. Welty-Wolf, B. W. Allen, and C. A. Piantadosi Similar but not the same: normobaric and hyperbaric pulmonary oxygen toxicity, the role of nitric oxide Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L229 - L238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. MAESTRELLI, A. H. EL MESSLEMANI, O. DE FINA, Y. NOWICKI, M. SAETTA, C. MAPP, and L. M. FABBRI Increased Expression of Heme Oxygenase (HO)-1 in Alveolar Spaces and HO-2 in Alveolar Walls of Smokers Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): 1508 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Clayton, M. S. Carraway, H. B. Suliman, E. D. Thalmann, K. N. Thalmann, D. E. Schmechel, and C. A. Piantadosi Inhaled carbon monoxide and hyperoxic lung injury in rats Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L949 - L957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Donnelly and P. J. Barnes Expression of Heme Oxygenase in Human Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 295 - 303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Otterbein and A. M. K. Choi Heme oxygenase: colors of defense against cellular stress Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1029 - L1037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. O'Donaughy and B. R. Walker Renal vasodilatory influence of endogenous carbon monoxide in chronically hypoxic rats Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H2908 - H2915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Marton, X. Wang, A. Kowalczuk, Z.-D. Zhang, E. Windmeyer, and R. L. Macdonald Effects of hemoglobin on heme oxygenase gene expression and viability of cultured smooth muscle cells Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2405 - H2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Weng, A. Tatarov, B. P. Bartos, C. H. Contag, and P. A. Dennery HO-1 expression in type II pneumocytes after transpulmonary gene delivery Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1273 - L1279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Balla, G. Balla, V. Jeney, G. Kakuk, H. S. Jacob, and G. M. Vercellotti Ferriporphyrins and endothelium: a 2-edged sword---promotion of oxidation and induction of cytoprotectants Blood, June 1, 2000; 95(11): 3442 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yamada, M. Yamaya, S. Okinaga, R. Lie, T. Suzuki, K. Nakayama, A. Takeda, T. Yamaguchi, Y. Itoyama, K. Sekizawa, et al. Protective Effects of Heme Oxygenase-1 against Oxidant-Induced Injury in the Cultured Human Tracheal Epithelium Am. J. Respir. Cell Mol. Biol., September 1, 1999; 21(3): 428 - 435. [Abstract] [Full Text]
|
|
|
|
|
|
L. E. Otterbein, L. L. Mantell, and A. M. K. Choi Carbon monoxide provides protection against hyperoxic lung injury Am J Physiol Lung Cell Mol Physiol, April 1, 1999; 276(4): L688 - L694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Carraway, A. J. Ghio, J. L. Taylor, and C. A. Piantadosi Induction of ferritin and heme oxygenase-1 by endotoxin in the lung Am J Physiol Lung Cell Mol Physiol, September 1, 1998; 275(3): L583 - L592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Carraway, A. J. Ghio, H. B. Suliman, J. D. Carter, A. R. Whorton, and C. A. Piantadosi Carbon monoxide promotes hypoxic pulmonary vascular remodeling Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L693 - L702. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
