| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; and 2 Department of Chemistry and Biochemistry, Newark, Delaware 19716
A clone for a rat acidic Ca2+-independent phospholipase A2 (aiPLA2) was isolated from a cDNA library prepared from rat granular pneumocytes with a probe based on the human aiPLA2 sequence (T. S. Kim, C. S. Sundaresh, S. I. Feinstein, C. Dodia, W. R. Skach, M. K. Jain, T. Nagase, N. Seki, K. Ishikawa, N. Nomura, and A. B. Fisher. J. Biol. Chem. 272: 2542-2550, 1997). In addition, a consensus sequence for mouse aiPLA2 was constructed from several mouse cDNA clones in the GenBank and dbEST databases. Each sequence codes for a 224-amino acid protein with 88% identity of the amino acids among the three species and conservation of a putative lipase motif (GDSWG). Translation of mRNA produced from the rat clone in a wheat germ system resulted in expression of PLA2 activity with properties similar to those of the human enzyme, i.e., acidic pH optimum and Ca2+ independence. The localization of aiPLA2 in rat tissues was studied with the human cDNA probe, polyclonal and monoclonal antibodies, and aiPLA2 activity. aiPLA2 is present in the lung as evidenced by high levels of mRNA and protein expression and by enzymatic activity that is inhibited by anti-PLA2 antibody and by the transition state analog 1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol (MJ33). Immunocytochemistry showed the presence of aiPLA2 in alveolar type II cells, alveolar macrophages, and bronchiolar epithelium. In the brain, heart, liver, kidney, spleen, and intestine, aiPLA2 mRNA content was <50% of that in the lung, immunoreactive protein was not detectable, and enzymatic activity was not inhibited by MJ33 or aiPLA2 antibody. These results show marked enrichment of aiPLA2 in the lung compared with the other organs and suggest translational control of aiPLA2 expression.
phospholipase A2; calcium ion; mouse; liposome; lipase; 1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol; active serine; in vitro translation; type II cell complementary deoxyribonucleic acid expression library
This article has been cited by other articles:
|
|
 |
|
  Y. Wang, Y. Manevich, S. I. Feinstein, and A. B. Fisher Adenovirus-mediated transfer of the 1-cys peroxiredoxin gene to mouse lung protects against hyperoxic injury Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1188 - L1193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-S. Kim, Y. Manevich, S. I. Feinstein, J. H. Pak, Y. S. Ho, and A. B. Fisher Induction of 1-cys peroxiredoxin expression by oxidative stress in lung epithelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L363 - L369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Guo, W. Su, Z. Ma, G. M. Smith, and M. C. Gong Ca2+-independent Phospholipase A2 Is Required for Agonist-induced Ca2+ Sensitization of Contraction in Vascular Smooth Muscle J. Biol. Chem., January 10, 2003; 278(3): 1856 - 1863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Pak, Y. Manevich, H. S. Kim, S. I. Feinstein, and A. B. Fisher An Antisense Oligonucleotide to 1-cys Peroxiredoxin Causes Lipid Peroxidation and Apoptosis in Lung Epithelial Cells J. Biol. Chem., December 13, 2002; 277(51): 49927 - 49934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. T. Power, J. M. Shannon, P. C. Blumbergs, and W.-P. Gai Nonselenium Glutathione Peroxidase in Human Brain : Elevated Levels in Parkinson's Disease and Dementia with Lewy Bodies Am. J. Pathol., September 1, 2002; 161(3): 885 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Fisher and C. Dodia Lysosomal-type PLA2 and turnover of alveolar DPPC Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L748 - L754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yano, R. J. Mason, T. Pan, R. R. Deterding, L. D. Nielsen, and J. M. Shannon KGF regulates pulmonary epithelial proliferation and surfactant protein gene expression in adult rat lung Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1146 - L1158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Quintero and J. R. Wright Metabolism of phosphatidylglycerol by alveolar macrophages in vitro Am J Physiol Lung Cell Mol Physiol, August 1, 2000; 279(2): L399 - L407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tan and S. C. Hooi Syncollin is differentially expressed in rat proximal small intestine and regulated by feeding behavior Am J Physiol Gastrointest Liver Physiol, February 1, 2000; 278(2): G308 - G320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. Tithof, J. Olivero, K. Ruehle, and P. E. Ganey Activation of Neutrophil Calcium-Dependent and -Independent Phospholipases A2 by Organochlorine Compounds Toxicol. Sci., January 1, 2000; 53(1): 40 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Fisher, C. Dodia, Y. Manevich, J.-W. Chen, and S. I. Feinstein Phospholipid Hydroperoxides Are Substrates for Non-selenium Glutathione Peroxidase J. Biol. Chem., July 23, 1999; 274(30): 21326 - 21334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-W. Chen, C. Dodia, S. I. Feinstein, M. K. Jain, and A. B. Fisher 1-Cys Peroxiredoxin, a Bifunctional Enzyme with Glutathione Peroxidase and Phospholipase A2 Activities J. Biol. Chem., September 8, 2000; 275(37): 28421 - 28427. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
