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Am J Physiol Lung Cell Mol Physiol 274: L786-L792, 1998;
1040-0605/98 $5.00
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Vol. 274, Issue 5, L786-L792, May 1998

Smooth muscle actin and myosin expression in cultured airway smooth muscle cells

Jean Z. Wong1, Janet Woodcock-Mitchell1, John Mitchell1, Patricia Rippetoe1, Sheryl White1, Marlene Absher1, Linda Baldor1, John Evans1, Kirk M. McHugh2, and Robert B. Low1

1 Departments of Molecular Physiology and Biophysics and of Medicine, University of Vermont, Burlington, Vermont 05405; and 2 Department of Anatomy and Developmental Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

In this study, the expression of smooth muscle actin and myosin was examined in cultures of rat tracheal smooth muscle cells. Protein and mRNA analyses demonstrated that these cells express alpha - and gamma -smooth muscle actin and smooth muscle myosin and nonmuscle myosin-B heavy chains. The expression of the smooth muscle specific actin and myosin isoforms was regulated in the same direction when growth conditions were changed. Thus, at confluency in 1 or 10% serum-containing medium as well as for low-density cells (50-60% confluent) deprived of serum, the expression of the smooth muscle forms of actin and myosin was relatively high. Conversely, in rapidly proliferating cultures at low density in 10% serum, smooth muscle contractile protein expression was low. The expression of nonmuscle myosin-B mRNA and protein was more stable and was upregulated only to a small degree in growing cells. Our results provide new insight into the molecular basis of differentiation and contractile function in airway smooth muscle cells.

growth state


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