| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Pharmacology and 2 Pathology, University of South Alabama College of Medicine, Mobile, Alabama 36688
Cytosolic Ca2+ concentration ([Ca2+]i) plays an important role in control of pulmonary vascular endothelial cell (ECs) barrier function. In this study, we investigated whether thapsigargin- and ionomycin-induced changes in cytosolic Ca2+ induce permeability in rat pulmonary microvascular (RPMV) versus macrovascular (RPA) ECs. In Transwell cultures, RPMVECs formed a tighter, more restrictive barrier than RPAECs to 12,000-, 72,000-, and 150,000-molecular-weight FITC-labeled dextrans. Thapsigargin (1 µM) produced higher [Ca2+]i levels in RPAECs than in RPMVECs and increased permeability in RPAEC but not in RPMVEC monolayers. Due to the attenuated [Ca2+]i response in RPMVECs, we investigated whether reduced activation of store-operated Ca2+ entry was responsible for the insensitivity to thapsigargin. Addition of the drug in media containing 100 nM extracellular Ca2+ followed by readdition media with 2 mM extracellular Ca2+ increased RPMVEC [Ca2+]i to a level higher than that in RPAECs. Under these conditions, RPMVEC permeability was not increased, suggesting that [Ca2+]i in RPMVECs does not initiate barrier disruption. Also, ionomycin (1.4 µM) did not alter RPMVEC permeability, but the protein phosphatase inhibitor calyculin A (100 nM) induced permeability in RPMVECs. These data indicate that, whereas increased [Ca2+]i promotes permeability in RPAECs, it is not sufficient in RPMVECs, which show an apparent uncoupling of [Ca2+]i signaling pathways or dominant Ca2+-independent mechanisms from controlling cellular gap formation and permeability.
microvasculature; fluorescein isothiocyanate-labeled dextran; gap formation; thapsigargin; ionomycin
This article has been cited by other articles:
|
|
 |
|
  R. Chang, L. G. Chicoine, H. Cui, N. L. Kanagy, B. R. Walker, Y. Liu, B. K. English, and L. D. Nelin Cytokine-induced arginase activity in pulmonary endothelial cells is dependent on Src family tyrosine kinase activity Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L688 - L697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Clark, D. F. Alvarez, M. Alexeyev, J. A. C. King, L. Huang, M. C. Yoder, and T. Stevens Regulatory role for nucleosome assembly protein-1 in the proliferative and vasculogenic phenotype of pulmonary endothelium Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L431 - L439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Alvarez, L. Huang, J. A. King, M. K. ElZarrad, M. C. Yoder, and T. Stevens Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L419 - L430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Solodushko and B. Fouty Proproliferative phenotype of pulmonary microvascular endothelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L671 - L677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Parker Hydraulic conductance of lung endothelial phenotypes and Starling safety factors against edema Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L378 - L380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Parker, T. Stevens, J. Randall, D. S. Weber, and J. A. King Hydraulic conductance of pulmonary microvascular and macrovascular endothelial cell monolayers Am J Physiol Lung Cell Mol Physiol, July 1, 2006; 291(1): L30 - L37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhu, S. Strada, and T. Stevens Cyclic GMP-specific phosphodiesterase 5 regulates growth and apoptosis in pulmonary endothelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L196 - L206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Irwin, M. C. Tissot van Patot, A. Tucker, and R. Bowen Direct ANP inhibition of hypoxia-induced inflammatory pathways in pulmonary microvascular and macrovascular endothelial monolayers Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L849 - L859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, Z. Ma, Z.-L. Tang, T. Stevens, B. Pitt, and S. Li CpG DNA-mediated immune response in pulmonary endothelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L552 - L558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Parker and M. I. Townsley Evaluation of lung injury in rats and mice Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L231 - L246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. V. Kim, F. Di Cello, C. S. Hillaire, and K. S. Kim Differential Ca2+ signaling by thrombin and protease-activated receptor-1-activating peptide in human brain microvascular endothelial cells Am J Physiol Cell Physiol, January 1, 2004; 286(1): C31 - C42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Usatyuk, V. P. Fomin, S. Shi, J. G. N. Garcia, K. Schaphorst, and V. Natarajan Role of Ca2+ in diperoxovanadate-induced cytoskeletal remodeling and endothelial cell barrier function Am J Physiol Lung Cell Mol Physiol, November 1, 2003; 285(5): L1006 - L1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Tarpey, D. R. Sawmiller, C. Kelly, W. J. Thompson, and M. I. Townsley Phosphodiesterase 3 activity is reduced in dog lung following pacing-induced heart failure Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L766 - L773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Siflinger-Birnboim and A. Johnson Protein kinase C modulates pulmonary endothelial permeability: a paradigm for acute lung injury Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L435 - L451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Creighton, N. Masada, D. M. F. Cooper, and T. Stevens Coordinate regulation of membrane cAMP by Ca2+-inhibited adenylyl cyclase and phosphodiesterase activities Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L100 - L107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Minnear Vascular endothelial cells actively participate in high inflation pressure-induced permeability and edema Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1200 - L1202. [Full Text] [PDF]
|
|
|
|
|
|
J. C. Parker and S. Yoshikawa Vascular segmental permeabilities at high peak inflation pressure in isolated rat lungs Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1203 - L1209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wang, G. R. Pfeiffer II, T. Stevens, and C. M. Doerschuk Lung Microvascular and Arterial Endothelial Cells Differ in Their Responses to Intercellular Adhesion Molecule-1 Ligation Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 872 - 877. [Abstract] [Full Text]
|
|
|
|
|
|
T. Stevens Bronchial endothelial cell phenotypes and the form:function relationship Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L518 - L519. [Full Text] [PDF]
|
|
|
|
|
|
A. Moldobaeva and E. M. Wagner Heterogeneity of bronchial endothelial cell permeability Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L520 - L527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Stevens, R. Rosenberg, W. Aird, T. Quertermous, F. L. Johnson, J. G. N. Garcia, R. P. Hebbel, R. M. Tuder, and S. Garfinkel NHLBI workshop report: endothelial cell phenotypes in heart, lung, and blood diseases Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1422 - C1433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Dudek and J. G. N. Garcia Cytoskeletal regulation of pulmonary vascular permeability J Appl Physiol, October 1, 2001; 91(4): 1487 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H. BROUGH, S. WU, D. CIOFFI, T. M. MOORE, M. LI, N. DEAN, and T. STEVENS Contribution of endogenously expressed Trp1 to a Ca2+-selective, store-operated Ca2+ entry pathway FASEB J, August 1, 2001; 15(10): 1727 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Grishko, M. Solomon, G. L. Wilson, S. P. LeDoux, and M. N. Gillespie Oxygen radical-induced mitochondrial DNA damage and repair in pulmonary vascular endothelial cell phenotypes Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1300 - L1308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lum and K. A. Roebuck Oxidant stress and endothelial cell dysfunction Am J Physiol Cell Physiol, April 1, 2001; 280(4): C719 - C741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Maron, Z. Fu, O. Mathieu-Costello, and J. B. West Effect of high transcapillary pressures on capillary ultrastructure and permeability coefficients in dog lung J Appl Physiol, February 1, 2001; 90(2): 638 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wang, E. T. Chiang, M. Lim, J. Lai, R. Rogers, P. A. Janmey, D. Shepro, and C. M. Doerschuk Changes in the biomechanical properties of neutrophils and endothelial cells during adhesion Blood, February 1, 2001; 97(3): 660 - 668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Greenstein, J. Barnard, K. Zhou, M. Fong, and B. Hendey Fas activation reduces neutrophil adhesion to endothelial cells J. Leukoc. Biol., November 1, 2000; 68(5): 715 - 722. [Abstract] [Full Text]
|
|
|
|
|
|
N. Norwood, T. M. Moore, D. A. Dean, R. Bhattacharjee, M. Li, and T. Stevens Store-operated calcium entry and increased endothelial cell permeability Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L815 - L824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Moore, N. R. Norwood, J. R. Creighton, P. Babal, G. H. Brough, D. M. Shasby, and T. Stevens Receptor-dependent activation of store-operated calcium entry increases endothelial cell permeability Am J Physiol Lung Cell Mol Physiol, October 1, 2000; 279(4): L691 - L698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Stevens, J. Creighton, and W. J. Thompson Control of cAMP in lung endothelial cell phenotypes. Implications for control of barrier function Am J Physiol Lung Cell Mol Physiol, July 1, 1999; 277(1): L119 - L126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Chetham, P. Babal, J. P. Bridges, T. M. Moore, and T. Stevens Segmental regulation of pulmonary vascular permeability by store-operated Ca2+ entry Am J Physiol Lung Cell Mol Physiol, January 1, 1999; 276(1): L41 - L50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Moore, G. H. Brough, P. Babal, J. J. Kelly, M. Li, and T. Stevens Store-operated calcium entry promotes shape change in pulmonary endothelial cells expressing Trp1 Am J Physiol Lung Cell Mol Physiol, September 1, 1998; 275(3): L574 - L582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Moore, P. M. Chetham, J. J. Kelly, and T. Stevens Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP Am J Physiol Lung Cell Mol Physiol, August 1, 1998; 275(2): L203 - L222. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
