AJP - Lung Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L864-L869, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yao, X. L.
Right arrow Articles by Shelhamer, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yao, X. L.
Right arrow Articles by Shelhamer, J. H.
Vol. 274, Issue 5, L864-L869, May 1998

Interferon-gamma stimulates human Clara cell secretory protein production by human airway epithelial cells

X. L. Yao, T. Ikezono, M. Cowan, C. Logun, C. W. Angus, and J. H. Shelhamer

Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892

Clara cell secretory protein (CCSP) is an inhibitor of secretory phospholipase A2. It is produced by airway epithelial cells and is present in airway secretions. Because interferon (IFN)-gamma can induce gene expression in airway epithelial cells and may modulate the inflammatory response in the airway, it was of interest to study the effect of this cytokine on epithelial cell CCSP mRNA expression and CCSP protein synthesis. A human bronchial epithelial cell line (BEAS-2B) was used for this study. CCSP mRNA was detected by ribonuclease protection assay. IFN-gamma was found to increase CCSP mRNA expression in a time- and dose-dependent manner. The CCSP mRNA level increased after IFN-gamma (300 U/ml) treatment for 8-36 h, with the peak increase at 18 h. Immunobloting of CCSP protein also demonstrated that IFN-gamma induced the synthesis and secretion of CCSP protein in a time-dependent manner. Nuclear run-on, CCSP reporter gene activity assay, and CCSP mRNA half-life assay demonstrated that IFN-gamma -induced increases in CCSP gene expression were mediated, at least in part, at the posttranscriptional level. The present study demonstrates that IFN-gamma can induce increases in steady-state mRNA levels and protein synthesis of human CCSP protein in airway epithelial cells and may modulate airway inflammatory responses in this manner.

cytokines; airway inflammation; airway secretion


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. L. Ramsay, Z. Luo, S. M. Magdaleno, S. K. Whitbourne, X. Cao, M. S. Park, S. E. Welty, L.-Y. Yu-Lee, and F. J. DeMayo
Transcriptional regulation of CCSP by interferon-gamma in vitro and in vivo
Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L108 - L118.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. M. Watson, S. D. Reynolds, G. W. Mango, I.-M. Boe, J. Lund, and B. R. Stripp
Altered lung gene expression in CCSP-null mice suggests immunoregulatory roles for Clara cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1523 - L1530.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Hayashida, K. S. Harrod, and J. A. Whitsett
Regulation and function of CCSP during pulmonary Pseudomonas aeruginosa infection in vivo
Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L452 - L459.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Condino-Neto and P. E. Newburger
Interferon-gamma improves splicing efficiency of CYBB gene transcripts in an interferon-responsive variant of chronic granulomatous disease due to a splice site consensus region mutation
Blood, June 1, 2000; 95(11): 3548 - 3554.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online