AJP - Lung Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L1006-L1016, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Postlethwait, E. M.
Right arrow Articles by Pryor, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Postlethwait, E. M.
Right arrow Articles by Pryor, W. A.
Vol. 274, Issue 6, L1006-L1016, June 1998

O3-induced formation of bioactive lipids: estimated surface concentrations and lining layer effects

Edward M. Postlethwait1, Rafael Cueto2, Leonard W. Velsor1, and William A. Pryor2

1 Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas 77555-0876; and 2 Biodynamics Institute, Louisiana State University, Baton Rouge, Louisiana 70803-1800

Recent evidence suggests that inhaled ozone (O3) does not induce toxicity via direct epithelial interactions. Reactions with epithelial lining fluid (ELF) constituents limit cellular contact and generate products, including lipid ozonation products, postulated to initiate pathophysiological cascades. To delineate specific aspects of lipid ozonation product formation and to estimate in situ surface concentrations, we studied the O3 absorption characteristics of ELF constituent mixtures and measured hexanal, heptanal, and nonanal yields as a function of ascorbic acid (AH2) concentration. Exposures of isolated rat lungs, bronchoalveolar lavage fluid (BALF) and egg phosphatidylcholine (PC) liposomes were conducted. 1) O3 absorption by AH2, uric acid, and albumin exceeded that by egg PC and glutathione. O3 reaction with egg PC occurred when AH2 concentrations were reduced. 2) Aldehydes were produced in low yield during lung and BALF exposures in a time- and O3 concentration-dependent manner. 3) Diminishing BALF AH2 content lowered O3 uptake but increased aldehyde yields. Conversely, AH2 addition to egg PC increased O3 uptake but reduced aldehyde yields. Estimations of bioactive ozonation and autoxidation product accumulation within the ELF suggested possible nanomolar to low micromolar concentrations. The use of reaction products as metrics of O3 exposure may have intrinsic sensitivity and specificity limitations. Moreover, due to the heterogenous nature of O3 reactions within the ELF, dose-response relationships may not be linear with respect to O3 absorption.

ozone; pulmonary oxidant stress; epithelial lining fluid; lipid oxidation; aldehydes; ozonation products; ascorbic acid


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Matsubara, K. Takeda, N. Jin, M. Okamoto, H. Matsuda, Y. Shiraishi, J. W. Park, G. McConville, A. Joetham, R. L. O'Brien, et al.
V{gamma}1+ T Cells and Tumor Necrosis Factor-Alpha in Ozone-Induced Airway Hyperresponsiveness
Am. J. Respir. Cell Mol. Biol., April 1, 2009; 40(4): 454 - 463.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. S. Schelegle, W. F. Walby, and W. C. Adams
Time course of ozone-induced changes in breathing pattern in healthy exercising humans
J Appl Physiol, February 1, 2007; 102(2): 688 - 697.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. K. Pulfer, C. Taube, E. Gelfand, and R. C. Murphy
Ozone Exposure in Vivo and Formation of Biologically Active Oxysterols in the Lung
J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 256 - 264.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. M. Connor, C. A. Ballinger, T. B. Albrecht, and E. M. Postlethwait
Interfacial phospholipids inhibit ozone-reactive absorption-mediated cytotoxicity in vitro
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1169 - L1178.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. C. Long, J. Suh, J. D. Morrow, R. H. Schiestl, G. G. K. Murthy, J. D. Brain, and B. Frei
Ozone causes lipid peroxidation but little antioxidant depletion in exercising and nonexercising hamsters
J Appl Physiol, October 1, 2001; 91(4): 1694 - 1700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
E. M. Postlethwait, J. P. Joad, D. M. Hyde, E. S. Schelegle, J. M. Bric, A. J. Weir, L. F. Putney, V. J. Wong, L. W. Velsor, and C. G. Plopper
Three-Dimensional Mapping of Ozone-Induced Acute Cytotoxicity in Tracheobronchial Airways of Isolated Perfused Rat Lung
Am. J. Respir. Cell Mol. Biol., February 1, 2000; 22(2): 191 - 199.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. M. KAFOURY, W. A. PRYOR, G. L. SQUADRITO, M. G. SALGO, X. ZOU, and M. FRIEDMAN
Induction of Inflammatory Mediators in Human Airway Epithelial Cells by Lipid Ozonation Products
Am. J. Respir. Crit. Care Med., December 1, 1999; 160(6): 1934 - 1942.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online