AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 274: L1066-L1072, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruddy, M. K.
Right arrow Articles by Harris, H. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruddy, M. K.
Right arrow Articles by Harris, H. W.
Vol. 274, Issue 6, L1066-L1072, June 1998

Modulation of aquaporin 4 and the amiloride-inhibitable sodium channel in perinatal rat lung epithelial cells

M. K. Ruddy1,2, J. M. Drazen1, O. M. Pitkanen3,4, B. Rafii3, H. M. O'Brodovich3, and H. W. Harris2

1 Division of Respiratory Diseases, Brigham and Women's Hospital, and 2 Division of Nephrology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; 3 Respiratory Research and Medical Research Council Group on Lung Development, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada M5G 1X8; and 4 Children's Hospital, University of Helsinki, FIN-00290 Helsinki, Finland

During the perinatal period, a dramatic reversal of lung transepithelial ion and water transport occurs that involves the amiloride-inhibitable Na+ channel (ENaC). Aquaporin (AQP) water channel proteins facilitate cell membrane water transport. We now report that AQP-4, localized to basolateral membranes of airway epithelial cells, increases its mRNA expression in developing lung eightfold during the 2 days before birth to reach a peak on the first postnatal day in the lungs but not in brains or kidneys of neonatal rats. AQP-4 and the alpha -, beta -, and gamma -subunits of ENaC are both expressed by cultured rat fetal distal lung epithelial (FDLE) cells. AQP-4 and ENaC expression increase in FDLE cells cultured on uncoated permeant filters compared with matched control cells cultured on filters containing extracellular matrix derived from fetal lung epithelial cells. Similarly, AQP-4 expression increases in FDLE cells exposed to 21% O2 compared with cells exposed to 3% O2. These data demonstrate that AQP-4 expression is highest on the first day after birth in neonatal rat lungs. Exposure to ambient 21% O2 may contribute to increases in AQP-4 and ENaC expression to facilitate water transport across neonatal airway epithelia in the immediate postnatal period.

aquaporin water channel; lung development; neonatal pulmonary function


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
Z. Borok and A. S. Verkman
Lung Edema Clearance: 20 Years of Progress: Invited Review: Role of aquaporin water channels in fluid transport in lung and airways
J Appl Physiol, December 1, 2002; 93(6): 2199 - 2206.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Matalon, A. Lazrak, L. Jain, and D. C. Eaton
Lung Edema Clearance: 20 Years of Progress: Invited Review: Biophysical properties of sodium channels in lung alveolar epithelial cells
J Appl Physiol, November 1, 2002; 93(5): 1852 - 1859.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Horster
Embryonic epithelial membrane transporters
Am J Physiol Renal Physiol, December 1, 2000; 279(6): F982 - F996.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. S. Verkman, M. A. Matthay, and Y. Song
Aquaporin water channels and lung physiology
Am J Physiol Lung Cell Mol Physiol, May 1, 2000; 278(5): L867 - L879.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online