| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pediatrics, University of Kentucky Medical School, Lexington, Kentucky 40536
Elastic fibers are thought to provide structural support for secondary septa as the lung undergoes the transition from the saccular to the alveolar stage. The synthesis of the soluble precursor of elastin, tropoelastin, occurs during a finite developmental period. We have investigated the developmental regulation of tropoelastin gene transcription and mRNA expression in fetal and postnatal rat lung fibroblasts and have assessed the changes in tropoelastin gene expression caused by hyperoxic exposure during secondary septal development. With the use of an RT-PCR assay and intron-specific primers to detect heterogeneous nuclear RNA (hnRNA) and intron-spanning primers to detect mRNA in freshly isolated rat lung fibroblasts, tropoelastin gene expression was found to be upregulated late in gestation. From days 18 to 21 of gestation, there was a 4.5-fold increase in tropoelastin hnRNA (P < 0.0001) and a 6-fold increase in mRNA (P = 0.002). After birth, tropoelastin expression was downregulated. Signals decreased from fetal day 21 to postnatal day 2 for both tropoelastin hnRNA (P = 0.021) and mRNA (P = 0.043). Tropoelastin hnRNA decreased further from days 2 to 6 (P = 0.04). Both tropoelastin hnRNA and mRNA were again upregulated during alveolarization from days 9 to 11, indicating that, once upregulated, transcription of the tropoelastin gene is not constant but varies with fetal and postnatal age. Exposure to >95% oxygen, when initiated on postnatal day 2 or 3 and continued until day 11, significantly diminished the developmental increase in tropoelastin hnRNA (P < 0.005) and mRNA (P < 0.05) normally seen on days 9-11, indicating that the postnatal upregulation of tropoelastin gene expression is inhibited by hyperoxic exposure in the early postnatal period.
developmental regulation of tropoelastin gene transcription; hyperoxia and tropoelastin gene expression; reverse transcription-polymerase chain reaction of intron-specific primers; tropoelastin heterogenous nuclear ribonucleic acid; hyperoxic injury in neonatal lung; bronchopulmonary dysplasia
This article has been cited by other articles:
|
|
 |
|
  L. Cao, J. Wang, I. Tseu, D. Luo, and M. Post Maternal exposure to endotoxin delays alveolarization during postnatal rat lung development Am J Physiol Lung Cell Mol Physiol, May 1, 2009; 296(5): L726 - L737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Boucherat, M.-L. Franco-Montoya, C. Thibault, R. Incitti, B. Chailley-Heu, C. Delacourt, and J. R. Bourbon Gene expression profiling in lung fibroblasts reveals new players in alveolarization Physiol Genomics, December 19, 2007; 32(1): 128 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Mondrinos, S. Koutzaki, P. I. Lelkes, and C. M. Finck A tissue-engineered model of fetal distal lung tissue Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L639 - L650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-P. Kuang, X.-H. Zhang, C. B. Rich, J. A. Foster, M. Subramanian, and R. H. Goldstein Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L944 - L952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. Phelps, D. L. Updike, E. C. Bullen, P. Grammas, and E. W. Howard Transcriptional and posttranscriptional regulation of angiopoietin-2 expression mediated by IGF and PDGF in vascular smooth muscle cells Am J Physiol Cell Physiol, February 1, 2006; 290(2): C352 - C361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-P. Kuang and R. H. Goldstein Regulation of elastin gene transcription by proteasome dysfunction Am J Physiol Cell Physiol, September 1, 2005; 289(3): C766 - C773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-P. Kuang and R. H. Goldstein Regulation of elastin gene transcription by interleukin-1{beta}-induced C/EBP{beta} isoforms Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1349 - C1355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Veness-Meehan, R. A. Pierce, B. M. Moats-Staats, and A. D. Stiles Retinoic acid attenuates O2-induced inhibition of lung septation Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L971 - L980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-P. Kuang, J. L. Berk, D. C. Rishikof, J. A. Foster, D. E. Humphries, D. A. Ricupero, and R. H. Goldstein NF-kappa B induced by IL-1beta inhibits elastin transcription and myofibroblast phenotype Am J Physiol Cell Physiol, July 1, 2002; 283(1): C58 - C65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Srinivasan, J. Strange, F. Awonusonu, and M. C. Bruce Pre- and Postnatal Lung Development, Maturation, and Plasticity: Insulin-like growth factor I receptor is downregulated after alveolarization in an apoptotic fibroblast subset Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L457 - L467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Stenmark Cell-, age-, and phenotype-dependent differences in the control of gene expression Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L762 - L765. [Full Text] [PDF]
|
|
|
|
|
|
A. Liebeskind, S. Srinivasan, D. Kaetzel, and M. Bruce Retinoic acid stimulates immature lung fibroblast growth via a PDGF-mediated autocrine mechanism Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L81 - L90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Cooper, B. Mueck, S. Yousefi, S. Potter, and G. Jarai cDNA-RDA of genes expressed in fetal and adult lungs identifies factors important in development and function Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L284 - L293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Awonusonu, S. Srinivasan, J. Strange, W. Al-Jumaily, and M. C. Bruce Developmental shift in the relative percentages of lung fibroblast subsets: role of apoptosis postseptation Am J Physiol Lung Cell Mol Physiol, October 1, 1999; 277(4): L848 - L859. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
