| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
expression with alveolar epithelial cell
phenotype in vitro
Will Rogers Institute Pulmonary Research Center, Division of Pulmonary and Critical Care Medicine, University of Southern California, Los Angeles, California 90033; and The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118
T1
is a
recently identified gene expressed in the adult rat lung by alveolar
type I (AT1) epithelial cells but not by alveolar type II (AT2)
epithelial cells. We evaluated the effects of modulating alveolar
epithelial cell (AEC) phenotype in vitro on T1 expression using
either soluble factors or changes in cell shape to influence phenotype.
For studies on the effects of soluble factors on T1 expression, rat
AT2 cells were grown on polycarbonate filters in serum-free medium
(MDSF) or in MDSF supplemented with either bovine serum (BS, 10%), rat
serum (RS, 5%), or keratinocyte growth factor (KGF, 10 ng/ml) from
either day 0 or day
4 through day 8 in
culture. For studies on the effects of cell shape on T1 expression,
AT2 cells were plated on thick collagen gels in MDSF supplemented with
BS. Gels were detached on either day 1 (DG1) or day 4 (DG4) or were left
attached until day 8. RNA and protein were harvested at intervals between days
1 and 8 in culture,
and T1 expression was quantified by Northern and Western blotting, respectively. Expression of T1 progressively increases in AEC grown
in MDSF ± BS between day 1 and
day 8 in culture, consistent with
transition toward an AT1 cell phenotype. Exposure to RS or KGF from
day 0 prevents the increase in T1
expression on day 8, whereas addition
of either factor from day 4 through
day 8 reverses the increase. AEC
cultured on attached gels express high levels of T1 on
days 4 and
8. T1 expression is markedly
inhibited in both DG1 and DG4 cultures, consistent with both inhibition and reversal of the transition toward the AT1 cell phenotype. These
results demonstrate that both soluble factors and alterations in cell
shape modulate T1 expression in parallel with AEC phenotype and
provide further support for the concept that transdifferentiation between AT2 and AT1 cell phenotypes is at least partially reversible.
collagen gel; keratinocyte growth factor; rat serum; transdifferentiation; type I cell
This article has been cited by other articles:
|
|
 |
|
  L. DeMaio, W. Tseng, Z. Balverde, J. R. Alvarez, K.-J. Kim, D. G. Kelley, R. M. Senior, E. D. Crandall, and Z. Borok Characterization of mouse alveolar epithelial cell monolayers Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L1051 - L1058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Clegg, C. Tyrrell, S. R. McKechnie, M. F. Beers, D. Harrison, and M. C. McElroy Coexpression of RTI40 with alveolar epithelial type II cell proteins in lungs following injury: identification of alveolar intermediate cell types Am J Physiol Lung Cell Mol Physiol, September 1, 2005; 289(3): L382 - L390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Patel, D. Reigada, C. H. Mitchell, S. R. Bates, S. S. Margulies, and M. Koval Paracrine stimulation of surfactant secretion by extracellular ATP in response to mechanical deformation Am J Physiol Lung Cell Mol Physiol, September 1, 2005; 289(3): L489 - L496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Brock, Y.-J. Lee, E. Maydanski, T. L. Marburger, M. Luo, R. Paine III, and M. Peters-Golden Nuclear localization of leukotriene A4 hydrolase in type II alveolar epithelial cells in normal and fibrotic lung Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L224 - L232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gonzalez, Y. H. Yang, C. Griffin, L. Allen, Z. Tigue, and L. Dobbs Freshly isolated rat alveolar type I cells, type II cells, and cultured type II cells have distinct molecular phenotypes Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L179 - L189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Narasaraju, N. Jin, C. R. Narendranath, Z. Chen, D. Gou, and L. Liu Protein nitration in rat lungs during hyperoxia exposure: a possible role of myeloperoxidase Am J Physiol Lung Cell Mol Physiol, November 1, 2003; 285(5): L1037 - L1045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Roper, R. J. Staversky, J. N. Finkelstein, P. C. Keng, and M. A. O'Reilly Identification and isolation of mouse type II cells on the basis of intrinsic expression of enhanced green fluorescent protein Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L691 - L700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. Crandall and R. M. Effros Lung Edema Clearance: 20 Years of Progress: Historical perspectives on lung edema clearance J Appl Physiol, October 1, 2002; 93(4): 1527 - 1532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Ramirez, L. Pollack, G. Millien, Y. X. Cao, A. Hinds, and M. C. Williams The {alpha}-Isoform of Caveolin-1 Is a Marker of Vasculogenesis in Early Lung Development J. Histochem. Cytochem., January 1, 2002; 50(1): 33 - 42. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Guo, C. Martinez-Williams, C. E. Yellowley, H. J. Donahue, and D. E. Rannels Connexin expression by alveolar epithelial cells is regulated by extracellular matrix Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L191 - L202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yano, R. J. Mason, T. Pan, R. R. Deterding, L. D. Nielsen, and J. M. Shannon KGF regulates pulmonary epithelial proliferation and surfactant protein gene expression in adult rat lung Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1146 - L1158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Y. Li, K. Hirayoshi, and Y. Suzuki Expression of N-deacetylase/sulfotransferase and 3-O-sulfotransferase in rat alveolar type II cells Am J Physiol Lung Cell Mol Physiol, August 1, 2000; 279(2): L292 - L301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Lubman, X.-L. Zhang, J. Zheng, L. Ocampo, M. Z. Lopez, S. Veeraraghavan, S. M. Zabski, S. I. Danto, and Z. Borok Integrin alpha 3-subunit expression modulates alveolar epithelial cell monolayer formation Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L183 - L193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. McElroy, H. R. Harty, G. E. Hosford, G. M. Boylan, J.-F. Pittet, and T. J. Foster Alpha-Toxin Damages the Air-Blood Barrier of the Lung in a Rat Model of Staphylococcus aureus-Induced Pneumonia Infect. Immun., October 1, 1999; 67(10): 5541 - 5544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Borok, X. Li, V. F. J. Fernandes, B. Zhou, D. K. Ann, and E. D. Crandall Differential Regulation of Rat Aquaporin-5 Promoter/Enhancer Activities in Lung and Salivary Epithelial Cells J. Biol. Chem., August 18, 2000; 275(34): 26507 - 26514. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
