AJP - Lung Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 275: L165-L171, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Narasimhan, S. R.
Right arrow Articles by Broaddus, V. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Narasimhan, S. R.
Right arrow Articles by Broaddus, V. C.
Vol. 275, Issue 1, L165-L171, July 1998

Resistance of pleural mesothelioma cell lines to apoptosis: relation to expression of Bcl-2 and Bax

Sudha Rani Narasimhan1, Lin Yang1, Brenda I. Gerwin2, and V. Courtney Broaddus1

1 Department of Medicine and Lung Biology Center, San Francisco General Hospital, San Francisco, California 94110; and 2 Division of Basic Sciences, National Cancer Institute, Bethesda, Maryland 20892

A failure of normal apoptosis, often due to mutant p53, may contribute to the formation of a cancer and to its resistance to therapy. Mesothelioma, an asbestos-induced tumor, is highly resistant to therapy but generally expresses wild-type p53. We asked whether mesothelioma was resistant to apoptosis and whether resistance was associated with altered expression of the antiapoptotic protein Bcl-2 or proapoptotic protein Bax. We found that three mesothelioma cell lines (1 with wild-type p53) were highly resistant to apoptosis induced by oxidant stimuli (asbestos, H2O2) or nonoxidant stimuli (calcium ionophore) compared with primary cultured mesothelial cells. By immunostaining, one of these three lines expressed Bcl-2 but only during mitosis. By immunoblotting, 3 of 14 additional mesothelioma lines (9 of 14 with wild type p53) expressed Bcl-2 but all 14 of 14 expressed the proapoptotic Bax, giving a low ratio of Bcl-2 to Bax. We conclude that mesothelioma cell lines are resistant to apoptosis and that the failure in apoptosis is not explained by Bcl-2 but by other mechanisms that counteract the proapoptotic effect of Bax.

mesothelial cell; p53; reactive oxygen species; asbestos; cancer


This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
L. Nici, B. Monfils, and P. Calabresi
The Effects of Taurolidine, a Novel Antineoplastic Agent, on Human Malignant Mesothelioma
Clin. Cancer Res., November 15, 2004; 10(22): 7655 - 7661.
[Abstract] [Full Text] [PDF]

Home page
 Exp Biol MedHome page
D. Upadhyay and D. W. Kamp
Asbestos-Induced Pulmonary Toxicity: Role of DNA Damage and Apoptosis
Exp Biol Med, June 1, 2003; 228(6): 650 - 659.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
C. Xia, Z. Xu, X. Yuan, K. Uematsu, L. You, K. Li, L. Li, F. McCormick, and D. M. Jablons
Induction of Apoptosis in Mesothelioma Cells by Antisurvivin Oligonucleotides
Mol. Cancer Ther., July 1, 2002; 1(9): 687 - 694.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
W. R. Smythe, I. Mohuiddin, M. Ozveran, and X. X. Cao
Antisense therapy for malignant mesothelioma with oligonucleotides targeting the bcl-xl gene product
J. Thorac. Cardiovasc. Surg., June 1, 2002; 123(6): 1191 - 1198.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
X. X. Cao, I. Mohuiddin, F. Ece, D. J. McConkey, and W. R. Smythe
Histone Deacetylase Inhibitor Downregulation of bcl-xl Gene Expression Leads to Apoptotic Cell Death in Mesothelioma
Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 562 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. J. Metheny-Barlow, B. Flynn, H. E. van Gijssel, A. Marrogi, and B. I. Gerwin
Paradoxical Effects of Platelet-Derived Growth Factor-A Overexpression in Malignant Mesothelioma . Antiproliferative Effects In Vitro and Tumorigenic Stimulation In Vivo
Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 694 - 702.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Marrogi, H. I. Pass, M. Khan, L. J. Metheny-Barlow, C. C. Harris, and B. I. Gerwin
Human Mesothelioma Samples Overexpress Both Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (NOS2): In Vitro Antiproliferative Effects of a COX-2 Inhibitor
Cancer Res., July 1, 2000; 60(14): 3696 - 3700.
[Abstract] [Full Text]

Home page
 Clin. Cancer Res.Home page
Y. Soini, V. Kinnula, R. Kaarteenaho-Wiik, E. Kurttila, K. Linnainmaa, and P. Paakko
Apoptosis and Expression of Apoptosis Regulating Proteins bcl-2, mcl-1, bcl-X, and bax in Malignant Mesothelioma
Clin. Cancer Res., November 1, 1999; 5(11): 3508 - 3515.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
D. W Kamp and S. A Weitzman
The molecular basis of asbestos induced lung injury
Thorax, July 1, 1999; 54(7): 638 - 652.
[Full Text]

Home page
 CarcinogenesisHome page
L. Xu, B. J. Flynn, S. Ungar, H. I. Pass, K. Linnainmaa, K. Mattson, and B. I. Gerwin
Asbestos induction of extended lifespan in normal human mesothelial cells: interindividual susceptibility and SV40 T antigen
Carcinogenesis, May 1, 1999; 20(5): 773 - 783.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online