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Am J Physiol Lung Cell Mol Physiol 275: L193-L199, 1998;
1040-0605/98 $5.00
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Vol. 275, Issue 1, L193-L199, July 1998

SPECIAL COMMUNICATION
Characterization of a mitogen-activated protein kinase from Pneumocystis carinii

Charles F. Thomas Jr.1, Theodore J. Kottom1, Edward B. Leof1,2, and Andrew H. Limper1,2

1 Thoracic Diseases Research Unit, Division of Pulmonary, Critical Care and Internal Medicine and 2 Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, Minnesota 55905

The pathogenic fungus Pneumocystis carinii causes severe pneumonia in patients with impaired immunity, particularly patients with acquired immunodeficiency syndrome. The life cycle of P. carinii is poorly understood, and the inability to continuously culture P. carinii is a major limitation in understanding its cell biology. In fungi homologous to P. carinii, pheromone mating factors signal through a mitogen-activated protein kinase (MAPK) signal transduction cascade, resulting in mitotic cell cycle arrest and entry into a pathway of conjugation, cellular differentiation, and proliferation. Using degenerate PCR and library screening, we have identified a MAPK cDNA in P. carinii that is highly homologous to fungal MAPKs involved in the pheromone mating signal transduction cascade, and we demonstrate MAPK activity in P. carinii lysates with a specific antiserum derived from the translated P. carinii MAPK cDNA sequence.

acquired immunodeficiency syndrome; serine-threonine protein kinase; signal transduction; molecular cloning


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