| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
and
PMA in human monocytic U-937 cells
1 Combined Program in Pulmonary
and Critical Care Medicine,
Regulation of eotaxin expression was
investigated in U-937 cells, a human monocyte-like cell line. Eotaxin
mRNA was induced by tumor necrosis factor-
(TNF-; 0.1-100
ng/ml) and phorbol 12-myristate 13-acetate (PMA; 0.01-1 µM).
PMA-induced eotaxin mRNA expression was of greater magnitude and was
maximal at a later time point than TNF--induced expression (16 h vs.
2 h after stimulation), which was consistent with eotaxin protein
expression detected by immunocytochemistry. Dexamethasone (0.01-10
µM) decreased eotaxin mRNA expression in both TNF-- and
PMA-stimulated U-937 cells. PMA-induced eotaxin mRNA expression was
inhibited by cycloheximide (10 µg/ml), whereas TNF--induced
expression was not. The protein kinase C (PKC) inhibitor staurosporine
(10-50 nM) inhibited PMA-induced eotaxin mRNA expression, whereas
TNF--induced expression was enhanced by this reagent. These results
suggest that eotaxin expression can be induced by more than one
mechanism: the PMA-triggered pathway is mediated by PKC activation and
requires new protein synthesis, whereas the TNF--triggered pathway
is independent of PKC and protein synthesis. TNF-- and PMA-induced
pathways are both associated with nuclear factor-
B, because its
binding activity was enhanced in the presence of these stimuli, and
both pathways were limited by its inhibitor, diethyldithiocarbamate.
dexamethasone; protein kinase C; nuclear factor-B; cycloheximide
This article has been cited by other articles:
|
|
 |
|
  H. Nakamura, A. D. Luster, H. Tateno, S. Jedrzkiewicz, G. Tamura, K. J. Haley, E. A. Garcia-Zepeda, K. Yamaguchi, and C. M. Lilly IL-4 differentially regulates eotaxin and MCP-4 in lung epithelium and circulating mononuclear cells Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1288 - L1302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. LILLY, H. NAKAMURA, O. I. BELOSTOTSKY, K. J. HALEY, E. A. GARCIA-ZEPEDA, A. D. LUSTER, and E. ISRAEL Eotaxin Expression after Segmental Allergen Challenge in Subjects with Atopic Asthma Am. J. Respir. Crit. Care Med., June 1, 2001; 163(7): 1669 - 1675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-F. Guo, A. B. Lentsch, R. L. Warner, M. Huber-Lang, J. V. Sarma, T. Hlaing, M. M. Shi, N. W. Lukacs, and P. A. Ward Regulatory Effects of Eotaxin on Acute Lung Inflammatory Injury J. Immunol., April 15, 2001; 166(8): 5208 - 5218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jedrzkiewicz, H. Nakamura, E. S. Silverman, A. D. Luster, N. Mansharamani, K. H. In, G. Tamura, and C. M. Lilly IL-1beta induces eotaxin gene transcription in A549 airway epithelial cells through NF-kappa B Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1058 - L1065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Rothenberg Eotaxin . An Essential Mediator of Eosinophil Trafficking into Mucosal Tissues Am. J. Respir. Cell Mol. Biol., September 1, 1999; 21(3): 291 - 295. [Full Text]
|
|
|
|
 |
|
 P. R. Mittelstadt and J. D. Ashwell Inhibition of AP-1 by the Glucocorticoid-inducible Protein GILZ J. Biol. Chem., July 27, 2001; 276(31): 29603 - 29610. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
