AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 275: L861-L869, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, R.-M.
Right arrow Articles by Forman, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, R.-M.
Right arrow Articles by Forman, H. J.
Vol. 275, Issue 5, L861-L869, November 1998

gamma -Glutamylcysteine synthetase: mRNA stabilization and independent subunit transcription by 4-hydroxy-2-nonenal

Rui-Ming Liu, Lin Gao, Jinah Choi, and Henry Jay Forman

Department of Molecular Pharmacology and Toxicology, University of Southern California, Los Angeles, California 90033

gamma -Glutamylcysteine synthetase (GCS), the rate-limiting enzyme in de novo glutathione (GSH) synthesis, is composed of one catalytic (heavy) and one regulatory (light) subunit. Although both subunits are increased at the mRNA level by oxidants, it is not clear whether they are regulated through the same mechanism. 4-Hydroxy-2-nonenal (4HNE), a lipid peroxidation product, may act as a mediator for the induction of gene expression by oxidants. In the present study, 4HNE was used to study the mechanism of induction of the two GCS subunits in rat lung epithelial L2 cells. 4HNE increased both the transcription rates and the stability of mRNA for both GCS subunits, resulting in an increased mRNA content for both subunits. Both GCS subunit proteins and enzymatic activities also increased. Emetine, a protein synthesis inhibitor, blocked the increase in GCS light subunit mRNA but not the increase in GCS heavy subunit mRNA. This suggested that although 4HNE increased transcription and stabilization of both GCS subunit mRNAs, the signaling pathways involved in the induction of the two GCS subunits differed.

messenger ribonucleic acid; oxidative stress; regulatory subunit; catalytic subunit; protein synthesis inhibitor; glutathione


This article has been cited by other articles:


Home page
 Eur Respir JHome page
I. Rahman and I. M. Adcock
Oxidative stress and redox regulation of lung inflammation in COPD.
Eur. Respir. J., July 1, 2006; 28(1): 219 - 242.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Numazawa, M. Ishikawa, A. Yoshida, S. Tanaka, and T. Yoshida
Atypical protein kinase C mediates activation of NF-E2-related factor 2 in response to oxidative stress
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C334 - C342.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Watanabe, D. A. Dickinson, D. M. Krzywanski, K. E. Iles, H. Zhang, C. J. Venglarik, and H. J. Forman
A549 subclones demonstrate heterogeneity in toxicological sensitivity and antioxidant profile
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L726 - L736.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R.-M. Liu, Z. Borok, and H. J. Forman
4-Hydroxy-2-nonenal Increases {gamma}-Glutamylcysteine Synthetase Gene Expression in Alveolar Epithelial Cells
Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 499 - 505.
[Abstract] [Full Text]

Home page
 IOVSHome page
R. Kannan, B. Ouyang, E. Wawrousek, N. Kaplowitz, and U. P. Andley
Regulation of GSH in {{alpha}}A-Expressing Human Lens Epithelial Cell Lines and in {{alpha}}A Knockout Mouse Lenses
Invest. Ophthalmol. Vis. Sci., February 1, 2001; 42(2): 409 - 416.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. A. A. West, A. R. Buckpitt, and C. G. Plopper
Elevated Airway GSH Resynthesis Confers Protection to Clara Cells from Naphthalene Injury in Mice Made Tolerant by Repeated Exposures
J. Pharmacol. Exp. Ther., August 1, 2000; 294(2): 516 - 523.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. Choi, R.-M. Liu, R. K. Kundu, F. Sangiorgi, W. Wu, R. Maxson, and H. J. Forman
Molecular Mechanism of Decreased Glutathione Content in Human Immunodeficiency Virus Type 1 Tat-transgenic Mice
J. Biol. Chem., February 4, 2000; 275(5): 3693 - 3698.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Rahman and W. MacNee
Lung glutathione and oxidative stress: implications in cigarette smoke-induced airway disease
Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1067 - L1088.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. C. LU
Regulation of hepatic glutathione synthesis: current concepts and controversies
FASEB J, July 1, 1999; 13(10): 1169 - 1183.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. Jha, O. Jurma, G. Lalli, Y. Liu, E. H. Pettus, J. T. Greenamyre, R.-M. Liu, H. J. Forman, and J. K. Andersen
Glutathione Depletion in PC12 Results in Selective Inhibition of Mitochondrial Complex I Activity. IMPLICATIONS FOR PARKINSON'S DISEASE
J. Biol. Chem., August 18, 2000; 275(34): 26096 - 26101.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Mari and A. I. Cederbaum
CYP2E1 Overexpression in HepG2 Cells Induces Glutathione Synthesis by Transcriptional Activation of gamma -Glutamylcysteine Synthetase
J. Biol. Chem., May 12, 2000; 275(20): 15563 - 15571.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online