Vol. 275, Issue 5, L983-L989, November 1998
Shedding of L-selectin and PECAM-1 and upregulation of Mac-1
and ICAM-1 on neutrophils in RSV bronchiolitis
Shan-Ze
Wang1,2,
Peter K.
Smith1,
Melanie
Lovejoy1,
Jeffrey J.
Bowden2,
John H.
Alpers2, and
Kevin D.
Forsyth1
Departments of 1 Paediatrics
and 2 Respiratory Medicine,
Flinders Medical Centre, Flinders University, Bedford Park, South
Australia 5042, Australia
Bronchiolitis is
characterized histologically by epithelial necrosis and peribronchial
infiltration of leukocytes, with a high percentage of neutrophils in
the airways. We investigated the expression of adhesion molecules
(CD11a, CD11b, CD18, CD31, CD54, and CD62L) on neutrophils from
nasopharyngeal aspirates (NPAs) and peripheral blood (PB) of infants
with respiratory syncytial virus (RSV)-induced bronchiolitis. The
expression of CD31 and CD62L on neutrophils from NPAs is decreased and
the expression of CD11b, CD18, and CD54 on neutrophils from NPAs is
increased compared with cells from PB of RSV-infected infants. The
expression of CD18 and CD54 on neutrophils from PB of RSV-infected
infants is also increased compared with cells from PB of control
infants. Shedding of CD31 and CD62L on neutrophils in RSV infection may contribute to the neutrophil emigration from blood to airways; the
upregulation of Mac-1 (CD11b/CD18) and CD54 on neutrophils may help
explain the high percentage of neutrophils in the airways of RSV
bronchiolitis; and the upregulation of Mac-1 may be involved in the
increased neutrophil-airway epithelial adhesion in RSV infection.
platelet endothelial cell adhesion molecule-1; intercellular
adhesion molecule-1; respiratory syncytial virus; polymorphonuclear
leukocyte; adhesion molecule expression; emigration; airway; viral
infection
