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Am J Physiol Lung Cell Mol Physiol 275: L1051-L1060, 1998;
1040-0605/98 $5.00
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Vol. 275, Issue 6, L1051-L1060, December 1998

Effects of hypoxia on energy state and pH in resting pulmonary and femoral arterial smooth muscles

R. M. Leach1, D. W. Sheehan1, V. P. Chacko2, and J. T. Sylvester1

1 Division of Pulmonary and Critical Care Medicine, Department of Medicine, and 2 Division of Nuclear Magnetic Resonance, Department of Radiology and Radiological Science, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21224

To determine the effects of hypoxia on energy state and intracellular pH (pHi) in resting pulmonary and systemic arterial smooth muscles, we used 31P nuclear magnetic resonance spectroscopy and colorimetric and enzymatic assays to measure pHi; intracellular concentrations of ATP, phosphocreatine, creatine, and Pi; and phosphorylation potential in superfused tissue segments from porcine proximal intrapulmonary and superficial femoral arteries. Under baseline conditions (PO2 467 ± 12.1 mmHg), energy state and total creatine (phosphocreatine + creatine) concentration were lower and pHi was higher in pulmonary arteries. During hypoxia (PO2 23 ± 2.4 mmHg), energy state deteriorated more in femoral arteries than in pulmonary arteries. pHi fell in both tissues but was always more alkaline in pulmonary arteries. Reoxygenation reversed the changes induced by hypoxia. These results suggest that production and/or elimination of ATP and H+ was different in resting pulmonary and systemic arterial smooth muscles under baseline and hypoxic conditions. Because energy state and pHi affect a wide variety of cellular processes, including signal transduction, contractile protein interaction, and activities of ion pumps and channels, further investigation is indicated to determine whether these differences have functional significance.

phosphorus-31 nuclear magnetic resonance; phosphorylation potential; adenosine 5'-triphosphate; phosphocreatine; creatine; vascular smooth muscle; intracellular water volume


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