AJP - Lung Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 275: L1110-L1119, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barrett, E. G.
Right arrow Articles by Finkelstein, J. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barrett, E. G.
Right arrow Articles by Finkelstein, J. N.
Vol. 275, Issue 6, L1110-L1119, December 1998

Silica-induced chemokine expression in alveolar type II cells is mediated by TNF-alpha

Edward G. Barrett1, Carl Johnston1,2, Günter Oberdörster1, and Jacob N. Finkelstein1,2

Departments of 1 Environmental Medicine and 2 Pediatrics, University of Rochester School of Medicine, Rochester, New York 14642

Recent evidence has suggested that epithelial cells may contribute to the inflammatory response in the lung after exposure to crystalline silica through the production of and response to specific growth factors, chemokines, and cytokines. However, the exact cellular and molecular responses of epithelial cells to silica exposure remains unclear. Using a murine alveolar type II cell line [murine lung epithelial (MLE)-15 cell line], we measured the early changes in various cytokine and chemokine mRNA species after exposure of the cells to 4-35 µg/cm2 of silica (cristobalite), interferon (IFN)-gamma , tumor necrosis factor (TNF)-alpha , and lipopolysaccharide (LPS) alone or in combination. Total mRNA was isolated and assayed with an RNase protection assay after 6 and 24 h of exposure. Cristobalite exposure alone led to an increase in monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-2, and regulated on activation normal T cells expressed and secreted (RANTES) mRNAs. Treatment with IFN-gamma alone increased MCP-1 mRNA levels. Treatment with TNF-alpha or LPS alone led to an increase in MCP-1 and MIP-2 mRNA. The combination of cristobalite plus TNF-alpha led to an additive increase in MCP-1 and MIP-2, whereas cristobalite plus IFN-gamma or LPS had a synergistic effect. We also found with a TNF-alpha -neutralizing antibody that TNF-alpha plays a major role in mediating the type II cell chemokine response to cristobalite exposure. The results indicate that the cristobalite-induced chemokine response in the lung epithelium is mediated in part by TNF-alpha and can be enhanced by macrophage- and lymphocyte-derived inflammatory mediators in an additive and synergistic fashion.

airway epithelium; interferon-gamma ; lipopolysaccharide; monocyte chemotactic protein-1; macrophage inflammatory protein-2; regulated on activation normal T cells expressed and secreted; tumor necrosis factor-alpha


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. R. Wilson, S. Choudhury, and M. Takata
Pulmonary inflammation induced by high-stretch ventilation is mediated by tumor necrosis factor signaling in mice
Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L599 - L607.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. A. Johnston, J. P. Mizgerd, and S. A. Shore
CXCR2 is essential for maximal neutrophil recruitment and methacholine responsiveness after ozone exposure
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L61 - L67.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. R. Wilson, S. Choudhury, M. E. Goddard, K. P. O'Dea, A. G. Nicholson, and M. Takata
High tidal volume upregulates intrapulmonary cytokines in an in vivo mouse model of ventilator-induced lung injury
J Appl Physiol, October 1, 2003; 95(4): 1385 - 1393.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. A. Jimenez, E. M. Drost, P. S. Gilmour, I. Rahman, F. Antonicelli, H. Ritchie, W. MacNee, and K. Donaldson
PM10-exposed macrophages stimulate a proinflammatory response in lung epithelial cells via TNF-alpha
Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L237 - L248.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. G. Barrett, C. Johnston, G. Oberdorster, and J. N. Finkelstein
Silica-induced chemokine expression in alveolar type II cells is mediated by TNF-alpha -induced oxidant stress
Am J Physiol Lung Cell Mol Physiol, June 1, 1999; 276(6): L979 - L988.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online