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Am J Physiol Lung Cell Mol Physiol 275: L1208-L1218, 1998;
1040-0605/98 $5.00
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Vol. 275, Issue 6, L1208-L1218, December 1998

Alveolar macrophage apoptosis and TNF-alpha , but not p53, expression correlate with murine response to bleomycin

Luis A. Ortiz1, Kryztof Moroz2, Jing-Yao Liu2, Gary W. Hoyle1, Timothy Hammond3, Raymond F. Hamilton4, Andrij Holian4, William Banks5, Arnold R. Brody2, and Mitchell Friedman1

Sections of 1 Pulmonary Diseases, Critical Care and Environmental Medicine and 3 Nephrology, Department of Medicine, and 2 Lung Biology Program, Department of Pathology, Tulane University Medical Center, New Orleans, Louisiana 70112; 4 Division of Pulmonary and Critical Care Medicine, Department of Medicine, The University of Texas Medical School, Houston, Texas 77030; and 5 Division of Geriatrics, St. Louis University, St. Louis, Missouri 63104

Apoptosis is considered to be a protective mechanism that limits lung injury. However, apoptosis might contribute to the inflammatory burden present in the injured lung. The exposure of mice to bleomycin (BLM) is a well-established model for the study of lung injury. BLM exposure induces DNA damage and enhances tumor necrosis factor (TNF)-alpha expression in the lung. To evaluate the importance of alveolar macrophage (AM) apoptosis in the pathogenesis of lung injury, we exposed BLM-sensitive (C57BL/6) and BLM-resistant (BALB/c) mice to BLM (120 mg/kg) and studied the induction of apoptosis [by light-microscopy changes (2, 8, 12, 24, 48, and 72 h) and annexin V uptake by flow cytometry (24 h)], the secretion of TNF-alpha (measured by ELISA), and the expression of p53 (by immunoblotting) in AM retrieved from these mice. BLM, but not vehicle, induced apoptosis in AM from both murine strains. The numbers of apoptotic AM were significantly greater (P < 0.001) in C57BL/6 mice (52.9%) compared with BALB/c mice (40.8%) as demonstrated by annexin V uptake. BLM induction of apoptosis in AM was preceded by an increased secretion of TNF-alpha in C57BL/6 but not in BALB/c mice. Furthermore, double TNF-alpha receptor-deficient mice, developed on a C57BL/6 background, demonstrated significantly (P < 0.001) lower numbers of apoptotic AM compared with C57BL/6 and BALB/c mice. BLM also enhanced p53 expression in AM from both murine strains. However, p53-deficient mice developed BLM-induced lung injury, exhibited similar lung cell proliferation (measured as proliferating cell nuclear antigen immunostaining), and accumulated similar amounts of lung hydroxyproline (65 ± 6.9 µg/lung) as did C57BL/6 (62 ± 6.5 µg/lung) mice. Therefore, AM apoptosis is occurring during BLM-induced lung injury in a manner that correlates with murine strain sensitivity to BLM. Furthermore, TNF-alpha secretion rather than p53 expression contributes to the difference in murine strain response to BLM.

tumor necrosis factor; strain susceptibility


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