AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 276: L20-L27, 1999;
1040-0605/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertorello, A. M.
Right arrow Articles by Sznajder, J. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertorello, A. M.
Right arrow Articles by Sznajder, J. I.
Vol. 276, Issue 1, L20-L27, January 1999

Isoproterenol increases Na+-K+-ATPase activity by membrane insertion of alpha -subunits in lung alveolar cells

Alejandro M. Bertorello1, Karen M. Ridge2, Alexander V. Chibalin1, Adrian I. Katz3, and Jacob I. Sznajder2

1 Department of Molecular Medicine, Karolinska Institutet, Karolinska Hospital, S-171 76 Stockholm, Sweden; 2 Department of Pulmonary and Critical Care Medicine, Michael Reese Hospital, University of Illinois at Chicago, Chicago 60616; and 3 Department of Medicine, University of Chicago, Chicago, Illinois 60637

Catecholamines promote lung edema clearance via beta -adrenergic-mediated stimulation of active Na+ transport across the alveolar epithelium. Because alveolar epithelial type II cell Na+-K+-ATPase contributes to vectorial Na+ flux, the present study was designed to investigate whether Na+-K+-ATPase undergoes acute changes in its catalytic activity in response to beta -adrenergic-receptor stimulation. Na+-K+-ATPase activity increased threefold in cells incubated with 1 µM isoproterenol for 15 min, which also resulted in a fourfold increase in the cellular levels of cAMP. Forskolin (10 µM) also stimulated Na+-K+-ATPase activity as well as ouabain binding. The increase in Na+-K+-ATPase activity was abolished when cells were coincubated with a cAMP-dependent protein kinase inhibitor. This stimulation, however, was not due to protein kinase-dependent phosphorylation of the Na+-K+-ATPase alpha -subunit; rather, it was the result of an increased number of alpha -subunits recruited from the late endosomes into the plasma membrane. The recruitment of alpha -subunits to the plasma membrane was prevented by stabilizing the cortical actin cytoskeleton with phallacidin or by blocking anterograde transport with brefeldin A but was unaffected by coincubation with amiloride. In conclusion, isoproterenol increases Na+-K+-ATPase activity in alveolar type II epithelial cells by recruiting alpha -subunits into the plasma membrane from an intracellular compartment in an Na+-independent manner.

alveolar epithelium; protein kinases; actin cytoskeleton; sodium transport; early endosomes; late endosomes


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Adir, L. C. Welch, V. Dumasius, P. Factor, J. I. Sznajder, and K. M. Ridge
Overexpression of the Na-K-ATPase {alpha}2-subunit improves lung liquid clearance during ventilation-induced lung injury
Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1233 - L1237.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Frank, R. Briot, J. W. Lee, A. Ishizaka, T. Uchida, and M. A. Matthay
Physiological and biochemical markers of alveolar epithelial barrier dysfunction in perfused human lungs
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L52 - L59.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. G. Folkesson and M. A. Matthay
Alveolar Epithelial Ion and Fluid Transport: Recent Progress
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 10 - 19.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. M. Mutlu and J. I. Sznajder
Mechanisms of pulmonary edema clearance
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L685 - L695.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Mordasini, M. Bustamante, M. Rousselot, P.-Y. Martin, U. Hasler, and E. Feraille
Stimulation of Na+ transport by AVP is independent of PKA phosphorylation of the Na-K-ATPase in collecting duct principal cells
Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1031 - F1039.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. R. Looney, C. Sartori, S. Chakraborty, P. F. James, J. B. Lingrel, and M. A. Matthay
Decreased expression of both the {alpha}1- and {alpha}2-subunits of the Na-K-ATPase reduces maximal alveolar epithelial fluid clearance
Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L104 - L110.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. P. Thomas, J. R. Campbell, P. J. Wright, and R. F. Husted
cAMP-stimulated Na+ transport in H441 distal lung epithelial cells: role of PKA, phosphatidylinositol 3-kinase, and sgk1
Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L843 - L851.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Leroy, A. Dagenais, Y. Berthiaume, and E. Brochiero
Molecular identity and function in transepithelial transport of KATP channels in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L1027 - L1037.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Berthiaume
Long-term stimulation of alveolar epithelial cells by {beta}-adrenergic agonists: increased Na+ transport and modulation of cell growth?
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L798 - L801.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Pesce, A. Comellas, and J. I. Sznajder
{beta}-Adrenergic agonists regulate Na-K-ATPase via p70S6k
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L802 - L807.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Lei, S. Nowbar, C. N. Mariash, and D. H. Ingbar
Thyroid hormone stimulates Na-K-ATPase activity and its plasma membrane insertion in rat alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L762 - L772.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. E. Yang, H. Zhong, P. K. K. Leong, A. Perianayagam, V. M. Campese, and A. A. McDonough
Chronic renal injury-induced hypertension alters renal NHE3 distribution and abundance
Am J Physiol Renal Physiol, May 1, 2003; 284(5): F1056 - F1065.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. E. Yang, P. K. K. Leong, S. Ye, V. M. Campese, and A. A. McDonough
Responses of proximal tubule sodium transporters to acute injury-induced hypertension
Am J Physiol Renal Physiol, February 1, 2003; 284(2): F313 - F322.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y. Berthiaume, H. G. Folkesson, and M. A. Matthay
Lung Edema Clearance: 20 Years of Progress: Invited Review: Alveolar edema fluid clearance in the injured lung
J Appl Physiol, December 1, 2002; 93(6): 2207 - 2213.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
V. A. Narkar, T. Hussain, and M. F. Lokhandwala
Activation of D2-like receptors causes recruitment of tyrosine-phosphorylated NKA alpha 1-subunits in kidney
Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1290 - F1295.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. A. Matthay, C. Clerici, and G. Saumon
Lung Edema Clearance: 20 Years of Progress: Invited Review: Active fluid clearance from the distal air spaces of the lung
J Appl Physiol, October 1, 2002; 93(4): 1533 - 1541.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Fisher and S. S. Margulies
Na+-K+-ATPase activity in alveolar epithelial cells increases with cyclic stretch
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L737 - L746.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. A. Matthay, H. G. Folkesson, and C. Clerici
Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema
Physiol Rev, July 1, 2002; 82(3): 569 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
F. J. Saldias, A. P. Comellas, L. Pesce, E. Lecuona, and J. I. Sznajder
Dopamine increases lung liquid clearance during mechanical ventilation
Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L136 - L143.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. M. Hardiman and S. Matalon
Modification of Sodium Transport and Alveolar Fluid Clearance by Hypoxia . Mechanisms and Physiological Implications
Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 538 - 541.
[Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. L. Vivona, M. Matthay, M. B. Chabaud, G. Friedlander, and C. Clerici
Hypoxia Reduces Alveolar Epithelial Sodium and Fluid Transport in Rats . Reversal by beta -Adrenergic Agonist Treatment
Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 554 - 561.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. Songu-Mize, N. Sevieux, X. Liu, and M. Jacobs
Effect of short-term cyclic stretch on sodium pump activity in aortic smooth muscle cells
Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H2072 - H2078.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. W. McGraw, N. Fukuda, P. F. James, S. L. Forbes, A. L. Woo, J. B. Lingrel, D. P. Witte, M. A. Matthay, and S. B. Liggett
Targeted transgenic expression of {beta}2-adrenergic receptors to type II cells increases alveolar fluid clearance
Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L895 - L903.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Guerrero, E. Lecuona, L. Pesce, K. M. Ridge, and J. I. Sznajder
Dopamine regulates Na-K-ATPase in alveolar epithelial cells via MAPK-ERK-dependent mechanisms
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L79 - L85.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, C. Denis, M.-F. Vives, S. Girouard, C. Masse, T. Nguyen, T. Yamagata, C. Grygorczyk, R. Kothary, and Y. Berthiaume
Modulation of {alpha}-ENaC and {alpha}1-Na+-K+-ATPase by cAMP and dexamethasone in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L217 - L230.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. J. Sweadner and M. S. Feschenko
Predicted location and limited accessibility of protein kinase A phosphorylation site on Na-K-ATPase
Am J Physiol Cell Physiol, April 1, 2001; 280(4): C1017 - C1026.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Aydemir-Koksoy and J. C. Allen
Regulation of Na+ pump expression by vascular smooth muscle cells
Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1869 - H1874.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Z. S. Azzam, F. J. Saldias, A. Comellas, K. M. Ridge, D. H. Rutschman, and J. I. Sznajder
Catecholamines increase lung edema clearance in rats with increased left atrial pressure
J Appl Physiol, March 1, 2001; 90(3): 1088 - 1094.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Gonin, G. Deschênes, F. Roger, M. Bens, P.-Y. Martin, J.-L. Carpentier, A. Vandewalle, A. Doucet, and E. Féraille
Cyclic AMP Increases Cell Surface Expression of Functional Na,K-ATPase Units in Mammalian Cortical Collecting Duct Principal Cells
Mol. Biol. Cell, February 1, 2001; 12(2): 255 - 264.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
T. Sakuma, C. Tuchihara, M. Ishigaki, K. Osanai, Y. Nambu, H. Toga, K. Takahashi, N. Ohya, T. Kurihara, and M. A. Matthay
Denopamine, a {beta}1-adrenergic agonist, increases alveolar fluid clearance in ex vivo rat and guinea pig lungs
J Appl Physiol, January 1, 2001; 90(1): 10 - 16.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. Feraille and A. Doucet
Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control
Physiol Rev, January 1, 2001; 81(1): 345 - 418.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. A. Frank, Y. Wang, O. Osorio, and M. A. Matthay
beta -Adrenergic agonist therapy accelerates the resolution of hydrostatic pulmonary edema in sheep and rats
J Appl Physiol, October 1, 2000; 89(4): 1255 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. G. Therien and R. Blostein
Mechanisms of sodium pump regulation
Am J Physiol Cell Physiol, September 1, 2000; 279(3): C541 - C566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
F. J. SALDÍAS, E. LECUONA, A. P. COMELLAS, K. M. RIDGE, D. H. RUTSCHMAN, and J. I. SZNAJDER
beta -Adrenergic Stimulation Restores Rat Lung Ability to Clear Edema in Ventilator-associated Lung Injury
Am. J. Respir. Crit. Care Med., July 1, 2000; 162(1): 282 - 287.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Guidot, K. Modelska, M. Lois, L. Jain, I. M. Moss, J.-F. Pittet, and L. A. S. Brown
Ethanol ingestion via glutathione depletion impairs alveolar epithelial barrier function in rats
Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L127 - L135.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. G. OLIVERA, D. E. CICCOLELLA, N. BARQUIN, K. M. RIDGE, D. H. RUTSCHMAN, D. B. YEATES, and J. I. SZNAJDER
Aldosterone Regulates Na,K-ATPase and Increases Lung Edema Clearance in Rats
Am. J. Respir. Crit. Care Med., February 1, 2000; 161(2): 567 - 573.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. L. BARNARD, K. M. RIDGE, F. SALDIAS, E. FRIEDMAN, M. GARE, C. GUERRERO, E. LECUONA, A. M. BERTORELLO, A. I. KATZ, and J. I. SZNAJDER
Stimulation of the Dopamine 1 Receptor Increases Lung Edema Clearance
Am. J. Respir. Crit. Care Med., September 1, 1999; 160(3): 982 - 986.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
C. M. Waters, K. M. Ridge, G. Sunio, K. Venetsanou, and J. I. Sznajder
Mechanical stretching of alveolar epithelial cells increases Na+-K+-ATPase activity
J Appl Physiol, August 1, 1999; 87(2): 715 - 721.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. J. Saldias, A. Comellas, K. M. Ridge, E. Lecuona, and J. I. Sznajder
Isoproterenol improves ability of lung to clear edema in rats exposed to hyperoxia
J Appl Physiol, July 1, 1999; 87(1): 30 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
V. Dumasius, J. I. Sznajder, Z. S. Azzam, J. Boja, G. M. Mutlu, M. B. Maron, and P. Factor
{beta}2-Adrenergic Receptor Overexpression Increases Alveolar Fluid Clearance and Responsiveness to Endogenous Catecholamines in Rats
Circ. Res., November 9, 2001; 89(10): 907 - 914.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online