| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Trauma Surgery, University of Freiburg, D-79106 Freiburg, Germany; 2 Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109; and 3 Department of Surgery, University of Louisville School of Medicine, Louisville, Kentucky 40202
We evaluated the roles of the C-X-C chemokines cytokine-induced neutrophil chemoattractant (CINC) and macrophage inflammatory protein-2 (MIP-2) as well as the complement activation product C5a in development of lung injury after hindlimb ischemia-reperfusion in rats. During reperfusion, CD11b and CD18, but not CD11a, were upregulated on neutrophils [bronchoalveolar lavage (BAL) and blood] and lung macrophages. BAL levels of CINC and MIP-2 were increased during the ischemic and reperfusion periods. Treatment with either anti-CINC or anti-MIP-2 IgG significantly reduced lung vascular permeability and decreased lung myeloperoxidase content by 93 and 68%, respectively (P < 0.05). During the same period, there were significant increases in serum C5a-related neutrophil chemotactic activity. Treatment with anti-C5a decreased lung vascular permeability, lung myeloperoxidase, and BAL CINC by 51, 58, and 23%, respectively (P < 0.05). The data suggest that the C-X-C chemokines CINC and MIP-2 as well as the complement activation product C5a are required for lung neutrophil recruitment and full induction of lung injury after hindlimb ischemia-reperfusion in rats.
neutrophils; macrophage inflammatory protein-2; cytokine-induced
neutrophil chemoattractant; 
2-integrins
This article has been cited by other articles:
|
|
 |
|
  K. Ley Weird and weirder: how circulating chemokines coax neutrophils to the lung Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L463 - L464. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhao, M. C. Montalto, K. J. Pfeiffer, L. Hao, and G. L. Stahl Murine model of gastrointestinal ischemia associated with complement-dependent injury J Appl Physiol, July 1, 2002; 93(1): 338 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Lapointe, J. Frenette, and C. H. Cote Lengthening contraction-induced inflammation is linked to secondary damage but devoid of neutrophil invasion J Appl Physiol, May 1, 2002; 92(5): 1995 - 2004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Calkins, D. D. Bensard, J. K. Heimbach, X. Meng, B. D. Shames, E. J. Pulido, and R. C. McIntyre Jr. L-Arginine attenuates lipopolysaccharide-induced lung chemokine production Am J Physiol Lung Cell Mol Physiol, March 1, 2001; 280(3): L400 - L408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Brock, R. G. Nie, K. A. Harris, and R. F. Potter Kupffer cell-initiated remote hepatic injury following bilateral hindlimb ischemia is complement dependent Am J Physiol Gastrointest Liver Physiol, February 1, 2001; 280(2): G279 - G284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yoshidome, A. Kato, M. J. Edwards, and A. B. Lentsch Interleukin-10 inhibits pulmonary NF-kappa B activation and lung injury induced by hepatic ischemia-reperfusion Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L919 - L923. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
