AJP - Lung Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 276: L376-L382, 1999;
1040-0605/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mair, N.
Right arrow Articles by Dietl, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mair, N.
Right arrow Articles by Dietl, P.
Vol. 276, Issue 2, L376-L382, February 1999

Exocytosis in alveolar type II cells revealed by cell capacitance and fluorescence measurements

Norbert Mair, Thomas Haller, and Paul Dietl

Department of Physiology, University of Innsbruck, A-6020 Innsbruck, Austria

Measurement of lamellar body (LB) exocytosis at high spatial and temporal resolution was recently enabled by fluorescence of the dye FM 1-43 (FFM1-43). Here, the capabilities of this method were further examined and extended by simultaneous measurement of the cell membrane capacitance (Cm) and laser-scanning confocal microscopy. Step increases in Cm were evoked by extracellular ATP (20 µM) or an elevated pipette Ca2+ concentration (>= 3 µM). The delay between the first Cm step and the increase in FFM1-43 was <1 s, indicating ready access of FM 1-43 to exocytosed LB contents. A specific Cm of 0.88 µF/cm2 for the membrane of an exocytosed LB was calculated. Compound exocytosis was occasionally observed. Decreases in Cm, indicative of transient fusion or endocytosis, did not occur within 20 min of stimulation. Exocytosis was stimulated by 160 µM guanosine 5'-O-(3-thiotriphosphate) in the pipette, but compound exocytosis was unaffected. The comparison of methods revealed that FM 1-43 is ideally suited to measure the onset of exocytosis and amount of secretion. Patch clamp is superior in resolving fusion events with the plasma membrane.

surfactant; patch clamp; endocytosis; compound exocytosis


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Chen, J. D. Warner, D. I. Yule, and D. R. Giovannucci
Spatiotemporal analysis of exocytosis in mouse parotid acinar cells
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1209 - C1219.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Mair, M. Frick, C. Bertocchi, T. Haller, A. Amberger, H. Weiss, R. Margreiter, W. Streif, and P. Dietl
Inhibition by cytoplasmic nucleotides of a new cation channel in freshly isolated human and rat type II pneumocytes
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1284 - L1292.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Frick, C. Bertocchi, P. Jennings, T. Haller, N. Mair, W. Singer, W. Pfaller, M. Ritsch-Marte, and P. Dietl
Ca2+ entry is essential for cell strain-induced lamellar body fusion in isolated rat type II pneumocytes
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L210 - L220.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
E. Sotz, H. Niederstatter, and B. Pelster
Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla
J. Exp. Biol., April 15, 2002; 205(8): 1069 - 1075.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Frick, S. Eschertzhuber, T. Haller, N. Mair, and P. Dietl
Secretion in Alveolar Type II Cells at the Interface of Constitutive and Regulated Exocytosis
Am. J. Respir. Cell Mol. Biol., September 1, 2001; 25(3): 306 - 315.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Chander, N. Sen, and A. R. Spitzer
Synexin and GTP increase surfactant secretion in permeabilized alveolar type II cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L991 - L998.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C. Prem and B. Pelster
Swimbladder gas gland cells cultured on permeable supports regain their characteristic polarity
J. Exp. Biol., January 12, 2001; 204(23): 4023 - 4029.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Prem, W. Salvenmoser, J. Wurtz, and B. Pelster
Swim bladder gas gland cells produce surfactant: in vivo and in culture
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2000; 279(6): R2336 - R2343.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Ashino, X. Ying, L. G. Dobbs, and J. Bhattacharya
[Ca2+]i oscillations regulate type II cell exocytosis in the pulmonary alveolus
Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L5 - L13.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Haller, K. Auktor, M. Frick, N. Mair, and P. Dietl
Threshold calcium levels for lamellar body exocytosis in type II pneumocytes
Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L893 - L900.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online