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Am J Physiol Lung Cell Mol Physiol 276: L631-L641, 1999;
1040-0605/99 $5.00
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Vol. 276, Issue 4, L631-L641, April 1999

Filaments of surfactant protein A specifically interact with corrugated surfaces of phospholipid membranes

Nades Palaniyar1, Ross A. Ridsdale1, Stephen A. Hearn2, Yew Meng Heng3, F. Peter Ottensmeyer3, Fred Possmayer4, and George Harauz1

1 Department of Molecular Biology and Genetics, University of Guelph, Guelph N1G 2W1; 2 Department of Pathology, St. Joseph's Health Center, London N6A 4L6; 3 Division of Molecular and Structural Biology, Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto, Toronto M5G 2M9; and 4 Medical Research Council Group in Fetal and Neonatal Health and Development, Departments of Obstetrics and Gynaecology and Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5A5

Pulmonary surfactant, a mixture of lipids and surfactant proteins (SPs), plays an important role in respiration and gas exchange. SP-A, the major SP, exists as an octadecamer that can self-associate to form elongated protein filaments in vitro. We have studied here the association of purified bovine SP-A with lipid vesicle bilayers in vitro with negative staining with uranyl acetate and transmission electron microscopy. Native bovine surfactant was also examined by transmission electron microscopy of thinly sectioned embedded material. Lipid vesicles made from dipalmitoylphosphatidylcholine and egg phosphatidylcholine (1:1 wt/wt) generally showed a smooth surface morphology, but some large vesicles showed a corrugated one. On the smooth-surfaced vesicles, SP-As primarily interacted in the form of separate octadecamers or as multidirectional protein networks. On the surfaces of the striated vesicles, SP-As primarily formed regularly spaced unidirectional filaments. The mean spacing between adjacent striations and between adjacent filaments was 49 nm. The striated surfaces were not essential for the formation of filaments but appeared to stabilize them. In native surfactant preparations, SP-A was detected in the dense layers. This latter arrangement of the lipid bilayer-associated SP-As supported the potential relevance of the in vitro structures to the in vivo situation.

pulmonary surfactant; tubular myelin; lipid-protein interaction; membrane structure; ripple phase


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 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Palaniyar, R. A. Ridsdale, S. A. Hearn, F. Possmayer, and G. Harauz
Formation of membrane lattice structures and their specific interactions with surfactant protein A
Am J Physiol Lung Cell Mol Physiol, April 1, 1999; 276(4): L642 - L649.
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