Vol. 277, Issue 2, L292-L300, August 1999
Asbestos exposure upregulates the adhesion of pleural
leukocytes to pleural mesothelial cells via VCAM-1
Nonghoon
Choe1,
Jun
Zhang1,
Akitaka
Iwagaki2,
Shogo
Tanaka1,
David R.
Hemenway3, and
Elliott
Kagan1
1 Department of Pathology and
2 Department of Medicine, F. Edward Hébert School of Medicine, Uniformed Services
University of the Health Sciences, Bethesda, Maryland 20814-4799;
and 3 Department of Civil and
Environmental Engineering, University of Vermont, Burlington, Vermont
05405
This study was designed to assess the effects of
in vitro and in vivo asbestos exposure on the adhesion of rat pleural
leukocytes (RPLs) labeled with the fluorochrome calcein AM to rat
pleural mesothelial cells (RPMCs). Exposure of RPMCs for 24 h
to either crocidolite or chrysotile fibers (1.25-10
µg/cm2) increased
the adhesion of RPLs to RPMCs in a dose-dependent fashion, an effect
that was potentiated by interleukin-1
. These findings were not
observed with nonfibrogenic carbonyl iron particles. Crocidolite and
chrysotile plus interleukin-1 also upregulated vascular cell
adhesion molecule-1 mRNA and protein expression in RPMCs, and the
binding of RPL to asbestos-treated RPMCs was abrogated by anti-vascular
cell adhesion molecule-1 antibody. PRLs exposed by intermittent
inhalation to crocidolite for 2 wk manifested significantly greater
binding to RPMCs than did RPLs from sham-exposed animals. The ability
of asbestos fibers to upregulate RPL adhesion to RPMCs may play a role
in the induction and/or potentiation of asbestos-induced pleural injury.
crocidolite; chrysotile; intercellular adhesion molecule-1; vascular cell adhesion molecule-1
