AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 277: L362-L371, 1999;
1040-0605/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamaya, M.
Right arrow Articles by Sasaki, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamaya, M.
Right arrow Articles by Sasaki, H.
Vol. 277, Issue 2, L362-L371, August 1999

Infection of human respiratory submucosal glands with rhinovirus: effects on cytokine and ICAM-1 production

Mutsuo Yamaya1, Kiyohisa Sekizawa1, Tomoko Suzuki1, Norihiro Yamada1, Masayuki Furukawa2, Satoshi Ishizuka1, Katsutoshi Nakayama1, Masanori Terajima1, Yoshio Numazaki3, and Hidetada Sasaki1

1 Department of Geriatric Medicine and 2 Department of Otorhinolaryngology, Tohoku University School of Medicine, Sendai 980-8574; and 3 Clinical Research Division, Virus Center, Sendai National Hospital, Sendai 980-0045, Japan

To further understand the early biochemical events that occur in infected surface epithelium, we developed for the first time a model in which a respiratory submucosal gland cell population can be infected with rhinovirus (RV). Viral infection was confirmed by demonstrating with PCR that viral titers in supernatants and lysates from infected cells increased with time. Infection by RV14 upregulated the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA, the major RV receptor, on submucosal gland cells, and it increased production of interleukin (IL)-1alpha , IL-1beta , IL-6, IL-8, tumor necrosis factor-alpha , and granulocyte-macrophage colony-stimulating factor in supernatants. Antibodies to ICAM-1 inhibited RV infection of submucosal gland cells and decreased the production of cytokines after RV infection. Both IL-1alpha and IL-1beta upregulated ICAM-1 mRNA expression and increased susceptibility to RV infection, whereas other cytokines failed to alter ICAM-1 mRNA expression. Furthermore, neutralizing antibodies to IL-1alpha and IL-1beta significantly decreased the viral titers in supernatants and ICAM-1 mRNA expression after RV infection, but a neutralizing antibody to tumor necrosis factor-alpha was without effect. These findings suggest that respiratory submucosal gland cells play an important role in the initial stages of inflammation and provide useful insights into the pathogenesis of RV infection.

intercellular adhesion molecule-1; asthma; common cold; airway inflammation; interleukin-1; polymerase chain reaction


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. C. Newcomb, U. S. Sajjan, D. R. Nagarkar, A. M. Goldsmith, J. K. Bentley, and M. B. Hershenson
Cooperative effects of rhinovirus and TNF-{alpha} on airway epithelial cell chemokine expression
Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L1021 - L1028.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. K. Bentley, D. C. Newcomb, A. M. Goldsmith, Y. Jia, U. S. Sajjan, and M. B. Hershenson
Rhinovirus Activates Interleukin-8 Expression via a Src/p110{beta} Phosphatidylinositol 3-Kinase/Akt Pathway in Human Airway Epithelial Cells
J. Virol., February 1, 2007; 81(3): 1186 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Lopez-Souza, G. Dolganov, R. Dubin, L. A. Sachs, L. Sassina, H. Sporer, S. Yagi, D. Schnurr, H. A. Boushey, and J. H. Widdicombe
Resistance of differentiated human airway epithelium to infection by rhinovirus
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L373 - L381.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online