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Am J Physiol Lung Cell Mol Physiol 277: L719-L726, 1999;
1040-0605/99 $5.00
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Vol. 277, Issue 4, L719-L726, October 1999

Modeling the interactions of particulates with epithelial lining fluid antioxidants

Henryk Zielinski1, Ian S. Mudway2, Kelly A. Bérubé3, Samantha Murphy3, Roy Richards3, and Frank J. Kelly2

1 Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland; 2 The Rayne Institute, St. Thomas' Hospital, London SE1 7EH; and 3 Cardiff School of Biosciences, Cardiff CF1 1ST, United Kingdom

Oxidative stress may be a fundamental mode of injury associated with inspired particles. To examine this, we determined the ability of three carbon black particles (CBPs; M120, M880, and R250) and two forms of silicon dioxide, amorphous (Cabosil) and crystalline (DQ12) quartz, to deplete epithelium lining fluid antioxidant defenses. Single and composite antioxidant solutions of uric acid, ascorbic acid (AA), and reduced glutathione (GSH) were examined in the presence of particle concentrations of 150 µg/ml. Uric acid was not depleted by any particle considered. AA was depleted in a near-linear fashion with time by the three different CBPs; however, AA depletion rates varied markedly with CBP type and decreased in the presence of metal chelators. An initially high GSH depletion rate was noted with all CBPs, and this was always accompanied by the appearance of oxidized glutathione. Exposure to Cabosil or DQ12 did not result in the loss of GSH. Together, these data demonstrate that particle type, size, and surface area are all important factors when considering particle-antioxidant interactions in the airways.

oxidative stress; particles; air pollution; ascorbic acid; reduced glutathione


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