AJP - Lung Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 277: L787-L793, 1999;
1040-0605/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, J.
Right arrow Articles by Block, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, J.
Right arrow Articles by Block, E. R.
Vol. 277, Issue 4, L787-L793, October 1999

Nitric oxide-induced reduction of lung cell and whole lung thioredoxin expression is regulated by NF-kappa B

Jianliang Zhang1, Leonard W. Velsor2, Jawaharlal M. Patel1,3, Edward M. Postlethwait2, and Edward R. Block1,3

1 Department of Medicine, University of Florida, and 3 Research Service, Malcom Randall Department of Veterans Affairs Medical Center, Gainesville, Florida 32608; and 2 Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas 77555

We examined whether nitric oxide (NO)-induced inhibition of thioredoxin (Thx) expression is regulated by a mechanism mediated by a transcription factor, i.e., nuclear factor-kappa B (NF-kappa B), in cultured porcine pulmonary artery endothelial cells (PAEC) and in mouse lungs. Western blot analysis revealed that Ikappa B-alpha content was reduced by 20 and 60% in PAEC exposed to 8.5 ppm NO for 2 and 24 h, respectively. NO exposure also caused significant reductions of cytosol fraction p65 and p52 content in PAEC. The nuclear fraction p65 and p52 contents were significantly reduced only in PAEC exposed to NO for 24 h. Exposure to NO resulted in a 50% reduction of p52 mRNA but not of the Ikappa B-alpha subunit. DNA binding activity of the oligonucleotide encoding the NF-kappa B sequence in the Thx gene was significantly reduced in PAEC exposed to NO for 24 h. Exposure of mice to 10 ppm NO for 24 h resulted in a significant reduction of lung Thx and Ikappa B-alpha mRNA and protein expression and in the oligonucleotide encoding Thx and NF-kappa B/DNA binding. These results 1) demonstrate that the effects of NO exposure on Thx expression in PAEC are comparable to those observed in intact lung and 2) suggest that reduced expression of the NF-kappa B subunit, leading to reduced NF-kappa B/DNA binding, is associated with the loss of Thx expression in PAEC and in intact mouse lungs.

nuclear factor-kappa B; transcription factor; gene regulation; mouse lung; lung endothelium


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Zhang, B. Jin, L. Li, E. R. Block, and J. M. Patel
Nitric oxide-induced persistent inhibition and nitrosylation of active site cysteine residues of mitochondrial cytochrome-c oxidase in lung endothelial cells
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C840 - C849.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Zhang, S.-L. Xia, E. R. Block, and J. M. Patel
NO upregulation of a cyclic nucleotide-gated channel contributes to calcium elevation in endothelial cells
Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1080 - C1089.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Connelly, M. Palacios-Callender, C. Ameixa, S. Moncada, and A. J. Hobbs
Biphasic Regulation of NF-{{kappa}}B Activity Underlies the Pro- and Anti-Inflammatory Actions of Nitric Oxide
J. Immunol., March 15, 2001; 166(6): 3873 - 3881.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. P. Cherla and R. K. Ganju
Stromal Cell-Derived Factor 1{{alpha}}-Induced Chemotaxis in T Cells Is Mediated by Nitric Oxide Signaling Pathways
J. Immunol., March 1, 2001; 166(5): 3067 - 3074.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online