AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 277: L816-L822, 1999;
1040-0605/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lorenz, R. R.
Right arrow Articles by Jones, K. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lorenz, R. R.
Right arrow Articles by Jones, K. A.
Vol. 277, Issue 4, L816-L822, October 1999

Hydrogen peroxide decreases Ca2+ sensitivity in airway smooth muscle by inhibiting rMLC phosphorylation

Robert R. Lorenz, David O. Warner, and Keith A. Jones

Departments of Anesthesiology and Physiology and Biophysics, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905

The purpose of this study was to determine the mechanism by which hydrogen peroxide (H2O2), an important inflammatory mediator, relaxes canine tracheal smooth muscle (CTSM). H2O2 caused concentration-dependent relaxations of CTSM strips contracted with ACh or isotonic KCl [EC50 of 0.24 ± 0.04 (SE) and 0.23 ± 0.04 mM, respectively]. Indomethacin (10 µM) decreased the sensitivity of both KCl- and ACh-contracted strips to H2O2. H2O2 increased intracellular cAMP levels, an increase that was abolished by indomethacin. H2O2 did not affect intracellular cGMP levels. In strips treated with indomethacin and contracted with ACh or isotonic KCl, H2O2-evoked relaxations were accompanied by increases in intracellular Ca2+ concentration and decreases in regulatory myosin light chain phosphorylation. We conclude that H2O2 decreases Ca2+ sensitivity in CTSM by decreasing regulatory myosin light chain phosphorylation through inhibition of myosin light chain kinase and/or activation of smooth muscle protein phosphatases.

lung; trachea; canine tracheal smooth muscle; acetylcholine; guanosine 3',5'-cyclic monophosphate; adenosine 3',5'-cyclic monophosphate; regulatory myosin light chain


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Chitano, L. Wang, S. N. Mason, R. L. Auten, E. N. Potts, W. M. Foster, A. Sturrock, T. P. Kennedy, J. R. Hoidal, and T. M. Murphy
Airway smooth muscle relaxation is impaired in mice lacking the p47phox subunit of NAD(P)H oxidase
Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L139 - L148.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. Bai and M. J. Sanderson
Modulation of the Ca2+ sensitivity of airway smooth muscle cells in murine lung slices
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L208 - L221.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. J. Perkins, R. R. Lorenz, M. Bogoger, D. O. Warner, C. R. Cremo, and K. A. Jones
A novel mechanism by which hydrogen peroxide decreases calcium sensitivity in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L324 - L332.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online