AJP - Lung Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 277: L943-L951, 1999;
1040-0605/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laporte, J. D.
Right arrow Articles by Shore, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laporte, J. D.
Right arrow Articles by Shore, S. A.
Vol. 277, Issue 5, L943-L951, November 1999

Role of ERK MAP kinases in responses of cultured human airway smooth muscle cells to IL-1beta

Johanne D. Laporte1, Paul E. Moore1, Joseph H. Abraham1, Geoffrey N. Maksym1, Ben Fabry1, Reynold A. Panettieri Jr.2, and Stephanie A. Shore1

1 Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and 2 Pulmonary and Critical Care Division, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104

We have previously reported that interleukin (IL)-1beta causes beta -adrenergic hyporesponsiveness in cultured human airway smooth muscle cells by increasing cyclooxygenase-2 (COX-2) expression and prostanoid formation. The purpose of this study was to determine whether extracellular signal-regulated kinases (ERKs) are involved in these events. Levels of phosphorylated ERK (p42 and p44) increased 8.3- and 13-fold, respectively, 15 min after treatment with IL-1beta (20 ng/ml) alone. Pretreating cells with the mitogen-activated protein kinase kinase inhibitor PD-98059 or U-126 (2 h before IL-1beta treatment) decreased ERK phosphorylation. IL-1beta (20 ng/ml for 22 h) alone caused a marked induction of COX-2 and increased basal PGE2 release 28-fold (P < 0.001). PD-98059 (100 µM) and U-126 (10 µM) each decreased COX-2 expression when administered before IL-1beta treatment. In control cells, PD-98059 and U-126 had no effect on basal or arachidonic acid (AA; 10 µM)-stimulated PGE2 release, but both inhibitors caused a significant decrease in bradykinin (BK; 1 µM)-stimulated PGE2 release, consistent with a role for ERK in the activation of phospholipase A2 by BK. In IL-1beta -treated cells, prior administration of PD-98059 caused 81, 92 and 40% decreases in basal and BK- and AA-stimulated PGE2 release, respectively (P < 0.01), whereas administration of PD-98059 20 h after IL-1beta resulted in only 38 and 43% decreases in basal and BK-stimulated PGE2 release, respectively (P < 0.02) and had no effect on AA-stimulated PGE2 release. IL-1beta attenuated isoproterenol-induced decreases in human airway smooth muscle stiffness as measured by magnetic twisting cytometry, and PD-98059 or U-126 abolished this effect in a concentration-dependent manner. These results are consistent with the hypothesis that ERKs are involved early in the signal transduction pathway through which IL-1beta induces PGE2 synthesis and beta -adrenergic hyporesponsiveness and that ERKs act by inducing COX-2 and activating phospholipase A2.

extracellular signal-regulated kinase; mitogen-activated protein; interleukin-1beta ; prostaglandin E2; beta -adrenergic responses; PD-98059; U-126; magnetic twisting cytometry; cyclooxygenase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Hu, G. Nino, J. S. Grunstein, S. Fatma, and M. M. Grunstein
Prolonged heterologous {beta}2-adrenoceptor desensitization promotes proasthmatic airway smooth muscle function via PKA/ERK1/2-mediated phosphodiesterase-4 induction
Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1055 - L1067.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. M. Pascual, E. M. Carr, M. C. Seeds, M. Guo, R. A. Panettieri Jr., S. P. Peters, and R. B. Penn
Regulatory features of interleukin-1beta-mediated prostaglandin E2 synthesis in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L501 - L508.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. S. Faffe, L. Flynt, M. Mellema, T. R. Whitehead, K. Bourgeois, R. A. Panettieri Jr., E. S. Silverman, and S. A. Shore
Oncostatin M causes VEGF release from human airway smooth muscle: synergy with IL-1{beta}
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1040 - L1048.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Baraldo, D. S. Faffe, P. E. Moore, T. Whitehead, M. McKenna, E. S. Silverman, R. A. Panettieri Jr., and S. A. Shore
Interleukin-9 influences chemokine release in airway smooth muscle: role of ERK
Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L1093 - L1102.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Lahiri, P. E. Moore, S. Baraldo, T. R. Whitehead, M. D. McKenna, R. A. Panettieri Jr., and S. A. Shore
Effect of IL-1beta on CRE-dependent gene expression in human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1239 - L1246.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
W. T. Gerthoffer and C. A. Singer
Secretory Functions of Smooth Muscle: Cytokines and Growth Factors
Mol. Interv., November 1, 2002; 2(7): 447 - 456.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Koumas and R. P. Phipps
Differential COX localization and PG release in Thy-1+ and Thy-1- human female reproductive tract fibroblasts
Am J Physiol Cell Physiol, August 1, 2002; 283(2): C599 - C608.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
M. Satoh, A. D. Parent, and J. H. Zhang
Inhibitory Effect With Antisense Mitogen-Activated Protein Kinase Oligodeoxynucleotide Against Cerebral Vasospasm in Rats
Stroke, March 1, 2002; 33(3): 775 - 781.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. M. Pascual, C. K. Billington, I. P. Hall, R. A. Panettieri Jr., J. E. Fish, S. P. Peters, and R. B. Penn
Mechanisms of cytokine effects on G protein-coupled receptor-mediated signaling in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1425 - L1435.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. D. Finder, J. L. Petrus, A. Hamilton, R. T. Villavicencio, B. R. Pitt, and S. M. Sebti
Signal transduction pathways of IL-1{beta}-mediated iNOS in pulmonary vascular smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L816 - L823.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Puig-De-Morales, M. Grabulosa, J. Alcaraz, J. Mullol, G. N. Maksym, J. J. Fredberg, and D. Navajas
Measurement of cell microrheology by magnetic twisting cytometry with frequency domain demodulation
J Appl Physiol, September 1, 2001; 91(3): 1152 - 1159.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. E. Moore, T. Lahiri, J. D. Laporte, T. Church, R. A. Panettieri Jr., and S. A. Shore
Signal Transduction in Smooth Muscle: Selected Contribution: Synergism between TNF-{alpha} and IL-1{beta} in airway smooth muscle cells: implications for {beta}-adrenergic responsiveness
J Appl Physiol, September 1, 2001; 91(3): 1467 - 1474.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. P. HALLSWORTH, L. M. MOIR, D. LAI, and S. J. HIRST
Inhibitors of Mitogen-activated Protein Kinases Differentially Regulate Eosinophil-activating Cytokine Release from Human Airway Smooth Muscle
Am. J. Respir. Crit. Care Med., August 15, 2001; 164(4): 688 - 697.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Lahiri, J. D. Laporte, P. E. Moore, R. A. Panettieri Jr., and S. A. Shore
Interleukin-6 family cytokines: signaling and effects in human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1225 - L1232.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. D. Laporte, P. E. Moore, T. Lahiri, I. N. Schwartzman, R. A. Panettieri Jr., and S. A. Shore
p38 MAP kinase regulates IL-1beta responses in cultured airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L932 - L941.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. E. Caughey, L. G. Cleland, J. R. Gamble, and M. J. James
Up-regulation of Endothelial Cyclooxygenase-2 and Prostanoid Synthesis by Platelets. ROLE OF THROMBOXANE A2
J. Biol. Chem., October 5, 2001; 276(41): 37839 - 37845.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. E. Moore, T. L. Church, D. D. Chism, R. A. Panettieri Jr., and S. A. Shore
IL-13 and IL-4 cause eotaxin release in human airway smooth muscle cells: a role for ERK
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L847 - L853.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online