AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 278: L165-L176, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dupuit, F.
Right arrow Articles by Puchelle, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dupuit, F.
Right arrow Articles by Puchelle, E.
Vol. 278, Issue 1, L165-L176, January 2000

Differentiated and functional human airway epithelium regeneration in tracheal xenografts

F. Dupuit, D. Gaillard, J. Hinnrasky, E. Mongodin, S. de Bentzmann, E. Copreni, and E. Puchelle

Institut National de la Santé et de la Recherche Médicale Unité 514, Université de Reims, Institut Federatif de Recherche 53, Centre Hospitalier Universitaire Maison Blanche, 51092 Reims Cedex, France

To investigate the regeneration process of a well-differentiated and functional human airway epithelium, we adapted an in vivo xenograft model in which adult human nasal epithelial cells adhere and progressively repopulate denuded rat tracheae grafted in nude mice. The proliferating activity, the degree of differentiation, and the barrier integrity of the repopulated epithelium were studied during the regeneration process at optical and ultrastructural levels with immunocytochemistry and a permeability tracer. Three days after implantation in nude mice, tracheal xenografts were partially repopulated with a flattened nonciliated and poorly differentiated leaky epithelium. By the end of the first week after the graft, cell proliferation produced on the entire surface of the rat trachea an epithelium that was stratified into multiple layers and tightly sealed. During successive weeks, cell proliferation dramatically decreased. Moreover, the epithelium became progressively columnar, secretory, ciliated, and transiently leaky. At 4-5 wk, a fully differentiated pseudostratified functional epithelial barrier impermeable to a low-molecular-weight tracer was reconstituted. The regeneration of a well-differentiated and functional human airway epithelium in rat tracheae grafted in nude mice includes several steps that mimic the regeneration dynamics of airway epithelium after injury.

cell proliferation; cell differentiation; epithelial barrier integrity; nude mice


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. LeSimple, I. van Seuningen, M.-P. Buisine, M.-C. Copin, M. Hinz, W. Hoffmann, R. Hajj, S. L. Brody, C. Coraux, and E. Puchelle
Trefoil Factor Family 3 Peptide Promotes Human Airway Epithelial Ciliated Cell Differentiation
Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 296 - 303.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. Puchelle, J.-M. Zahm, J.-M. Tournier, and C. Coraux
Airway Epithelial Repair, Regeneration, and Remodeling after Injury in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2006; 3(8): 726 - 733.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. L. Rawlins and B. L. M. Hogan
Epithelial stem cells of the lung: privileged few or opportunities for many?
Development, July 1, 2006; 133(13): 2455 - 2465.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
C. Coraux, R. Hajj, P. Lesimple, and E. Puchelle
In vivo models of human airway epithelium repair and regeneration
Eur. Respir. Rev., December 1, 2005; 14(97): 131 - 136.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Ferkol, L. A. Cohn, T. E. Phillips, A. Smith, and P. B. Davis
Targeted Delivery of Antiprotease to the Epithelial Surface of Human Tracheal Xenografts
Am. J. Respir. Crit. Care Med., May 15, 2003; 167(10): 1374 - 1379.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. Escotte, C. Danel, D. Gaillard, S. Benoit, J. Jacquot, D. Dusser, J.-M. Triglia, C. Majer-Teboul, and E. Puchelle
Fluticasone Propionate Inhibits Lipopolysaccharide-Induced Proinflammatory Response in Human Cystic Fibrosis Airway Grafts
J. Pharmacol. Exp. Ther., September 1, 2002; 302(3): 1151 - 1157.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. C. Devor, R. J. Bridges, and J. M. Pilewski
Pharmacological modulation of ion transport across wild-type and Delta F508 CFTR-expressing human bronchial epithelia
Am J Physiol Cell Physiol, August 1, 2000; 279(2): C461 - C479.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online