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v
3-integrin in lung and other
organs
Department of Physiology and Cellular Biophysics, Department of Medicine, and St. Luke's-Roosevelt Institute of Health Sciences, College of Physicians and Surgeons, Columbia University, New York, New York 10019
The expression of
the 
v
3-integrin in nonproliferating
vascular beds remains unclear. To determine possible organ-specific differences, we compared v3-integrin
expression in the lung and other organs. Paraffin-embedded tissue
sections of lung, liver, brain, muscle and skin obtained from rats were
processed for immunohistochemistry with a monoclonal (LM609) and a
polyclonal antibody (AB1903) against the
v3-integrin. Immunogold electron
microscopy was used to localize
v3-integrin in rat lung microvasculature.
With the use of custom-designed primers, lung sections were subjected
to in situ PCR in a thermal cycler to amplify v
or 3 mRNA. To confirm specific amplification, PCR
products were further hybridized in situ with an v or
3 cDNA probe. In the lung, the
v3-integrin protein as well as
v and 3 mRNAs was extensively evident in the endothelium of extra-alveolar and alveolar microvessels, in vascular smooth muscle, and in large bronchial epithelium but not in
the epithelium of alveolar ducts or alveoli. Ultrastructural immunogold
labeling showed the presence of the integrin on the luminal and
abluminal faces of the lung microvascular endothelium but not on the
apical surface of the alveolar epithelium. Staining for the integrin
was generally negative in blood vessels of several systemic organs,
although weak staining was evident in branches of the hepatic portal
vein. The constitutive presence of the v and
3 mRNAs and the
v3-integrin in the lung microvascular bed suggests that gene transcription for the integrin is ongoing in lung
vessels. Because it binds vitronectin, the lung vascular v3-integrin may play a role in ligation
of bloodborne, vitronectin-containing macromolecular complexes formed
in inflammation.
lung endothelium; in situ polymerase chain reaction; immunohistochemistry
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