AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 278: L51-L58, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Wetering, S.
Right arrow Articles by Hiemstra, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Wetering, S.
Right arrow Articles by Hiemstra, P. S.
Vol. 278, Issue 1, L51-L58, January 2000

Regulation of SLPI and elafin release from bronchial epithelial cells by neutrophil defensins

Sandra van Wetering1, Abraham C. van der Linden1, Marianne A. J. A. van Sterkenburg1, Willem I. de Boer1, Astrid L. A. Kuijpers2, Joost Schalkwijk2, and Pieter S. Hiemstra1

1 Department of Pulmonology, Leiden University Medical Center, 2300 RC Leiden; and 2 Department of Dermatology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands

Secretory leukocyte proteinase inhibitor (SLPI) is a serine proteinase inhibitor that is produced locally in the lung by cells of the submucosal bronchial glands and by nonciliated epithelial cells. Its main function appears to be the inhibition of neutrophil elastase (NE). Recently, NE was found to enhance SLPI mRNA levels while decreasing SLPI protein release in airway epithelial cells. Furthermore, glucocorticoids were shown to increase both constitutive and NE-induced SLPI mRNA levels. In addition to NE, stimulated neutrophils also release alpha -defensins. Defensins are small, antimicrobial polypeptides that are found in high concentrations in purulent secretions of patients with chronic airway inflammation. Like NE, defensins induce interleukin-8 production in airway epithelial cells. This induction is sensitive to inhibition by the glucocorticoid dexamethasone and is prevented in the presence of alpha 1-proteinase inhibitor. The aim of the present study was to investigate the effect of defensins on the production of SLPI and the related NE inhibitor elafin/SKALP in primary bronchial epithelial cells (PBECs). Defensins significantly increase SLPI protein release by PBECs in a time- and dose-dependent fashion without affecting SLPI mRNA synthesis. In the presence of alpha 1-proteinase inhibitor, the defensin-induced SLPI protein release is further enhanced, but no effect was observed on SLPI mRNA levels. Dexamethasone did not affect SLPI protein release from control or defensin-treated PBECs. In addition, we observed a constitutive release of elafin/SKALP by PBECs, but this was not affected by defensins. The present results suggest a role for defensins in the dynamic regulation of the antiproteinase screen in the lung at sites of inflammation.

secretory leukocyte proteinase inhibitor; inflammation; proteinase inhibitors


This article has been cited by other articles:


Home page
 Vet PatholHome page
B. Grubor, D. K. Meyerholz, and M. R. Ackermann
Collectins and cationic antimicrobial peptides of the respiratory epithelia.
Vet. Pathol., September 1, 2006; 43(5): 595 - 612.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. B. Vos, M. A. van Sterkenburg, K. F. Rabe, J. Schalkwijk, P. S. Hiemstra, and N. A. Datson
Transcriptional response of bronchial epithelial cells to Pseudomonas aeruginosa: identification of early mediators of host defense
Physiol Genomics, May 11, 2005; 21(3): 324 - 336.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
R. P. Schleimer
Glucocorticoids Suppress Inflammation but Spare Innate Immune Responses in Airway Epithelium
Proceedings of the ATS, November 1, 2004; 1(3): 222 - 230.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Aarbiou, R. M. Verhoosel, S. van Wetering, W. I. de Boer, J. H. J. M. van Krieken, S. V. Litvinov, K. F. Rabe, and P. S. Hiemstra
Neutrophil Defensins Enhance Lung Epithelial Wound Closure and Mucin Gene Expression In Vitro
Am. J. Respir. Cell Mol. Biol., February 1, 2004; 30(2): 193 - 201.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. Ganz
Antimicrobial polypeptides
J. Leukoc. Biol., January 1, 2004; 75(1): 34 - 38.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. S. Tjabringa, J. Aarbiou, D. K. Ninaber, J. W. Drijfhout, O. E. Sorensen, N. Borregaard, K. F. Rabe, and P. S. Hiemstra
The Antimicrobial Peptide LL-37 Activates Innate Immunity at the Airway Epithelial Surface by Transactivation of the Epidermal Growth Factor Receptor
J. Immunol., December 15, 2003; 171(12): 6690 - 6696.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. Aarbiou, M. Ertmann, S. van Wetering, P. van Noort, D. Rook, K. F. Rabe, S. V. Litvinov, J. H. J. M. van Krieken, W. I. de Boer, and P. S. Hiemstra
Human neutrophil defensins induce lung epithelial cell proliferation in vitro
J. Leukoc. Biol., July 1, 2002; 72(1): 167 - 174.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. R. White, P. Williams, K. R. Wojcik, S. Sun, P. S. Hiemstra, K. F. Rabe, and D. R. Dorscheid
Initiation of Apoptosis by Actin Cytoskeletal Derangement in Human Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 282 - 294.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online