AJP - Lung AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 278: L365-L373, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fisher, J. H.
Right arrow Articles by Ikegami, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fisher, J. H.
Right arrow Articles by Ikegami, M.
Vol. 278, Issue 2, L365-L373, February 2000

Pulmonary-specific expression of SP-D corrects pulmonary lipid accumulation in SP-D gene-targeted mice

James H. Fisher1, Vladimir Sheftelyevich1, Ye-Shih Ho2, Suzanne Fligiel3, Francis X. McCormack4, Thomas R. Korfhagen5, Jeffrey A. Whitsett5, and Machiko Ikegami5

1 Pulmonary/Critical Care Medicine, Denver Health Medical Center and University of Colorado Health Sciences Center, Denver, Colorado 80262; 5 Division of Pulmonary Biology, Children's Hospital Medical Center, and 4 Pulmonary and Critical Care Medicine, University of Cincinnati School of Medicine, Cincinnati, Ohio 45229-3039; 2 Institute of Chemical Toxicology, Wayne State University, and 3 Department of Pathology, John D. Dingell Veterans Affairs Medical Center, Detroit, Michigan 48201

Targeted disruption of the surfactant protein (SP) D (SP-D) gene caused a marked pulmonary lipoidosis characterized by increased alveolar lung phospholipids, demonstrating a previously unexpected role for SP-D in surfactant homeostasis. In the present study, we tested whether the local production of SP-D in the lung influenced surfactant content in SP-D-deficient [SP-D(-/-)] and SP-D wild-type [SP-D(+/+)] mice. Rat SP-D (rSP-D) was expressed under control of the human SP-C promoter, producing rSP-D, SP-D(+/+) transgenic mice. SP-D content in bronchoalveolar lavage fluid was increased 30- to 50-fold in the rSP-D, SP-D(+/+) mice compared with the SP-D(+/+) parental strain. Lung morphology, phospholipid content, and surfactant protein mRNAs were unaltered by the increased concentration of SP-D. Likewise, the production of endogenous mouse SP-D mRNA was not perturbed by the SP-D transgene. rSP-D, SP-D(+/+) mice were bred to SP-D(-/-) mice to assess whether lung-selective expression of SP-D might correct lipid homeostasis abnormalities in the SP-D(-/-) mice. Selective expression of SP-D in the respiratory epithelium had no adverse effects on lung function, correcting surfactant phospholipid content and decreasing phosphatidylcholine incorporation significantly. SP-D regulates surfactant lipid homeostasis, functioning locally to inhibit surfactant phospholipid incorporation in the lung parenchyma and maintaining alveolar phospholipid content in the alveolus. Marked increases in biologically active tissue and alveolar SP-D do not alter lung morphology, macrophage abundance or structure, or surfactant accumulation.

pulmonary surfactant; alveolar epithelium; alveolar macrophage; surfactant protein D; surfactant protein A; saturated phosphatidylcholine


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
S. Geunes-Boyer, T. N. Oliver, G. Janbon, J. K. Lodge, J. Heitman, J. R. Perfect, and J. R. Wright
Surfactant Protein D Increases Phagocytosis of Hypocapsular Cryptococcus neoformans by Murine Macrophages and Enhances Fungal Survival
Infect. Immun., July 1, 2009; 77(7): 2783 - 2794.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Ikegami, S. Grant, T. Korfhagen, R. K. Scheule, and J. A. Whitsett
Surfactant protein-D regulates the postnatal maturation of pulmonary surfactant lipid pool sizes
J Appl Physiol, May 1, 2009; 106(5): 1545 - 1552.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. Jain, E. N. Atochina-Vasserman, Y. Tomer, H. Kadire, and M. F. Beers
Surfactant Protein D Protects against Acute Hyperoxic Lung Injury
Am. J. Respir. Crit. Care Med., October 15, 2008; 178(8): 805 - 813.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
L. Brauer, C. Kindler, K. Jager, S. Sel, B. Nolle, U. Pleyer, M. Ochs, and F. P. Paulsen
Detection of Surfactant Proteins A and D in Human Tear Fluid and the Human Lacrimal System
Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 3945 - 3953.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Jain, E. Atochina-Vasserman, H. Kadire, Y. Tomer, A. Inch, P. Scott, R. C. Savani, A. J. Gow, and M. F. Beers
SP-D-deficient mice are resistant to hyperoxia
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L861 - L871.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Zhang, M. Ikegami, T. R. Korfhagen, F. X. McCormack, M. Yoshida, R. M. Senior, J. M. Shipley, S. D. Shapiro, and J. A. Whitsett
Neither SP-A nor NH2-terminal domains of SP-A can substitute for SP-D in regulation of alveolar homeostasis
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L181 - L190.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. L. Sorensen, J. Madsen, K. Kejling, I. Tornoe, O. Nielsen, P. Townsend, F. Poulain, C. H. Nielsen, K. B. M. Reid, S. Hawgood, et al.
Surfactant protein D is proatherogenic in mice
Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2286 - H2294.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Z. Vuk-Pavlovic, E. K. Mo, C. R. Icenhour, J. E. Standing, J. H. Fisher, and A. H. Limper
Surfactant protein D enhances Pneumocystis infection in immune-suppressed mice
Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L442 - L449.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. Casey, J. Kaplan, E. N. Atochina-Vasserman, A. J. Gow, H. Kadire, Y. Tomer, J. H. Fisher, S. Hawgood, R. C. Savani, and M. F. Beers
Alveolar Surfactant Protein D Content Modulates Bleomycin-induced Lung Injury
Am. J. Respir. Crit. Care Med., October 1, 2005; 172(7): 869 - 877.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
F. Zhang, W. Pao, S. M. Umphress, S. B. Jakowlew, A. M. Meyer, L. D. Dwyer-Nield, L. D. Nielsen, K. Takeda, E. W. Gelfand, J. H. Fisher, et al.
Serum Levels of Surfactant Protein D Are Increased in Mice with Lung Tumors
Cancer Res., September 15, 2003; 63(18): 5889 - 5894.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S.-J. Yong, Z. Vuk-Pavlovic, J. E. Standing, E. C. Crouch, and A. H. Limper
Surfactant Protein D-Mediated Aggregation of Pneumocystis carinii Impairs Phagocytosis by Alveolar Macrophages
Infect. Immun., April 1, 2003; 71(4): 1662 - 1671.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. J. Foster, X. Yan, D. J. Bellotto, O. W. Moe, H. K. Hagler, A. S. Estrera, and C. C. W. Hsia
Expression of epidermal growth factor and surfactant proteins during postnatal and compensatory lung growth
Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L981 - L990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. H. Fisher, J. Larson, C. Cool, and S. W. Dow
Lymphocyte Activation in the Lungs of SP-D Null Mice
Am. J. Respir. Cell Mol. Biol., July 1, 2002; 27(1): 24 - 33.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. A. Quintero, T. R. Korfhagen, and J. R. Wright
Surfactant protein A regulates surfactant phospholipid clearance after LPS-induced injury in vivo
Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L76 - L85.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Shibata, Z. Zsengeller, K. Otake, N. Palaniyar, and B. C. Trapnell
Alveolar macrophage deficiency in osteopetrotic mice deficient in macrophage colony-stimulating factor is spontaneously corrected with age and associated with matrix metalloproteinase expression and emphysema
Blood, November 1, 2001; 98(9): 2845 - 2852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, W. M. Hull, M. Yoshida, S. E. Wert, and J. A. Whitsett
SP-D and GM-CSF regulate surfactant homeostasis via distinct mechanisms
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L697 - L703.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Hawgood, J. Akiyama, C. Brown, L. Allen, G. Li, and F. R. Poulain
GM-CSF mediates alveolar macrophage proliferation and type II cell hypertrophy in SP-D gene-targeted mice
Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1148 - L1156.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, J. A. Whitsett, A. Jobe, G. Ross, J. Fisher, and T. Korfhagen
Surfactant metabolism in SP-D gene-targeted mice
Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L468 - L476.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online